Dog parvovirus attenuated vaccine strain and application thereof

A technology of canine parvovirus and attenuated vaccine, which is applied in the field of preparing drugs for preventing or treating diseases caused by canine parvovirus and virus attenuated vaccine strains, can solve the problems of high cost and weak antigen targeting, and achieves good effects, Prevention or treatment of canine distemper, good protective effect

Inactive Publication Date: 2011-01-12
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the above vaccines have disadvantages such as high cost and...

Method used

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  • Dog parvovirus attenuated vaccine strain and application thereof
  • Dog parvovirus attenuated vaccine strain and application thereof
  • Dog parvovirus attenuated vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Isolation and identification of canine parvovirus virulent isolate (CPV-YN)

[0013] 1 Materials and methods

[0014] 1.1 Materials

[0015] 1.1.1 Disease material The diseased and dead dogs of Yinong Comprehensive Farm in Daowai District, Harbin City.

[0016] 1.1.2 CRFK cells (65-95 generations the same below) were purchased from China Veterinary Drug Control Institute and cultured according to conventional methods.

[0017] 1.1.3 As experimental animals, puppies aged 2 to 4 months (CPV antibody titer ≤ 1:2) were purchased from the Department of Experimental Zoology of Harbin Medical University.

[0018] 1.1.4 Serum CPV standard positive serum (antibody titer ≥ 1:16) and CPV negative serum were provided by Harbin Veterinary Research Institute.

[0019] 1.2 Separation method

[0020] 1.2.1 Isolation of virus 5 samples of bloody feces from sick dogs were prepared into a 1:9 suspension with MEM cell nutrient solution, mixed evenly, centrifuged at 5000r / min ...

Embodiment 2

[0066] Embodiment 2 The cultivation of canine parvovirus attenuated vaccine strain CPV-YNR

[0067] 1.1 Test material

[0068] The test animals are healthy dogs (CPV antibody titer ≤ 1:2) of 2 to 4 months of age; anti-canine parvovirus-specific serum and 0.2% erythrocyte suspension are prepared by our laboratory, and the virulent is isolated from Example 1. CPV-YN 6 generations.

[0069] 1.2 Cultivation and identification of CPV-YN attenuated strain

[0070] 1.2.1 Cultivation of CPV-YN attenuated strain Passage CPV-YN to 110 generations through CRFK cells.

[0071] 1.2.2 Morphological observation of attenuated CPV-YN strain

[0072] Centrifuge the harvested CPV-YN 5, 10, 20, 30, 50, 70, 90, 100, 105, and 110 passage virus cell cultures at 5000r / min for 30min, take the supernatant and ultracentrifuge at 20000rpm, and centrifuge at 4°C for 2h. Suspend the precipitate with an appropriate amount of PBS (pH 7.0), and observe with a negative staining electron microscope with pho...

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Abstract

The invention discloses a dog parvovirus attenuated vaccine strain and application thereof. In the invention, the separated dog parvovirus virulent strain is cultivated in a cell subculture mode to obtain the dog parvovirus attenuated vaccine strain. The microbial preservation number is CGMCC No.3841. The test on safety and immunogenicity indicates that the attenuated vaccine strain CPV-YNR can protect the dog subjected to the attack of homologous virulent strain and has favorable immunogenicity. The attenuated vaccine strain CPV-YNR can be used for preparing a single vaccine or combined vaccine, and can effectively prevent or treat diseases caused by dog parvovirus. The attenuated vaccine strain CPV-YNR has the advantages of stable heritability, enduring immunity, favorable effect, high safety and reliability, long storage time and the like.

Description

technical field [0001] The present invention relates to a virus attenuated vaccine strain, especially relates to an attenuated vaccine strain CPV-YNR which is attenuated by canine parvovirus virulent strain CPV-YN. The invention relates to a medicine for diseases caused by parvovirus, belonging to the field of prevention and control of canine parvovirus. Background technique [0002] Canine parvovirus was first discovered in 1978 (AppelM J, Scott FW, CarmichaelL E. Isolation and immunization studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet Rec, 1979, 105(8): 156-159.) Since then, the disease has been popular all over the world, and my country confirmed the existence of canine parvovirus infection in 1982 (Liang Shizhe, Qu Chuanmei, Wei Xiren, etc. Research on canine infectious enteritis I-parvovirus particles detected in dog feces with diarrhea. Shanghai Animal Husbandry and Veterinary Bulletin, 1982, 2 (4): 172. In the following year, Xu Hankun...

Claims

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Application Information

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IPC IPC(8): C12N7/08A61K39/23A61P31/20A61P1/00A61P1/04A61P9/00C12R1/93
Inventor 刘大飞张洪英曲联东王牟平刘立奎
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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