Trichoderma harzianum solid fermentation method, solid fermentation product, Trichoderma harzianum fungal agent, preparation method and application thereof

A technology of Trichoderma harzianum and solid fermentation, applied in the direction of application, fungicide, biocide, etc., can solve the problems of easy pollution of the environment by waste water and residue, excessive mycelium growth, and insufficient spore production, so as to promote the growth of grapes and prevent Significant effects on plant diseases and reduction of bacterial contamination

Active Publication Date: 2020-11-03
山东五福生生态工程有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Most of the Trichoderma harzianum fungal agents commonly used in agriculture are spore preparations (live fungus preparations). The key to making Trichoderma harzianum fungal agents for agriculture is the number of spores contained in the fungal agent, but it is easy to appear during the production process of Trichoderma harzianum. The mycelium grows too vigorously, but the spore production is insufficient
The traditional fermentation methods of Trichoderma mainly include liquid fermentation and solid shallow plate fermentation. Liquid fermentation mainly produces chlamydospores, but the yield is extremely low, and the waste water and residue produced are easy to pollute the environment; solid shallow plate fermentation is semi-open, which is easy to cause miscellaneous Bacterial contamination, and occupies a large area, inconvenient to operate

Method used

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  • Trichoderma harzianum solid fermentation method, solid fermentation product, Trichoderma harzianum fungal agent, preparation method and application thereof
  • Trichoderma harzianum solid fermentation method, solid fermentation product, Trichoderma harzianum fungal agent, preparation method and application thereof
  • Trichoderma harzianum solid fermentation method, solid fermentation product, Trichoderma harzianum fungal agent, preparation method and application thereof

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preparation example Construction

[0103] According to the fourth aspect of the present invention, a preparation method of a Trichoderma harzianum agent is provided, comprising the following steps: the Trichoderma harzianum spore powder is obtained by drying and separating the solid fermentation product of Trichoderma harzianum, and then mixed with the solid fermentation medium powder, the carrier Mixing to obtain Trichoderma harzianum agent; solid fermentation medium powder is obtained after the solid fermentation medium remaining after solid fermentation is crushed and separated.

[0104] Preferably, the ratio of Trichoderma harzianum spore powder: solid fermentation medium powder and the total amount of the carrier is 1: (10-20).

[0105] Typical but non-limiting ratios of the total amount of Trichoderma harzianum spore powder: solid fermentation medium powder and carrier are, for example, 1:10, 1:12, 1:14, 1:16, 1:18, or 1:20.

[0106] It should be noted that the particle size of the solid fermentation prod...

Embodiment 1

[0120] A method for solid fermentation of Trichoderma harzianum, comprising the following steps:

[0121] (1) seed activation: inoculate Trichoderma harzianum on potato dextrose agar medium, and cultivate at 25°C for 5 days to obtain activated Trichoderma harzianum seeds;

[0122] (2) Seed liquid preparation: inoculate the activated Trichoderma harzianum seeds in potato glucose liquid medium, cultivate at 28°C for 24 hours, the mycelium concentration reaches 180g / L, and obtain Trichoderma harzianum seed liquid;

[0123] (3) Seed liquid culture: inoculate the Trichoderma harzianum seed liquid in the liquid fermentation medium according to the inoculation amount of 1.5‰, and cultivate in the seed tank to obtain the Trichoderma harzianum seed liquid culture product;

[0124] The liquid fermentation medium includes the following components in parts by weight: 1 part of corn flour, 2.5 parts of glucose, 2 parts of soybean flour cake, KH 2 PO 4 0.05 part, NaCl 0.2 part, (NH 4 ) ...

Embodiment 2

[0133] A method for solid fermentation of Trichoderma harzianum, comprising the following steps:

[0134] (1) seed activation: inoculate Trichoderma harzianum on potato dextrose agar medium, and cultivate at 28°C for 3 days to obtain activated Trichoderma harzianum seeds;

[0135] (2) Seed liquid preparation: inoculate the activated Trichoderma harzianum seeds in potato glucose liquid medium, cultivate at 25°C for 48 hours, and the mycelial concentration reaches 220 g / L to obtain Trichoderma harzianum seed liquid;

[0136] (3) Seed liquid culture: inoculate the Trichoderma harzianum seed liquid in the liquid fermentation medium according to the inoculation amount of 2.5‰, and cultivate in the seed tank to obtain the Trichoderma harzianum seed liquid culture product;

[0137] The liquid fermentation medium includes the following components in parts by weight: 3 parts of corn flour, 1 part of glucose, 5 parts of soybean flour cake, KH 2 PO 4 0.04 part, NaCl 0.5 part, (NH 4 )...

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Abstract

The invention discloses a trichoderma harzianum solid fermentation method, a solid fermentation product, a trichoderma harzianum agent as well as a preparation method and an application of the trichoderma harzianum agent and relates to the technical field of microbial fermentation. According to the solid fermentation method, a solid fermentation tank is used for fermentation, and reproduction of trichoderma harzianum can be promoted, living contaminants can be reduced and the spore number of trichoderma harzianum can be increased by limiting humidity, turning and ventilation process parametersin the solid fermentation process. The trichoderma harzianum solid fermentation product prepared with the solid fermentation method is applied in the trichoderma harzianum agent, and the trichodermaharzianum agent can control grape downy mildew and grape gray mold efficiently and promote grape growth and increase of the yield of crops effectively and is a biological control preparation with great application prospects. Besides, the invention further provides the preparation method of the trichoderma harzianum agent. The method can fully utilize residues produced after solid fermentation, reduce emission of solid waste pollutants and save cost and is safe and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a solid fermentation method of Trichoderma harzianum, a solid fermentation product, a Trichoderma harzianum agent, and a preparation method and application thereof. Background technique [0002] Trichoderma harzianum belongs to anamorphic fungi, Hyphomycetes, Hyphomycetales, Hyphomycetaceae, Trichoderma sp. a member of . Trichoderma is widely distributed and mainly exists in moist habitats such as forests, sloping ditches, farmlands and grasslands. Plant residues such as soil, dead branches, fallen leaves and rotted wood, and fruiting bodies of other fungi are its growth substrates. Species are rich and diverse. Trichoderma is an important natural biological resource with high economic value. Trichoderma harzianum is the most widely used in Trichoderma harzianum, and Trichoderma harzianum is widely used as a biological control agent, and it is widely used in agr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/38A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N3/00
Inventor 刘峰潘明王江伟郭红伟王珍伟
Owner 山东五福生生态工程有限公司
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