Method for comprehensively using viscera of sea cucumber

A technology for sea cucumber viscera and materials, applied in the direction of fermentation, can solve the problems of low safety, difficult industrial production, complex process, etc., and achieve the effects of high safety, scientific design and scientific process route.

Inactive Publication Date: 2012-07-25
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of the above methods is relatively complicated, the extraction rate is low, and it is difficult to implement large-scale industrial production, and the organic solvent extraction method will cause solvent residues in the product, and the safety is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step (1) take 10 kg of sea cucumber viscera and thaw, wash, pulverize and homogenate;

[0029] Step (2) Add 10kg of water, adjust the pH value to 7.8, heat up to 48°C, and carry out alkaline extraction for 210 minutes;

[0030] Step (3) Add 80 g of alkaline protease, adjust the pH value to 8.2, heat up to 55° C., and perform enzymatic hydrolysis for 120 minutes;

[0031] Step (4) centrifuging the material after enzymolysis to obtain supernatant and filter residue respectively;

[0032] In step (5), the supernatant obtained by centrifugation is removed by a microfiltration membrane device to obtain a microfiltration membrane filtrate. The pore size of the microfiltration membrane is 0.2um. ~50℃, the flow rate is 65L / min;

[0033] Step (6) Concentrating the microfiltration membrane filtrate with a nanofiltration membrane device to obtain a nanofiltration membrane concentrate, the nanofiltration membrane filter aperture is 0.5nm, and the working conditions of the nanofil...

Embodiment 2

[0038] Step (1) take 10 kg of sea cucumber viscera and thaw, wash, pulverize and homogenate;

[0039] Step (2) Add 20kg of water, adjust the pH value to 8.2, heat up to 52°C, and carry out alkaline extraction for 270 minutes;

[0040] Step (3) Add 120 g of alkaline protease, adjust the pH value to 8.8, heat up to 65° C., and perform enzymatic hydrolysis for 150 minutes;

[0041] Step (4) centrifuging the material after enzymolysis to obtain supernatant and filter residue respectively;

[0042] In step (5), the supernatant liquid obtained by centrifugation is removed by a microfiltration membrane device to obtain a microfiltration membrane filtrate. The pore size of the microfiltration membrane is 0.5um. ~50℃, the flow rate is 68L / min;

[0043] Step (6) Concentrating the microfiltration membrane filtrate with a nanofiltration membrane device to obtain a nanofiltration membrane concentrate, the nanofiltration membrane filter aperture is 0.2nm, and the working conditions of the...

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PUM

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Abstract

The invention relates to a method for comprehensively using viscera of a sea cucumber, particularly relates to a method for extracting sea cucumber polysaccharide and sea cucumber polypeptide from the viscera of the sea cucumber through a biological enzymolysis technology and a membrane separation technology. The method comprises the following main steps of: 1, pre-treatment; 2, alkali extraction; 3, enzymolysis, 4, centrifugal separation; 5, microfiltration membrane impurity removal; 6, nonefiltration membrane concentration; 7, ethanol precipitation; 8, centrifugal separation; and 9, drying for obtaining sea cucumber polysaccharide and sea cucumber polypeptide respectively. The technical scheme provided by the invention is simple and easy in process and is applicable to industrial production. The extraction rate of protein and total sugar which are contained in viscera of sea cucumber can reach 60-70%. The effective active ingredients in the target products have higher contents. Furthermore, no residual solvent exists, and the security is high.

Description

technical field [0001] The invention relates to a method for comprehensive utilization of sea cucumber viscera, and mainly relates to a method for extracting sea cucumber polysaccharides and sea cucumber polypeptides by using sea cucumber viscera as raw materials and adopting biological enzymolysis technology and membrane separation technology. Background technique [0002] Sea cucumber is a kind of mollusk living in the ocean. It is not only a traditional precious food, but also a valuable medicinal material. Sea cucumbers mainly contain a variety of active and effective ingredients, such as sea cucumber polysaccharides, sea cucumber proteins, sea cucumber saponins, etc., which have high nutritional and medicinal values. [0003] Sea cucumber polysaccharides have a variety of physiological activities, the main performances are: anti-tumor; anti-coagulation; improving immunity; delaying aging; lowering blood fat; beautifying; [0004] Sea cucumber polypeptide has the charac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C08B37/00
Inventor 万端极徐国念吴正奇李猷万莹吴莎肖诗英刘玲海文英王雄司佳吴尧杨涛马超徐礼鹏廖宇杰邹华蓉蔡君
Owner 武汉普赛特膜技术循环利用有限公司
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