A kind of Phalaenopsis anti-browning tissue culture method using lemon extract as anti-browning medium

A technology of lemon extract and culture medium, applied in the field of plant tissue culture, can solve the problems of few anti-browning methods, unenvironmental protection, incompleteness and the like, and achieve the effects of low browning rate, cost saving, and nutritional health.

Inactive Publication Date: 2016-09-14
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not difficult to find that these methods have certain defects and incompleteness and are not environmentally friendly. There are still very few studies on the anti-browning of the entire cultivation stage and a relatively systematic anti-browning method.

Method used

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  • A kind of Phalaenopsis anti-browning tissue culture method using lemon extract as anti-browning medium
  • A kind of Phalaenopsis anti-browning tissue culture method using lemon extract as anti-browning medium
  • A kind of Phalaenopsis anti-browning tissue culture method using lemon extract as anti-browning medium

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Embodiment Construction

[0040] The technical solutions of the present invention will be further described below through specific examples.

[0041] Following example MS culture medium formula: Contain KNO in every liter of culture medium 3 (1900mg / L), NH 4 NO 3 (1650mg / L), MgSO 4 ·7H 2 O (370mg / L), KH 2 PO 4 (170mg / L), CaCl 2 (330mg / L), KI (0.83mg / L), H 3 BO 3 (6.2mg / L), MnSO 4 ·H 2 O (16.9mg / L), ZnSO 4 ·7H 2 O (8.6mg / L), CuSO 4 ·5H 2 O (0.025mg / L), CoCl 2 ·6H 2 O (0.025mg / L), Na 2 MoO 4 2H 2 O (0.25mg / L), FeSO 4 ·7H 2 O (27.85mg / L), Na 2EDTA (37.25mg / L), glycine (2.0mg / L), niacin B3 (0.5mg / L), thiamine hydrochloride B1 (0.1mg / L), pyridoxine hydrochloride B6 (0.5mg / L), Inositol (100mg / L).

[0042] B5 medium formula: KNO 3 (2500mg / L), (NH 4 ) 2 SO 4 (134mg / L), NaH 2 PO 4 ·H 2 O (150mg / L), MgSO 4 ·7H 2 O (250mg / L), CaCl 2 (124mg / L), KI (0.75mg / L), H 3 BO 3 (3mg / L), MnSO 4 ·H 2 O (10mg / L), ZnSO 4 ·7H 2 O (2mg / L), CuSO 4 ·5H 2 O (0.025mg / L), CoCl 2 ·6H 2 O (0.02...

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Abstract

The invention discloses a phalaenopsis amabilis anti-browning tissue culture method taking a lemon extract as an anti-browning culture medium. The culture medium comprises a primary culture medium and a proliferation and differentiation culture medium, wherein each of the primary culture medium and the proliferation and differentiation culture medium respectively contains a certain volume of natural lemon extract. The tissue culture method disclosed by the invention comprises the steps of carrying out explant disinfection, carrying out primary culture, carrying out proliferation and differentiation culture, carrying out rooting culture, exercising seedlings, transplanting and the like. According to the tissue culture method, the culture medium containing natural additives is used for playing a role in preventing and treating the common browning problem of phalaenopsis amabilis, and meanwhile, the browning problem of a differentiation stage is prevented and treated in an assisted manner by adopting different cutting manners, so that a combined anti-browning method is formed. The method disclosed by the invention is high in stability and high in success rate, is environment-friendly and can be applied to repeated production annually; the problems of low success rate and no environment-friendliness in the existing methods are solved, so that the method has great practical application significance.

Description

[0001] This application is the application number: 201410003340X, the application date is: 2013-4-27, and the invention name is: a divisional application of a Phalaenopsis anti-browning tissue culture method and anti-browning medium technical field [0002] The invention mainly relates to the field of plant tissue culture, in particular to a moth orchid anti-browning tissue culture method and an anti-browning culture medium. Background technique [0003] Browning of explants is an important problem encountered in plant tissue culture. Studies have shown that the main cause of browning is caused by polyphenol oxidase acting on natural substrate phenolic substances to form quinones. The browning phenomenon can be better controlled by adding antioxidants and adsorbents. [0004] In response to these causes of browning, earlier studies have shown that activated carbon can prevent browning, because the principle of activated carbon anti-browning is the adsorption of activated car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 项艳赵康任洁沈周高冯琳赵华琳
Owner ANHUI AGRICULTURAL UNIVERSITY
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