Streptoseomycin and preparation method and application thereof

A technology of streptomycin and streptomyces, applied in biochemical equipment and methods, methods based on microorganisms, pharmaceutical formulations, etc., to achieve the effects of guaranteed source, overcoming drug resistance, and simple process

Inactive Publication Date: 2010-11-24
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Streptoseomycin and preparation method and application thereof
  • Streptoseomycin and preparation method and application thereof
  • Streptoseomycin and preparation method and application thereof

Examples

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Example Embodiment

[0028] Example 1: Separation and purification of Streptomyces seoulensis from marine sources

[0029] The prawns (Penaeus chinensis) were dissected under aseptic conditions. The intestines of the prawns were sterilized in 75% alcohol for 2 minutes, rinsed with sterile water for 3 times, and then grind in a sterile mortar with a small amount of sterile water. Sterile water is diluted to 10 -1 , 10 -2 , 10 -3 , 10 -4 , Respectively take 0.2 ml of each gradient dilution and spread it on Gao's No. I medium (soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 ·3H 2 O 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O0.01g, 1L distilled water), cultured in a thermostat at 28°C. After the colony grows, the spores are picked and purified and cultured to obtain actinomycetes in the intestinal tract of shrimp, which is identified as Streptomyces seoule by the Institute of Microbiology, Chinese Academy of Sciences ( Streptomyces seoulensis) is now deposited in the Culture Collection Center of the Inst...

Example Embodiment

[0030] Example 2: Liquid fermentation of Streptomyces seoulensis from marine sources

[0031] Activate the marine-derived Streptomyces seoulensis, inoculate the fresh cell mass into a 1000 mL Erlenmeyer flask, each flask contains 400 mL of Gao’s medium, inoculate 5-6 flasks on a shaker, at 150rpm, 28℃ Cultivate for 5 days under the conditions as seed liquid, then inoculate 20 mL of seed liquid in a 1000 mL conical flask containing 400 mL of Gaussian or Martin medium, and ferment for 8 days at 150 rpm and 20-30°C.

Example Embodiment

[0032] Example 3: Extraction and separation of Seoul streptoseomycin

[0033] The fermentation broth obtained in Example 2 was filtered through gauze, the filtrate was extracted with ethyl acetate, and concentrated and dried in vacuo to obtain a black crude extract F1. The crude extract F1 was suspended in water and extracted with petroleum ether and ethyl acetate in sequence. The ethyl acetate extract was concentrated to obtain extract F2. The extract F2 was subjected to silica gel column chromatography, followed by 4 times the volume of chloroform. Chloroform:methanol=100:1, chloroform:methanol=100:2, chloroform:methanol=100:4, eluted, concentrated 100:4 (chloroform:methanol) section to obtain black extract F3, and then processed extract F3 Sephadex LH-20 separation (eluent chloroform: methanol = 1:1) and the green fluorescent part of the eluate were combined to obtain light yellow extract F4. The extract F4 was separated by reversed-phase column ODS, first with 55% methanol T...

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Abstract

The invention belongs to the biological pharmacy technical field, in particular relates to streptoseomycin extracted from marine actinomycete streptomyces seoulensis liquid fermentation as well as preparation method and application thereof. Streptoseomycin is obtained by separation, purification and fermentation on streptomyces seoulensis from ocean. Streptoseomycin has strong inhibition function on anaerobe and has no toxicity on cell, thus streptoseomycin can be taken as lead compound of antibiotic production and can be applied in preparation of antibiotic medicine.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to streptoseomycin extracted from liquid fermented product of marine actinomyces (Streptomyces seoulensis) and its preparation method and application. Background technique [0002] Although the secondary metabolites of microorganisms have been widely studied, the metabolites of some special environment microorganisms have not attracted enough attention. The particularity of the marine environment has created the unique metabolic mode and metabolites of marine actinomycetes. In recent years, people continue to search for antibiotics with new mechanisms of action from actinomycetes in various marine environments. In addition to antibiotics, marine actinomycetes can also produce inhibitors of various enzymes, and the diversity of marine actinomycetes provides a rich source of secondary metabolites. [0003] With the widespread use of antibiotics, the drug resistance of pat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/18C07D491/22A61K31/407A61P31/04C12R1/465
Inventor 谭仁祥宋勇春乔红运梅亚宁刘敏吴琦
Owner NANJING UNIV
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