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Bacillus subtilis fermenting method with high rate of maturing gemma

A technology of Bacillus subtilis and high spore rate is applied in the field of Bacillus subtilis fermentation and Bacillus subtilis fermentation with high spore rate. The effect of short fermentation period and simple process parameters

Active Publication Date: 2012-11-14
大连吉翔农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the spore rate of Bacillus subtilis fermentation is not high due to the deficiency of existing microbial fermentation technology, and to provide a method for fermentation of Bacillus subtilis with a high spore rate, so as to improve the spore content and stable quality of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] ① Inoculate Bacillus subtilis in glycerin at -70°C with a sterile streak on a sterilized solid medium (the medium is composed of the following components by mass percentage: beef extract 0.30%, sodium chloride 0.50%, peptone 1.00%, agar 2.00%, the balance is water, pH 7.2, 121°C, 0.11Mpa sterilization for 20min), 37°C static culture for 6 days.

[0019] ② Wash the bacterium colony on the solid medium with sterile physiological saline and inoculate it in the sterile medium of a 30L seed fermenter (the medium is composed of the following components by mass percentage: 0.50% sucrose, 0.20% urea, phosphoric acid Dipotassium hydrogen 0.60%, potassium dihydrogen phosphate 0.30%, yeast extract 0.04%, ferric chloride 0.02%, calcium carbonate 0.02%, magnesium sulfate 0.10%, manganese sulfate 0.01%, starch 0.60%, soybean meal 1.25%, soybean 1.00% (crushed after foaming), 0.01% foamed enemy, and the balance is water, pH 6.5, sterilized at 121°C and 0.11Mpa for 25min), and the medi...

Embodiment 2

[0022] ① Inoculate Bacillus subtilis in glycerin at -70°C with a sterile streak on a sterile solid medium (the medium is composed of the following components by mass percentage: beef extract 0.40%, sodium chloride 0.50%, peptone 0.90%, agar 2.00%, the balance is water, pH 7.2, 121°C, 0.11Mpa sterilization for 20min), 37°C static culture for 5 days.

[0023] ② Wash the colony on the solid medium with sterile physiological saline and inoculate it in the medium of a 30L seed fermenter (the medium is composed of the following components in terms of mass percentage: 0.30% sucrose, 0.20% urea, hydrogen phosphate Dipotassium 0.60%, potassium dihydrogen phosphate 0.30%, yeast extract 0.05%, ferric chloride 0.03%, calcium carbonate 0.03%, magnesium sulfate 0.08%, manganese sulfate 0.01%, starch 0.350%, soybean meal 1.00%, soybean 1.20% ( crushed after foaming), soaked in 0.01%, the balance is water, pH 6.5, 121°C, 0.11Mpa sterilization for 25min), medium loading capacity 20L; fermentat...

Embodiment 3

[0026] ① Inoculate Bacillus subtilis in glycerin at -70°C with a sterile streak on a sterile solid medium (the medium is composed of the following components by mass percentage: beef extract 0.30%, sodium chloride 0.50%, peptone 0.80%, agar 2.00%, the balance is water, pH 7.2, 121°C, 0.11Mpa sterilization for 20min), 37°C static culture for 5 days;

[0027]② wash the colony on the solid medium with sterile physiological saline and inoculate it in the medium of a 30L seed fermenter (the medium is composed of the following components in terms of mass percentage: 0.40% sucrose, 0.30% urea, hydrogen phosphate Dipotassium 0.60%, potassium dihydrogen phosphate 0.30%, yeast extract 0.06%, ferric chloride 0.02%, calcium carbonate 0.02%, magnesium sulfate 0.08%, manganese sulfate 0.01%, starch 0.40%, soybean meal 1.20%, soybean 1.00% ( crushed after foaming), soaked in 0.01%, the balance is water, pH 6.8, 121°C, 0.11Mpa sterilization for 25min), medium loading capacity 20L; fermentatio...

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Abstract

The invention discloses a bacillus subtilis fermenting method with a high rate of maturing gemma and belongs to the field of microorganism fermentation engineering. In the method, the target products are produced by activation of bacillus subtilis and two-step fermenting culture. The method comprises the following steps of: inoculating the activated bacillus subtilis first, performing fermentation culture for 8 to 14 hours under the conditions that: the culture temperature is between 35 and 38 DEG C, the cylinder pressure is between 0.02 to 0.06 Mpa, the ventilation rate is 1:0.5-1:1 and the stirring revolution is between 150 and 200 rpm to obtain seed bacterial solution, and inoculating the seed bacterial solution and culturing for 20 to 36 hours to obtain bacillus subtilis liquid products with a high rate of maturing gemma. In the method, the components of the culture medium can be easily obtained and have low price, process parameters are simple, the fermentation period is short, the obtained bacillus subtilis is high in number of living bacteria as well as rate of maturing gemma, the rate of maturing gemma reaches more than 3.0*10<9>CFU / ml, and the number of maturing gemma andthe quality of the products are obviously improved.

Description

technical field [0001] The invention relates to a fermentation method of Bacillus subtilis, in particular to a fermentation method of Bacillus subtilis with a high spore rate, and belongs to the field of microbial fermentation engineering. Background technique [0002] Bacillus subtilis is one of the most widely used enzyme-producing microorganisms. It is widely distributed in soil and decaying organic matter, and it is easy to reproduce in subtilis juice, hence the name. Subtilisin, polymyxin, nystatin, gramicidin and other active substances produced during the growth of Bacillus subtilis, these active substances have obvious effects on pathogenic bacteria or opportunistic pathogenic bacteria of endogenous infection inhibition. Bacillus subtilis can rapidly consume free oxygen in the environment, cause intestinal hypoxia, promote the growth of beneficial anaerobic bacteria, and produce organic acids such as lactic acid, lower the pH value of the intestinal tract, and indir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 崔京春张宝君张献吴俊罡郭海勇
Owner 大连吉翔农业科技有限公司
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