Tissue culturing method for Chinese yam

Abstract

The tissue culture method of Chinese yam includes obtaining bud block as explant, sterilizing bud block, inoculating, provisional planting in greenhouse and other steps. The present invention has the innovations of using bud block as explant to result in less virus, high inoculation efficiency, stable heritability, etc.; improved tissue culture program including inducing rooting, cutting the base part of the bud seedling, soaking in NAA solution of 50 ml / L concentration, inserting in disinfected fine sand for provisional planting and applying diluted MS solution and nutrients and resulting in decreased comprehensive production cost of tissue culture. The present invention makes it possible to realize large scaled production of excellent tissue culture seedling of Chinese yam.

Description

Technical field: [0001] The invention relates to a method for tissue culture of yam (also called yam, yam, the same below) seedlings. Background technique: [0002] Yam is a kind of Dioscorea plant that has both food and medicine, and a wide range of industrial uses. It is highly valued in traditional Chinese medicine and is also favored in modern nutritional concepts. The growth cycle of yam is short, generally 150-180 days; the planting area is wide, and it is cultivated in all provinces except Tibet and the Northwest Loess Plateau, especially in Henan, Shanxi, Jiangxi, Guangxi, Jiangsu, Shandong, and Hunan. The global annual demand exceeds 30 million tons, and the price has been on the rise in recent years. [0003] Chinese yam (yam) is traditionally propagated by means of terminal buds, potato cubes, and zero seeds. These methods are costly and are easily attacked by viruses, resulting in degradation of species, reduced yield, and reduced quality. [0004] Propagation ...

AI Technical Summary

Problems solved by technology

No matter the leaves, stems, tubers or spare seeds all grow in the wild field, all kinds of miscellaneous bacteria grow on the surface, and some fungi even grow into the inside of the tissue, making it difficult for surface disinfectants to kill the fungi inside the tissue (disinfectant Too strong toxicity also affects its structural organization) and the material is prone to contamination after inoculation, thus affecting the effectiveness of tissue culture

There are also two obvious defects in the stem segments grown from zero-surplus seedlings: (1) it is difficult to break the dormancy of zero-surplus seeds, and the time cost is large, so it cannot be mass-produced

(2) The explant (shoot tip) is small and the inoculation efficiency is low

Therefore adopting above-mentioned explant can not realize large-scale factory production

[0009] 2. The cultivation process is complicated, the cost is high, and it is difficult to realize large-scale factory production

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