Method for producing clostridium butyricum through fermentation of enterococcus faecium fermentation waste liquid

A technology of Clostridium butyricum and fermentation waste liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as polluting the environment, and achieve the effects of reducing pollution, reducing production environmental protection pressure, and reducing costs

Pending Publication Date: 2021-06-15
湖北华扬科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defect of prior art, provide a kind of method that utilizes Enterococcus faecium fermentation waste liquid to ferment and produce Clostridium butyricum, solve the problem that Enterococcus faecium fermentation waste liquid directly discharges and pollute the environment, also reduce simultaneously Clostridium butyricum production cost

Method used

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Examples

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Effect test

Embodiment 1

[0023] The embodiment of the present invention provides a method for producing Clostridium butyricum by fermentation of Enterococcus faecium fermentation waste liquid, comprising the following steps:

[0024] S1. Prepare medium: ferment glucose, corn steep liquor, yeast extract, anhydrous calcium chloride, ferrous sulfate, magnesium sulfate heptahydrate, manganese sulfate monohydrate, dipotassium hydrogen phosphate, sodium acetate trihydrate, and Enterococcus faecium Dissolve the waste liquid in water to configure the medium, including glucose 20g / L, corn steep liquor 25g / L, yeast extract 10g / L, anhydrous calcium chloride 0.45g / L, ferrous sulfate 0.18g / L, magnesium sulfate heptahydrate 0.85g / L, manganese sulfate monohydrate 0.35g / L, dipotassium hydrogen phosphate 4g / L, sodium acetate trihydrate 0.5g / L, Enterococcus faecium fermentation waste liquid 100g / L, sterilize at 115°C for 30min.

[0025] S2. Preparation of Clostridium butyricum seed solution: inoculate Clostridium butyr...

Embodiment 2

[0030] The embodiment of the present invention provides a method for producing Clostridium butyricum by fermentation of Enterococcus faecium fermentation waste liquid, comprising the following steps:

[0031] S1. Prepare medium: ferment glucose, corn steep liquor, yeast extract, anhydrous calcium chloride, ferrous sulfate, magnesium sulfate heptahydrate, manganese sulfate monohydrate, dipotassium hydrogen phosphate, sodium acetate trihydrate, and Enterococcus faecium Dissolve the waste liquid in water to configure the medium, including glucose 40g / L, corn steep liquor 25g / L, yeast extract 10g / L, anhydrous calcium chloride 0.45g / L, ferrous sulfate 0.18g / L, magnesium sulfate heptahydrate 0.85g / L, manganese sulfate monohydrate 0.35g / L, dipotassium hydrogen phosphate 4g / L, sodium acetate trihydrate 0.5g / L, Enterococcus faecium fermentation waste liquid 100g / L, sterilize at 115°C for 30min.

[0032] S2. Preparation of Clostridium butyricum seed solution: inoculate Clostridium butyr...

Embodiment 3

[0037] The embodiment of the present invention provides a method for producing Clostridium butyricum by fermentation of Enterococcus faecium fermentation waste liquid, comprising the following steps:

[0038] The difference between this implementation case and the specific implementation case 1 is that the concentration of the fermentation waste liquid of Enterococcus faecium added in step 1 is 300 g / L, and the other is the same as the specific implementation case 1.

[0039] The number of spores of Clostridium butyricum was 8.6×10 8 CFU / mL.

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Abstract

The invention provides a method for producing clostridium butyricum through fermentation of enterococcus faecium fermentation waste liquid. The method comprises the following steps: S1, preparing a culture medium: dissolving glucose, corn steep liquor, yeast extract, anhydrous calcium chloride, ferrous sulfate, magnesium sulfate heptahydrate, manganese sulfate monohydrate, dipotassium phosphate, sodium acetate trihydrate and enterococcus faecium fermentation waste liquid into water to prepare the culture medium, and performing sterilizing at high temperature; S2, preparing clostridium butyricum seed liquid; S3, performing inoculation: putting the culture medium prepared in the step S1 into a fermentation tank, and inoculating the clostridium butyricum seed liquid prepared in the step S2 into the fermentation tank according to a volume ratio of 3%; and S4, performing culturing: performing culturing under proper conditions to obtain the clostridium butyricum fermentation liquid. According to the method disclosed by the invention, the enterococcus faecium fermentation waste liquid is used as a part of nitrogen source to ferment and produce the clostridium butyricum, so that the waste is utilized, the environmental pollution caused by direct discharge of the enterococcus faecium fermentation waste liquid is reduced, the environmental protection pressure of factory production is reduced, and the production cost is also reduced.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for producing Clostridium butyricum by fermenting waste liquid of Enterococcus faecium. Background technique [0002] Clostridium butyricum is used as a feed additive in feed, and it can embody five biological characteristics in the intestinal tract of animals: First, it promotes the proliferation and development of beneficial bacteria in the intestinal tract of animals (Bifidobacteria, Lactobacillus), inhibits harmful bacteria and The growth and reproduction of spoilage bacteria can correct the disorder of intestinal flora and reduce the occurrence of enterotoxin; the second is that it can produce B vitamins, vitamin K, amylase and other substances in the intestinal tract of animals, which has a health care effect; the third is Clostridium butyricum The main metabolite butyric acid is the main nutrient for the regeneration and repair of intes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/145
CPCC12N1/20
Inventor 李洋张茜茜熊晓燕吴微詹志春周樱辜玲芳刘文悦
Owner 湖北华扬科技发展有限公司
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