Tissue culture medium of phalaenopsis and propagation method of phalaenopsis
A tissue culture medium and phalaenopsis technology, applied in the field of plant tissue culture, can solve the problems of low reproductive coefficient of adventitious buds, affecting proliferation efficiency and quality of seedlings, etc. Effect
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[0025] Embodiment 1-3 result
[0026]
[0027] 3) Proliferation culture of tissue cultured seedlings: Cut off the leaves and bases of the induced buds and inoculate them into the proliferation medium, at 25-28°C, light intensity 1500-2000Lx, light 12h / d, the multiplication factor> 5;
[0028] Described proliferation medium is 1 / 2MS+6-BA 3.5mg / L+KT1.0mg / L+NAA0.4mg / L+ albumen age 2g / L+coconut juice 100mL / L+ white sugar 20g / L, agar 5g / L, pH=5.5-5.6;
[0029]
[0030] 4) Cultivation of strong seedlings: Cut the proliferated buds and transfer them to the medium for strong seedlings. The culture temperature is 23-27°C, the light is 1500-2000Lx, the light is 10-12h per day, and the culture is 5-10 days;
[0031] The medium for strong seedlings is MS+6-BA2mg / L+protein age 2g / L+coconut juice 100mL / L+sugar 30g / L+agar 5g / L, pH=5.5~5.6;
[0032] 5) Rooting culture: Inoculate the Phalaenopsis seedlings cultivated by strong seedlings into the rooting medium, cultivate at 23-27°C, li...
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