Bacillus coagulans solid-state fermentation production method

Abstract

The invention relates to the field of microbial fermentation, and in particular relates to a bacillus coagulans solid-state fermentation production method. The method comprises the following steps: (1) first-stage seed preparation; (2) second-stage solid-state seed inoculation; (3) third-stage solid-state seed inoculation; and (4) solid-state fermentation inoculation, wherein a formula of a solid-state fermentation material comprises the following components in percentage by mass: 70-99% of wheat bran, 1-30% of soybean meal, 0.1-10% of glucose, 0.1-10% of urea, 0.1-10% of calcium carbonate and 0.1-5% of manganese sulfate, and accessory materials are added corresponding to the mass of the wheat bran and the soybean meal. According to the method disclosed by the invention, the solid-state fermentation inoculation quantity can be reduced by 50-95% compared with that of the prior art, and a pH value is kept between 7 and 8 in a fermentation process; moreover, a high bacterium number can be achieved, dominant populations can be formed for fermentation, bacterial contamination cannot be easily caused, relatively high spore numbers and spore rates can be achieved, the spore number is 1*10<9>CFU / g to 1*10<11>CFU / g, and the maximum spore rate can reach 99%; and the method is easy in production and promotion.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a solid-state fermentation production method of bacillus coagulans. Background technique [0002] Bacillus coagulans has been included in the new feed and new feed additives within the protection period in the Ministry of Agriculture Announcement No. 1126 of the People's Republic of China "Feed Additives Category Catalog" (2008), and its approved use time is May 2004 In March, the scope of application is broiler chickens and growing and finishing pigs, indicating that its safety, effectiveness, economy and impact on the environment have passed the review of the National Feed Review Committee and approved by the Ministry of Agriculture. The U.S. FDA has also recognized it as a "generally recognized as safe" bacillus, and the application research of this bacillus in human and animal production has become a hot spot, and the effect is remarkable. [0003] The abuse of antibiot...

AI Technical Summary

Problems solved by technology

[0005] In recent years, with the vigorous development of microecological preparations, a group of Bacillus coagulans manufacturers and researchers have emerged. However, most of the production methods at home and abroad currently use liquid fermentation technology. However, liquid fermentation has many disadvantages, such as long fermentation period, Low spore formation rate, serious post-treatment sewage discharge, high cost and other problems, however, solid-state fermentation production method, high spore number and spore rate, simple process, low investment, easy operation, simple post-treatment, etc., have been widely accepted and research

Aiming at the industrialization of Bacillus coagulans, scholars at home and abroad have conducted in-depth research on the high-density culture of Bacillus coagulans liquid fermentation, and believe that the accumulation of lactic acid generated by Bacillus coagulans through homozygous fermentation in the fermentation liquid will cause a drop in pH value and damage the bacteria. The growth and the formation of spores have a strong inhibitory effect. For solid-state fermentation, there is also such a problem. How to more effectively increase the number and spore rate of Bacillus coagulans in solid-state fermentation