Culture utensil for large-scale cultivation of cordyceps militaris

A technology for Cordyceps militaris and culture vessels, which is applied in specific-purpose bioreactors/fermenters, biochemical instruments, sterilization methods, etc. Hasty and lowly problems, to achieve the effect of reducing labor intensity, reducing the demand for production area, and reducing the infection rate

Active Publication Date: 2010-02-17
唐培宸
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The object of the present invention is to propose a kind of culture vessel that cultivates Cordyceps militaris on a large scale, to solve the problem that the glass round bottles, plastic round bottles and small plastic round boxes used in the tissue culture of Cordyceps militaris cannot supplement the growth and development of Cordyceps militaris because of airtightness The oxygen needed in the process cannot discharge excess water, which leads to the problem of low biotransformation rate and weed rate of Cordyceps

Method used

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  • Culture utensil for large-scale cultivation of cordyceps militaris
  • Culture utensil for large-scale cultivation of cordyceps militaris

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Embodiment 1

[0044] In this embodiment, the culture vessel for tissue culture of Cordyceps militaris is the culture vessel for tissue culture of Cordyceps sinensis described in this specific embodiment. The culture vessel has a height of 100 mm, a length of 290 mm, a width of 190 mm, and a thickness of 5 mm. The ventilation hole is composed of 5 small holes with a diameter of 5mm arranged in a cross shape. Air vent is arranged on the top board of lid apart from the edge 20mm place of top board. Every 250cm of the surface area of ​​the top plate 2 Open a vent. The middle layer of the enclosure is a microporous filter membrane with a diameter of 35 mm and a pore size of 0.45 microns, the surface layer is a layer of pharmaceutical grade non-woven fabric, and the bottom layer is also a layer of pharmaceutical grade non-woven fabric.

[0045] For the selection of tissue culture methods, culture medium, culture solution, and temperature, humidity, light and other condition parameters for cult...

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Abstract

The invention relates to a culture utensil, in particular to a culture utensil for large-scale cultivation of cordyceps militaris. The culture utensil for large-scale cultivation of cordyceps militaris comprises a box body and a box lid which is matched with the box body and can integrally seal the box body. The culture utensil is characterized in that the culture utensil is provided with ventilation holes communicating the inside of the culture utensil with the outside, and a seal body which seals the ventilation holes, only allows air to freely pass in and out and performs microfiltration onthe air is also arranged on the culture utensil. The culture utensil solves the problem that as round glass bottles, round plastic bottles and small round plastic boxes cannot replenish oxygen neededduring the growth and development of the cordyceps militaris or discharge redundant moisture in culture mediums or nutrient solution because of impermeability, the air purification of the cordyceps militaris during growth in the culture utensil cannot be guaranteed, resulting in low cordyceps biotransformation rate and germination rate. The invention further improves the shape and material of theculture utensil.

Description

technical field [0001] The invention relates to a culture vessel, in particular to a culture vessel for tissue culture, in particular to a culture vessel for large-scale cultivation of Cordyceps militaris. Background technique [0002] At present, most scientific research institutes at home and abroad basically use glass round bottles as culture vessels or plastic round bottles of the same specification as culture vessels when conducting biological tissue culture. [0003] In terms of cultivating Cordyceps Militaris, most domestic units currently use glass round bottles as culture vessels, and a few units use plastic round bottles with the same specifications as glass round bottles as culture vessels. [0004] Using the current glass round bottle or plastic round bottle as a culture vessel has the following disadvantages: [0005] 1. The current glass round bottle or plastic round bottle is used as the culture vessel. Due to its small opening and large bottom diameter, it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/22C12M1/12
CPCC12M23/38
Inventor 唐永范唐亮
Owner 唐培宸
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