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Bacillus licheniformis used for controlling cunninghamia lanceolata anthracnose, and application thereof

A technology for Bacillus licheniformis and anthracnose, which is applied in the directions of application, bacteria, fungicides, etc., can solve the problems of no biological pesticide for preventing and controlling fir anthracnose, weak technology or even blank, etc., and achieves good development and application prospects, easy to industrialized production, and easy to use. saved effect

Inactive Publication Date: 2013-02-13
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] Isolation and screening of biocontrol strains with antagonistic effects on Chinese fir anthracnose from rhizosphere soil is of great significance to the control of Chinese fir anthracnose, but the relevant technology is still weak or even blank, and it has been able to effectively control Chinese fir anthracnose for more than 20 years Antagonistic bacteria have not been reported, nor have there been reports on biopesticides for the prevention and treatment of Chinese fir anthracnose

Method used

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  • Bacillus licheniformis used for controlling cunninghamia lanceolata anthracnose, and application thereof
  • Bacillus licheniformis used for controlling cunninghamia lanceolata anthracnose, and application thereof
  • Bacillus licheniformis used for controlling cunninghamia lanceolata anthracnose, and application thereof

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Embodiment 1

[0021] Example 1 Isolation of Bacillus licheniformis AM-53 Bacillus licheniformis AM-53

[0022] 1. Isolated strains

[0023] Weigh 1.0 g of the root soil sample of garden plants and put it into a triangular flask containing 99 mL of sterile water and glass beads, and shake it on a shaker for 5 minutes to make the soil sample evenly disperse in the diluent to become a soil suspension (10 -2 ). Use a 1mL sterile tip to draw 1mL of the soil suspension into a test tube filled with 9mL of sterile water, blow and suck 3 times, shake and mix (10 -3 ). Then use the same 1mL tip to draw 1mL from this tube and inject it into another test tube containing 9mL sterile water (10 -4 ), and so on to make 10 -5 , 10 -6 , 10 -7 Soil solutions of various dilutions. Pipette 0.1ml to the prepared PDA plate (peeled potato 200g / L, glucose 20g / L, agar 15g / L), smear it evenly, put it into the incubator for cultivation, record the amount of bacteria after 48h, and According to the colony sha...

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Abstract

The invention discloses a bacillus licheniformis AM-53 used for controlling cunninghamia lanceolata anthracnose, and an application thereof. The bacillus licheniformis has a collection number of CCTCC No: M2012273. The bacillus licheniformis AM-53 provided by the invention has a high inhibiting effect against colletotrichum gloeosporioides, and has an important significance in cunninghamia lanceolata anthracnose controlling. The bacillus licheniformis AM-53 has an important effect in plant disease bio-control. The strain has simple culturing conditions, and is easy to store. Industrialized production of the strain is easy. The strain had good development and application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to bacillus licheniformis AM-53 for preventing and treating Chinese fir anthracnose and its application. Background technique [0002] Chinese fir (Cunninghamia Lanceolata) is a widely distributed timber tree species in my country. It is a unique fast-growing commercial timber species in China and has a history of utilization and cultivation for more than 8,000 years. It has wide adaptability, fast growth, and good material. The main soil types in the production area are yellow soil and red soil. In the marginal areas, it is yellow brown soil, brick red soil, etc. Yellow soil has better conditions. It mainly grows in the south. Guizhou, Hunan, Guangxi, Jiangxi, etc. are the main producing areas of Chinese fir. Among them, Hunan Province is the best producing area of ​​Chinese fir. The wood is straight and the material is good. Chinese fir is full of treasures and has hig...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/10
Inventor 周国英吴毅李河路宗岩张磊闫法领
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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