The present invention relates to a laundry sheet, which comprises, by weight, 6-8 parts of polyvinyl alcohol, 3-5 parts of cross-linked sodium hydroxymethyl starch, 2-3 parts of cross-linked polyvinylpyrrolidone, 1-5 parts of sucrose ester, 3-5 parts of cocoacyl hydroxyethyl sulfonate sodium, 3-5 parts of polyoxyethylene lauryl ether, 1-2 parts of cocoamidopropyl dimethyl betaine, 1-5 parts of dodecyl dimethyl sulfopropyl betaine, 1-3 parts of chitosan, 2-5 parts of malic acid, 1-3 parts of an acrylic acid-2-acrylamide-2-methyl propane sulfonic acid-hydroxypropyl acrylate terpolymer, 2-5 parts of dipotassium glycyrrhizinate, 1-2 parts of MA5-DETA, 2-3 parts of decanoyl acetaldehyde, 2-3 parts of methyl nonyl ketone lauric acid, an orange peel extraction liquid, a pomegranate peel extraction liquid, bamboo vinegar, sodium chloride, polyol, enzyme, and deionized water. The invention further relates to a preparation method of the laundry sheet. The laundry sheet of the present invention has characteristics of small size, easy storage, cleaning, easy carrying, and environmental protection.