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Application of bacteroides fragilis in resisting aquacultural pathogenic bacteria

A technology of Bacteroides fragilis and aquatic pathogens, applied in the field of microorganisms, can solve the problems of unclear antibacterial effects of strains, and achieve the effects of no drug resistance, broad application prospects, and strong inhibitory effects

Active Publication Date: 2016-03-30
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] (2) The antibacterial effect of the strains in common aquatic probiotic products is not clear, and the antibacterial effect is the core index for evaluating probiotics

Method used

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  • Application of bacteroides fragilis in resisting aquacultural pathogenic bacteria
  • Application of bacteroides fragilis in resisting aquacultural pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Bacteroides fragilis ZY-312 culture

[0036] Reagents and instruments are described in the table below:

[0037]

[0038] training method

[0039] Step 1: Take a freeze-dried bacterial strain (ZY-312, Bd-312 or ATCC25285, the method is the same, so I will not list them one by one), add 200 μL of TSB medium, redissolve, draw 20 μL of blood plate, and use After the anaerobic tank gas control system pumps air, in the biochemical incubator at 37°C, anaerobic culture for 48h;

[0040] Step 2: Pick a monoclonal colony and insert it into 10mL of TSB medium, add 5% (v / v) peptide bovine serum, and culture in a biochemical incubator at 37°C for 12h anaerobically;

[0041] Step 3: Take a bottle of 500mL TSB medium, add 5% (v / v) peptide bovine serum respectively, insert 1% (v / v) strains, and culture in a biochemical incubator at 37°C for 48 hours anaerobically;

[0042] Step 4: Take the bacterial liquid and centrifuge it with a centrifuge, the centrifugation cond...

Embodiment 2

[0044] Example 2 Inhibition of Bacteroides fragilis to aquatic pathogens

[0045] 1. Pathogen plate preparation

[0046] Step 1: Cultivate Vibrio parahaemolyticus, Vibrio splendidus, Vibrio harveyi, Vibrio anguillarum or Pseudomonas aeruginosa with TSB liquid culture based on 28°C to OD 600 =0.8-1.0;

[0047] Step 2: Take 200 μL of the culture medium and centrifuge in a centrifuge at 6000-8000 rpm, centrifuge at room temperature for 3-5 minutes, discard the supernatant, and resuspend the precipitate with 200 μL sterile saline;

[0048] Step 3: Spread the suspensions of Vibrio parahaemolyticus, Vibrio resplendent, Vibrio harveyi, Vibrio anguillarum or Pseudomonas aeruginosa on TSA solid medium plates respectively, and fix at 28° C. for 2-3 hours respectively.

[0049] 2. Plant

[0050] Step 1: Take culture to OD 600 = 0.8-1.0 of ZY-312, Bd312 or ATCC25285 culture solution (washed and resuspended in step 2 of the preparation of the pathogenic bacteria plate), 20 μL of normal...

Embodiment 3

[0057] Example 3 Inhibition of Bacteroides fragilis ZY-312 to aquatic pathogens

[0058] 1. Training method

[0059] The culture method of Bacteroides fragilis ZY-312 is the same as that in Example 1.

[0060] 2. Sample preparation

[0061] 1) Preparation of live Bacteroides fragilis ZY-312 cells

[0062] Step 1: Take a freeze-dried strain of Bacteroides fragilis ZY-312, add 200 μL of TSB medium, redissolve, draw 20 μL of blood plate for streaking, and pump it through the gas control system of the anaerobic tank, and place it in the biochemical incubator 37℃, anaerobic culture for 48h;

[0063] Step 2: Pick a monoclonal colony and insert it into 10mL of TSB medium, add 5% (v / v) peptide bovine serum, and culture it anaerobically at 37°C for 12h in a biochemical incubator;

[0064] Step 3: Take a bottle of 500mL TSB medium, add 5% (v / v) peptide bovine serum respectively, insert 1% (v / v) strains, and culture in a biochemical incubator at 37°C for 48 hours under anaerobic cond...

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Abstract

The invention discloses an application of bacteroides fragilis in resisting aquacultural pathogenic bacteria. The application of the bacteroides fragilis in resisting the aquacultural pathogenic bacteria comprises the application of the bacteroides fragilis in preparation of an antibacterial medicine for the aquacultural pathogenic bacteria, and medicinal compositions and feed additives which contain the bacteroides fragilis. The bacteroides fragilis comprises living bacteria of bacteroides fragilis, inactivated bacteroides fragilis, bacteroides fragilis lysates and / or bacteroides fragilis culture supernatant fluid. According to the application of the bacteroides fragilis in resisting the aquacultural pathogenic bacteria disclosed by the invention, experiments prove that the bacteroides fragilis has strong inhibiting effects on the aquacultural pathogenic bacteria, such as vibrio parahaemolyticus, vibro splendidus, vibrio harveyi, vibrio anguillarum or pseudomonas aeruginosa, does not have medicine resistance, and is safe and non-toxic. Meanwhile, the medicinal compositions and the feed additives which contain the bacteroides fragilis also have the same or similar effects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the application of Bacteroides fragilis in aquaculture, in particular to the application of Bacteroides fragilis in resisting aquatic pathogens. Background technique [0002] With the continuous development of my country's aquaculture industry. The problem of diseases caused by industrialization and high-density farming has become increasingly prominent. The losses caused by aquatic diseases are as many as tens of billions of yuan every year. However, chemical therapy is still widely used in the treatment of aquatic animal diseases. The abuse of antibiotics has caused water bacteria to develop drug resistance and drug residues, which indirectly endangers human health. Therefore, more and more experts have begun to pay attention to the bioremediation of aquaculture environment by using beneficial microorganisms in the natural environment to replace the use of antibiotics. The ...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61P31/04A23K20/00
CPCA23K20/00A61K35/741C12N1/20
Inventor 智发朝白杨王晔刘洋洋王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
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