Phalaenopsis amabilis anti-browning tissue culture method taking lemon extract as anti-browning culture medium
A technology of lemon extract and culture medium, which is applied in the field of plant tissue culture, can solve the problems of environmental protection, incompleteness, and few anti-browning methods, and achieve the effects of cost saving, nutrition and health, and low browning rate
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[0040] The technical solutions of the present invention will be further described below through specific examples.
[0041] Following example MS culture medium formula: Contain KNO in every liter of culture medium 3 (1900mg / L), NH 4 NO 3 (1650mg / L), MgSO 4 ·7H 2 O (370mg / L), KH 2 PO 4 (170mg / L), CaCl 2 (330mg / L), KI (0.83mg / L), H 3 BO 3 (6.2mg / L), MnSO 4 ·H 2 O (16.9mg / L), ZnSO 4 ·7H 2 O (8.6mg / L), CuSO 4 ·5H 2 O (0.025mg / L), CoCl 2 ·6H 2 O (0.025mg / L), Na 2 MoO 4 2H 2 O (0.25mg / L), FeSO 4 ·7H 2 O (27.85mg / L), Na 2EDTA (37.25mg / L), glycine (2.0mg / L), niacin B3 (0.5mg / L), thiamine hydrochloride B1 (0.1mg / L), pyridoxine hydrochloride B6 (0.5mg / L), Inositol (100mg / L).
[0042] B5 medium formula: KNO 3 (2500mg / L), (NH 4 ) 2 SO 4 (134mg / L), NaH 2 PO 4 ·H 2 O (150mg / L), MgSO 4 ·7H 2 O (250mg / L), CaCl 2 (124mg / L), KI (0.75mg / L), H 3 BO 3 (3mg / L), MnSO 4 ·H 2 O (10mg / L), ZnSO 4 ·7H 2 O (2mg / L), CuSO 4 ·5H 2 O (0.025mg / L), CoCl 2 ·6H 2 O (0.02...
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