A kind of clematis tissue culture medium and its preparation method and application
A culture medium and tissue culture technology, applied in the field of tissue culture culture medium and preparation of A. Good sterilization effect
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Embodiment 1
[0020] In the embodiment of the present invention, a clematis tissue culture medium is added with 5 g of agar, 20 g of Eclipta extract, 15 g of curculigo extract, 12 g of mother-of-pearl, and 3 g of kaolin per liter of MS medium.
[0021] Crush Eclipta, pass through a 80-mesh sieve, add 5 times its mass of citric acid aqueous solution to extract twice, the extraction time is 3.8h, the extraction temperature is 72°C, and the mass content of the citric acid aqueous solution is 3 %, the combined extracts were concentrated to 15% of the original volume to obtain the Eclipta chinensis extract. Crush curculigo, pass through a 100-mesh sieve, add deionized water with 11 times its mass, decoct with strong fire for 1 hour, stop heating, add absolute ethanol with 0.5 times the mass of curculigo, soak for 1.5 hours, then decoct with slow fire for 1 hour, Take the filtrate by filtration and concentrate to 15% of the original volume to obtain Curculigo curculigo extract. Crush mother-of-p...
Embodiment 2
[0023] In the embodiment of the present invention, a clematis tissue culture medium is added with 5 g of agar, 28 g of Eclipta extract, 23 g of curculigo extract, 20 g of mother-of-pearl, and 10 g of kaolin per liter of MS medium.
[0024] Crush Eclipta, pass through a 80-mesh sieve, add 6 times its mass of citric acid aqueous solution for leaching twice, the leaching time is 4 hours, the leaching temperature is 75°C, and the mass content of the citric acid aqueous solution in citric acid is 4%. , combine the extracts, and concentrate to 18% of the original volume to obtain the extract of Eclipta. Crush curculigo, pass through a 100-mesh sieve, add deionized water with 11 times its mass, decoct with strong fire for 1 hour, stop heating, add absolute ethanol with 0.8 times the mass of curculigo, soak for 2 hours, then decoct with slow fire for 1.5 hours, Take the filtrate by filtration and concentrate to 20% of the original volume to obtain Curculigo curculigo extract. Crush m...
Embodiment 3
[0026] In the embodiment of the present invention, a clematis tissue culture medium is added with 5 g of agar, 22 g of Eclipta extract, 17 g of curculigo extract, 14 g of mother-of-pearl, and 5 g of kaolin per liter of MS medium.
[0027] Grind Eclipta grass, pass through a 80-mesh sieve, add 5.5 times the mass of citric acid aqueous solution to extract twice, the extraction time is 3.9 hours, the extraction temperature is 73 ° C, and the mass content of the citric acid aqueous solution in citric acid is 3.5 %, the combined extracts were concentrated to 16% of the original volume to obtain the extract of Eclipta. Crush curculigo, pass through a 100-mesh sieve, add 11 times the mass of deionized water, decoct with strong fire for 1 hour, stop heating, add absolute ethanol with 0.6 times the mass of curculigo, soak for 1.8 hours, then simmer for 1.2 hours , filtered to take the filtrate, and concentrated to 18% of the original volume to obtain the curculigo extract. Crush mothe...
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