Application of bacteroides fragilis in resisting aquacultural pathogenic bacteria

A technology of Bacteroides fragilis and aquatic pathogens, applied in the field of microorganisms, can solve the problems of unclear antibacterial effects of strains, and achieve the effects of no drug resistance, broad application prospects, and strong inhibitory effects

Active Publication Date: 2016-03-30
GUANGZHOU ZHIYI PHARMA INC
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] (2) The antibacterial effect of the strains in common aquatic probiotic products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of bacteroides fragilis in resisting aquacultural pathogenic bacteria
  • Application of bacteroides fragilis in resisting aquacultural pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1 Culture of Bacteroides fragilis ZY-312

[0036] The reagents and instruments are described in the following table:

[0037]

[0038] Cultivation method

[0039] Step 1: Take a freeze-dried preservation strain (ZY-312, Bd-312 or ATCC25285, the method is the same, so we don’t list them one by one), add 200μLTSB medium, re-dissolve, draw 20μL blood plate and streak it with After pumping gas from the anaerobic tank gas control system, incubate in a biochemical incubator at 37°C for 48 hours;

[0040] Step 2: Pick a single colony and insert it into 10mL TSB medium, add 5% (v / v) peptide bovine serum, and incubate in a biochemical incubator at 37°C for 12 hours;

[0041] Step 3: Take 1 bottle of 500mL TSB medium, add 5% (v / v) peptide bovine serum, insert 1% (v / v) strains, and culture in a biochemical incubator at 37°C for 48 hours;

[0042] Step 4: Centrifuge the bacteria liquid, and centrifuge with a centrifuge under the conditions of 6000rpm and 10min. Wash twice with norma...

Example Embodiment

[0044] Example 2 Inhibition of Bacteroides fragilis on aquatic pathogens

[0045] 1. Pathogen plate preparation

[0046] Step 1: Cultivate Vibrio parahaemolyticus, Vibrio splendidus, Vibrio harveyi, Vibrio anguillarum or Pseudomonas aeruginosa with TSB liquid medium at 28℃ to OD 600 =0.8-1.0;

[0047] Step 2: Take 200μL of culture medium, centrifuge with a centrifuge under the conditions of 6000-8000rpm, centrifuge for 3-5min at room temperature, discard the supernatant, and resuspend the pellet with 200μL of sterile saline;

[0048] Step 3: Coat the suspensions of Vibrio parahaemolyticus, Vibrio splendidus, Vibrio harveyi, Vibrio anguillarum or Pseudomonas aeruginosa respectively on TSA solid medium plates, and fix them at 28°C for 2-3 hours.

[0049] 2. Point kind

[0050] Step 1: Take culture to OD 600 =0.8-1.0 ZY-312, Bd312 or ATCC25285 culture medium (washed and resuspended in step 2 of pathogen plate preparation), physiological saline (PBS) each 20μL, respectively dot on the plate ...

Example Embodiment

[0057] Example 3 Inhibition of Bacteroides fragilis ZY-312 on aquatic pathogens

[0058] 1. Training method

[0059] The cultivation method of Bacteroides fragilis ZY-312 is the same as in Example 1.

[0060] 2. Sample preparation

[0061] 1) Preparation of live bacteria of Bacteroides fragilis ZY-312

[0062] Step 1: Take a lyophilized and preserved strain of Bacteroides fragilis ZY-312, add 200μLTSB medium, reconstitute it, draw 20μL blood into the plate and streak it, after pumping through the anaerobic tank gas control system, in the biochemical incubator 37℃, anaerobic culture for 48h;

[0063] Step 2: Pick a single colony into 10mL TSB medium, add 5% (v / v) peptide bovine serum, and cultivate in a biochemical incubator at 37°C for 12 hours;

[0064] Step 3: Take 1 bottle of 500mL TSB medium, add 5% (v / v) peptide bovine serum, insert 1% (v / v) strains, and culture in a biochemical incubator at 37°C for 48 hours;

[0065] Step 4: Centrifuge the bacteria liquid, and centrifuge with a cen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses an application of bacteroides fragilis in resisting aquacultural pathogenic bacteria. The application of the bacteroides fragilis in resisting the aquacultural pathogenic bacteria comprises the application of the bacteroides fragilis in preparation of an antibacterial medicine for the aquacultural pathogenic bacteria, and medicinal compositions and feed additives which contain the bacteroides fragilis. The bacteroides fragilis comprises living bacteria of bacteroides fragilis, inactivated bacteroides fragilis, bacteroides fragilis lysates and/or bacteroides fragilis culture supernatant fluid. According to the application of the bacteroides fragilis in resisting the aquacultural pathogenic bacteria disclosed by the invention, experiments prove that the bacteroides fragilis has strong inhibiting effects on the aquacultural pathogenic bacteria, such as vibrio parahaemolyticus, vibro splendidus, vibrio harveyi, vibrio anguillarum or pseudomonas aeruginosa, does not have medicine resistance, and is safe and non-toxic. Meanwhile, the medicinal compositions and the feed additives which contain the bacteroides fragilis also have the same or similar effects.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the application of Bacteroides fragilis in aquaculture, in particular to the application of Bacteroides fragilis in resisting aquatic pathogens. Background technique [0002] With the continuous development of my country's aquaculture industry. The problem of diseases caused by industrialization and high-density farming has become increasingly prominent. The losses caused by aquatic diseases are as many as tens of billions of yuan every year. However, chemical therapy is still widely used in the treatment of aquatic animal diseases. The abuse of antibiotics has caused water bacteria to develop drug resistance and drug residues, which indirectly endangers human health. Therefore, more and more experts have begun to pay attention to the bioremediation of aquaculture environment by using beneficial microorganisms in the natural environment to replace the use of antibiotics. The ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/741A61P31/04A23K20/00
CPCA23K20/00A61K35/741C12N1/20
Inventor 智发朝白杨王晔刘洋洋王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap