Bacillus wettable powder containing brassinolide and preparation method thereof

A technology of brassinolide and wettable powder, which is applied in the field of microbial fertilizers and biological pesticides, can solve problems such as poor product quality stability, backward production technology, and impact on application effects, and achieve long shelf life of spores, stable production technology, The effect of promoting crop growth

Active Publication Date: 2016-05-11
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although there are many microbial pesticide products mainly prepared by Bacillus, generally speaking, there are still problems including the disconnection between research and development and production, difficulty in formulation, poor product quality stability, backward production technology, low product consistency, and the scale of production enterprises. In addition to some problems, problems similar to other biocontrol agents also includ

Method used

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  • Bacillus wettable powder containing brassinolide and preparation method thereof
  • Bacillus wettable powder containing brassinolide and preparation method thereof
  • Bacillus wettable powder containing brassinolide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A bacillus wettable powder containing brassinolide, the best components by weight are: 0.005 parts of brassinolide, 150 parts of Bacillus amyloliquefaciens bacteria liquid, emulsifier: nonylphenol poly 0.05 parts of oxyethylene ether, solvent: 0.75 parts of ethanol, spore protection agent: 5 parts of skimmed milk powder, 2 parts of methyl cellulose, dispersant: 3 parts of D425, wetting agent: 5 parts of sodium dodecylbenzenesulfonate, carrier: 70 parts of diatomaceous earth;

[0027] The preparation of the bacillus wettable powder containing brassinolide comprises the following steps:

[0028] 1. Preparation of Bacillus Bacteria Liquid

[0029] Take out the preservation tube of Bacillus amyloliquefaciens, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated into t...

Embodiment 2

[0034] A Bacillus wettable powder containing brassinolide The components of the wettable powder are in parts by weight: 0.002 parts of brassinolide, 120 parts of Bacillus subtilis bacterial liquid, emulsifier: nonylphenol 0.02 parts of polyoxyethylene ether, solvent: 0.3 parts of ethanol, spore protection agent: 3 parts of skimmed milk powder, 2 parts of methyl cellulose, dispersant: 3 parts of D425, wetting agent: 5 parts of sodium dodecylbenzenesulfonate, carrier : 75 parts of diatomite;

[0035] The preparation of the bacillus wettable powder containing brassinolide comprises the following steps:

[0036] 1. Preparation of Bacillus Bacteria Liquid

[0037] Take out the preservation tube of Bacillus subtilis, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated into th...

example 3

[0041] Example 3 Utilizes the test effect of the bacillus wettable powder containing brassinolide produced by example 1 in the paddy field

[0042] 1. The test was selected to be carried out in rice high-yielding areas;

[0043] 2. The test site is selected in the responsible field of a farmer in Longzhu Village, Tantou Town, Longhui County, Hunan Province;

[0044] 3. Test method: Drug usage and dosage: Test group: Dilute Bacillus amyloliquefaciens wettable powder containing brassinolide 1000 times in rice seedlings with two leaves and one heart to four leaves and one heart stage, 5-7 days after transplanting During the greening stage and the heading stage of rice opening, choose sunny days to apply pesticides. Bacillus group: Dilute the same amount of Bacillus amyloliquefaciens 1000 times and use it at the same time; Brassinolide group: Dilute the same amount of Brassinolide 1000 times for use; Blank control group: Spray the same amount of water . Each group uses an area ...

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Abstract

The invention discloses bacillus wettable powder containing brassinolide and a preparation method thereof. The bacillus wettable powder containing brassinolide is mainly prepared from brassinolide, a bacillus solution, nonyl phenol polyoxyethylene ether, ethanol, skim milk powder, methylcellulose, D425, sodium dodecyl benzene sulfonate and kieselguhr. The brassinolide is capable of improving the activity of plant based enzymes, improving the seed vigor, promoting early development, enhancing the photosynthetic efficiency, increasing the crop yield, improving the crop quality, improving the cold resistance, drought resistance, waterlogging resistance and saline-alkaline tolerance of crops, improving the stress tolerance, and alleviating pesticide phytotoxicity and chemical pollution phytotoxicity, and is capable of resisting hazards of multiple types of virus, bacteria and fungus and enhancing the crop disease resistance. The bacillus is capable of directly restraining or killing multiple types of plant diseases. The wettable powder is safe, environmentally friendly and efficient, is stable in production process, and can be widely applied to crops such as rice, flue-cured tobacco, corn, wheat, stawberries, watermelons, hot pepper, tomatoes and cucumbers.

Description

technical field [0001] The invention relates to the field of microbial fertilizers and biological pesticides, in particular to a bacillus wettable powder containing brassinolide and a preparation method thereof. Background technique [0002] American agronomist J.W.Mitchell and others studied and extracted a substance that can significantly promote the growth of bean seedlings from rapeseed pollen in 1970. It was named brassinosteroids, also known as brassin (Brassin), which is based on sterols. Plant endogenous steroid biologically active substances, British N.B.Mandava et al. refined brassinosteroids in 1978 to obtain crystals with high activity. In 1979, Grove et al determined that its chemical structure belonged to sterol lactone, so it was named brassinolide (Brassionlide). Later, dozens of bioactive brassinosteroids were extracted from other plants, collectively referred to as brassinosteroids (BRs for short), among which, the physiological activity of brassinosteroid...

Claims

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Application Information

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IPC IPC(8): A01N25/14A01N63/00A01N43/22A01P1/00A01P3/00A01P21/00
CPCA01N25/14A01N43/22A01N63/00A01N2300/00
Inventor 胡艳晖其他发明人请求不公开姓名
Owner FOSHAN YANHUI BIOTECH CO LTD
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