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Browning-prevention Phalaenopsis amabilis tissue culture method and browning-prevention culture medium

A technology of culture medium and browning group, which is applied in the field of plant tissue culture, can solve the problems of not being environmentally friendly, few anti-browning methods, and incompleteness, and achieve the effects of cost saving, low browning rate, and healthy nutrition

Inactive Publication Date: 2014-04-30
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not difficult to find that these methods have certain defects and incompleteness and are not environmentally friendly. There are still very few studies on the anti-browning of the entire cultivation stage and a relatively systematic anti-browning method.

Method used

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  • Browning-prevention Phalaenopsis amabilis tissue culture method and browning-prevention culture medium
  • Browning-prevention Phalaenopsis amabilis tissue culture method and browning-prevention culture medium
  • Browning-prevention Phalaenopsis amabilis tissue culture method and browning-prevention culture medium

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Embodiment Construction

[0039] The technical solutions of the present invention will be further described below through specific examples.

[0040] Following example MS culture medium formula: Contain KNO in every liter of culture medium 3 (1900mg / L), NH 4 NO 3 (1650mg / L), MgSO 4 ·7H 2 O (370mg / L), KH 2 PO 4 (170mg / L), CaCl 2 (330mg / L), KI (0.83mg / L), H 3 BO 3 (6.2mg / L), MnSO 4 ·H 2 O (16.9mg / L), ZnSO 4 ·7H 2 O (8.6mg / L), CuSO 4 ·5H 2 O (0.025mg / L), CoCl 2 ·6H 2 O (0.025mg / L), Na 2 MoO 4 2H 2 O (0.25mg / L), FeSO 4 ·7H 2 O (27.85 mg / L), Na 2 EDTA (37.25mg / L), glycine (2.0mg / L), niacin B3 (0.5mg / L), thiamine hydrochloride B1 (0.1mg / L), pyridoxine hydrochloride B6 (0.5mg / L), Inositol (100mg / L).

[0041]B5 medium formula: KNO 3 (2500mg / L), (NH 4 ) 2 SO 4 (134mg / L), NaH 2 PO 4 ·H 2 O (150mg / L), MgSO 4 ·7H 2 O (250mg / L), CaCl 2 (124mg / L), KI (0.75mg / L), H 3 BO 3 (3mg / L), MnSO 4 ·H 2 O (10mg / L), ZnSO 4 ·7H 2 O (2mg / L), CuSO 4 ·5H 2 O (0.025mg / L), CoCl 2 ·6H 2 O (0.0...

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Abstract

The invention relates to a combined method for preventing Phalaenopsis amabilis from browning in plant tissue culture, the adopted V31 (Phalaenopsis amabilis) is also called 'TaiLinhongtianshi', and is one of a red flower line of the Phalaenopsis amabilis. A culture medium containing natural additives is utilized for preventing the common browning phenomenon of the Phalaenopsis amabilis, so as to form the combined browning-prevention method. The method has high stability and high success rate, is green and environment-friendly, can be repeatedly used for production annually, and solves the problem that the existing method has low success rate and is not environment-friendly, thereby having great significance of real-world applications.

Description

technical field [0001] The invention mainly relates to the field of plant tissue culture, in particular to a moth orchid anti-browning tissue culture method and an anti-browning culture medium. Background technique [0002] Browning of explants is an important problem encountered in plant tissue culture. Studies have shown that the main cause of browning is caused by polyphenol oxidase acting on natural substrate phenolic substances to form quinones. The browning phenomenon can be better controlled by adding antioxidants and adsorbents. [0003] In response to these causes of browning, earlier studies have shown that activated carbon can prevent browning, because the principle of activated carbon anti-browning is the adsorption of activated carbon, and the adsorption of activated carbon itself is limited, that is, It is impossible to add a large amount of activated carbon to each bottle, so the adsorption effect of a small amount of activated carbon is relatively small. Onc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 项艳赵康沈周高任洁冯琳赵华琳
Owner ANHUI AGRICULTURAL UNIVERSITY
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