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Opportunity and method for inducing 2n pollen of phalaenopsis amabilis

A technology for phalaenopsis and pollen, applied in the field of polyploid breeding, can solve the problems of complex ploidy and genetic background, unable to obtain ideal 2n pollen induction rate, and increasing difficulty in 2n pollen induction research.

Active Publication Date: 2021-11-02
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the prior art, there is little use of 2n pollen induction for Phalaenopsis to obtain doubled plants, and the ideal 2n pollen induction rate cannot be obtained
In addition, there are many varieties of Phalaenopsis, and the ploidy and genetic background are complex, which increases the difficulty of research on 2n pollen induction.

Method used

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  • Opportunity and method for inducing 2n pollen of phalaenopsis amabilis
  • Opportunity and method for inducing 2n pollen of phalaenopsis amabilis
  • Opportunity and method for inducing 2n pollen of phalaenopsis amabilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: Natural 2n pollen yield statistics

[0086] After the Phalaenopsis pollen matures, it exists in the form of tetrads. Observe and record the number of dyads, triads, and tetrads in the mature pollen of Phalaenopsis, and calculate the natural 2n pollen rate. Each Phalaenopsis variety randomly divides 3- 5 pollen blocks were placed on a glass slide, crushed with tweezers, and three visual fields were randomly counted, repeated 3 times. 2n pollen ratio=(2Dy+Tr) / (2Dy+3Tr+4Te), where Dy is the number of dyads, Tr is the number of triads, and Te is the number of tetrads.

[0087] It was found that, except Mantianhong ( image 3 E) Except that normal pollen cannot be produced due to synaptonemal disorder, dyads and triads have been observed in the other five species of Phalaenopsis ( image 3 A-D, image 3 F). The statistics of the natural 2n pollen rates of five species of Phalaenopsis are shown in Table 1. It is found that the natural 2n pollen rates are sig...

Embodiment 2

[0092] Example 2: Using colchicine to induce 2n pollen

[0093] Divide the absorbent cotton into small pieces of equal size and side length about 2 cm, and immerse them in the prepared colchicine solution for later use, the concentrations are 0.01%, 0.05%, and 0.1%, respectively. Take photos at around 10:00 in the morning to record the diameter of the flower buds on the flower stalks to be treated, and then wrap the absorbent cotton with different concentrations of colchicine solution on the flower buds for 3 days, and treat 6-7 flower stalks for each concentration, and each flower stalk is not lower In four flower buds, the effects of meiosis stage and colchicine concentration on the 2n pollen induction rate of Phalaenopsis were mainly investigated. When the buds reached the mature pollen state, the buds were collected, and the numbers of dyads, triads and tetrads were counted in the same manner as in Example 1, and the 2n pollen rate was calculated.

[0094] The flower buds...

Embodiment 3

[0105] Embodiment 3: establish the corresponding relationship between Phalaenopsis flower bud diameter and pollen meiosis stage

[0106] From the end of May to the end of August 2020, randomly select three smaller flower buds of each variety to observe the growth changes of the flower buds. At around 10:00 in the morning, take pictures to record the diameter of the flower buds, and record once a day until the flower buds bloom.

[0107] At the same time, 6 kinds of Phalaenopsis flower buds of different sizes were picked at 10:00-14:00 on a sunny day (10-20 flower buds of different sizes were picked for each kind, repeated 3 times), and the diameter of the flower buds was recorded by taking pictures, and put into Carnot’s fixative ( Absolute ethanol: glacial acetic acid = 3:1) fixed for 12-24h, then transferred to 70% ethanol, and stored in a refrigerator at 4°C for later use. After washing with distilled water for 2-3 times, place the anthers on the glass slide, mash them with t...

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Abstract

The invention discloses a method for determining the opportunity of inducing 2n pollen of phalaenopsis amabilis by colchicine. The method comprises the following steps of (a) counting the yield of natural 2n pollen; (b) conducting 2n pollen induction: selecting the pollen in each meiosis period, and treating flower buds with 0.01%-0.1% colchicine solution to perform 2n pollen induction; (c) counting the 2n pollen rate after the induction; and (d) comparing the 2n pollen rate obtained after induction with the natural 2n pollen rate so as to determine the opportunity for inducing the 2n pollen. The invention also provides a method for inducing 2n pollen of phalaenopsis amabilis by using the colchicine. According to the method, the opportunity for inducing the 2n pollen by utilizing the colchicine and the corresponding colchicine concentration are determined, and a foundation is laid for obtaining the 2n pollen through polyploid induction and cultivating a new phalaenopsis variety which is large in flower size, bright in color and high in ornamental value.

Description

technical field [0001] The invention belongs to the technical field of polyploid breeding, and in particular relates to the timing and induction method of colchicine inducing Phalaenopsis to produce 2n pollen. Background technique [0002] Phalaenopsis is one of the most important economic crops in the world's potted and cut flower markets. Its flowers are shaped like butterflies and have bright and rich colors. It has the reputation of "Queen of Orchids". Polyploid plants usually have larger organs and stronger adaptability under adverse conditions, and polyploid orchids usually have the advantages of strong plants, bright colors, and strong stress resistance. In fact, most of the popular Phalaenopsis varieties on the market are polyploid. The number of chromosomes of the native species of Phalaenopsis is 2n=2x=38, while most of the chromosomes of cultivated species are not fixed, mainly tetraploid and triploid and aneuploidy. Zhuang Donghong et al. (Zhuang Donghong et al...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 贾瑞冬吴婷葛红杨树华赵鑫寇亚平李秋香
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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