Method for producing stock seeds of enoki mushrooms

A technology of white Flammulina velutipes and production methods, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of mycelium not easy to decompose, low content of mycelium in strains, high production cost, etc., and achieve easy control of process conditions, The effect of strong vitality and low production cost

Inactive Publication Date: 2013-05-22
辽宁天赢生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The original seed production method in the prior art has high cellulose content in the culture medium, the hyphae are not easy to decompose, and the mycelium content in the strain is low, which is not conducive to the growth of the seed material surface after inoculation, and the uneven stirring affects the growth of the original seed Consistency, resulting in easy degradation of strains, uneven output of cultivation bottles; using this method, the growth rate of bacteria is slow, the production cost is high, and the yield and properties of the product are not stable enough

Method used

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  • Method for producing stock seeds of enoki mushrooms
  • Method for producing stock seeds of enoki mushrooms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) The weight percent of each raw material in the culture medium is: sawdust 60%, rice bran 34%, cottonseed hull 5%, light calcium carbonate 1%;

[0018] 2) Material preparation: The wood chips used are poplar and willow broad-leaved wood chips. During the stacking process of the wood chips, water should be added to the stack for 1-2 months, and the stack should be turned over and added with water for 1-2 months. Once, water should be replenished each time the pile is turned, so that the water content of the sawdust pile is controlled at 60-65wt%; sieve before use, and the particle size of the sawdust is 2-4mm;

[0019] Before preparing materials, use an infrared moisture analyzer to measure the water content of wood chips and rice bran (the amount of cottonseed hulls and light calcium carbonate is small, and the water content is negligible), and calculate the amount of raw materials added according to the water content; first add wood chips to the stirring pot, Then ad...

Embodiment 2

[0027] 1) The percentage by weight of each raw material in the culture medium is: wood chips 50%, rice bran 38%, cottonseed hulls 10%, light calcium carbonate 2%;

[0028] 2) Material preparation: The wood chips used are poplar, willow and elm broad-leaved wood chips. During the stacking process of the wood chips, water should be added to the stack for 1-2 months, and the stack should be turned over and then watered for 1-2 months. Turn the pile at least twice, and replenish water for each turn, so that the water content of the sawdust pile is controlled at 60-65wt%; sieve before use, and the particle size of the sawdust is 2-4mm;

[0029] Before preparing materials, use an infrared moisture analyzer to measure the water content of wood chips and rice bran, and calculate the amount of raw materials added according to the water content; first add wood chips to the stirring pot, then add rice bran, cottonseed hulls and light calcium carbonate and stir for 20 minutes to make the c...

Embodiment 3

[0037] 1) The weight percent of each raw material in the culture medium is: sawdust 56%, rice bran 35%, cottonseed hull 8%, light calcium carbonate 1%;

[0038] 2) Material preparation: The wood chips used are poplar and willow broad-leaved wood chips. During the stacking process of the wood chips, water should be added to the stack for 1-2 months, and the stack should be turned over and added with water for 1-2 months. Once, water should be replenished each time the pile is turned, so that the water content of the sawdust pile is controlled at 60-65wt%; sieve before use, and the particle size of the sawdust is 2-4mm;

[0039] Before preparing materials, use an infrared moisture analyzer to measure the water content of wood chips and rice bran, and calculate the amount of raw materials added according to the water content; first add wood chips to the stirring pot, then add rice bran, cottonseed hulls and light calcium carbonate and stir for 20 minutes to make the culture medium...

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Abstract

The invention discloses a method for producing stock seeds of enoki mushrooms. The method includes preparing culture media comprising 50-60% of sawdust, 34-45% of rice bran, 4-10% of cottonseed hulls and 0.5-2% of light calcium carbonate and enabling the moisture content of the culture media to be 62-64%; bottling, coring and covering the culture media in an environment with the room temperature lower than 30 DEG C, sterilizing the culture media at the temperature of 110+/-2 DEG C under the pressure of 0.1-0.12Mpa for 60 minutes, keeping the culture media at the temperature of 121+/-2 DEG C under the pressure of 0.12-0.13Mpa for 60-90 minutes, and feeding sterilized strain bottles into a cooling chamber to realize forced cooling for the strain bottles until the temperature of each strain bottle ranges from 16 DEG C to 20 DEG C; selecting second-level strains, inoculating the stock seeds of the second-level strains via a solid strain machine; culturing the stock seeds under conditions that the temperature ranges from 17 DEG C 20 DEG C, the humidity ranges from 65% to 70% and the concentration of carbon dioxide ranges from 1000ppm to 2000ppm after the stock seeds are inoculated; and selecting the stock seeds thrice in a culture process and culturing the stock seeds for 16-19 days before the stock seeds can be used. The inoculation amount of the stock seeds is proper so that a material surface can be covered. The method has the advantages that mycelia of the stock seeds are healthy, strong, white and vigorous, and are high in activity, and the strains are not easy to degenerate and are short in growth cycle; the enoki mushrooms can germinate quickly after inoculation and are low in contamination rate and high in yield; and the manufacturing cost is low, and technological conditions are easy to control.

Description

technical field [0001] The invention relates to a production method of edible fungus, in particular to a production method of the original species of Flammulina velutipes. Background technique [0002] White Flammulina velutipes is a white variant (F.var.velutipes) of Flammulina velutipes, scientific name is Enoki Mushroom, and Latin literary name is Flammulina velutiper (Fr.) Sing. Bacteriaceae, Tricholomaceae, Flammulina or Collybia. [0003] Flammulina velutipes is cultivated on a large scale in Japan, South Korea, China, and Taiwan, China. Domestically, it is mainly imported and domesticated from Japan for factory cultivation and production. The preparation method of Flammulina velutipes original species (secondary species) is mainly solid original species and liquid strains , the existing preparation method of solid seed in my country mainly uses sawdust, bran, sugar as nutrient source, wherein 70-80% of broad-leaved sawdust, 20-30% of bran, 1-3% of sugar, and 1% of gyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 刘汉席
Owner 辽宁天赢生物科技股份有限公司
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