Transparent in-situ three-dimensional plant cultivation device and application thereof in plant root system observation

Abstract

The invention discloses a transparent in-situ three-dimensional plant cultivation device and application thereof in plant root system observation. The outside of the device is a transparent cylinder,and the top of a container is sealed by a breathable film; a transparent gel culture medium is contained in the container; a certain space is formed between the transparent gel culture medium and thetop breathable film; the transparent gel culture medium is divided into an upper layer and a lower layer, a layered cultivation mode is adopted, the upper layer has an antibacterial effect, and PPM with the concentration range of 0.005-0.05% in volume percentage concentration which has small influence on root system growth under the condition of ensuring the antibacterial effect is selected, so that the contamination rate of the whole culture medium is inhibited while the root growth is not influenced, the definition of in-situ root system observation can be obviously improved, and ideal results are obtained for dynamic real-time observation and related functional characters obtained after plants are harvested.

Description

Technical field [0001] The invention belongs to plant root ecology, and relates to a transparent in-situ three-dimensional plant cultivation device and its application in plant root system observation. Background technique [0002] The root system has received extensive attention in recent years because of its important role in anchoring plants and nutrient absorption. Due to the opacity of root growth underground in the field environment, in-situ root observation is very difficult. In-situ observation of the root system includes the use of X-ray plane tomography and MRI (Magnetic Resonance Imaging, MRI) and other penetrating radiation methods, the use of observation methods placed in the soil of the root box or micro-root canal, and the transparent soil composed of sulfonic acids Medium observation method, as well as medium optical imaging observation method, etc. Although the first three types of methods have high accuracy, they require more expensive instruments and material...

AI Technical Summary

Problems solved by technology

However, because the existing transparent gel technology seldom considers the requirements for imaging clarity, and the conventional surface disinfection steps of seeds and explants cannot remove the endophytic fungi that are often contained in plant seeds, making the inside of the medium prone to contamination after planting. The status of the bacteria, which has a great impact on the clarity of our in situ observation of the root system in the gel medium, and because the gel is easily contaminated, the success rate of the final successful image acquisition is reduced

[0003] At present, the existing plant cultivation system, two-dimensional paper cultivation method and bag cultivation method have a serious impact on the three-dimensional configuration of plants, and are more suitable for the observation of the early root morphology of herbaceous plants; transparent soil composed of sulfonic acid substances requires optical tomography As well as confocal technology, the cost is relatively high; due to the high sterilization requirements of the transparent gel medium, once the operation is not standardized, it is easy to cause the medium to be contaminated with bacteria and affect the imaging of the root system. Bacteria can be effectively suppressed broad-spectrum, but it will also affect the growth and development of the root system to a certain extent and even cause it to die

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