Tissue culturing method for Chinese yam

A technology of tissue culture and yam, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that large-scale industrial production cannot be realized, difficult large-scale industrial production, and surface disinfectants are difficult to kill , to achieve the effects of reducing comprehensive production costs, easy disinfection, and improving vaccination efficiency

Inactive Publication Date: 2005-08-17
李锐忠
View PDF0 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No matter the leaves, stems, tubers or spare seeds all grow in the wild field, all kinds of miscellaneous bacteria grow on the surface, and some fungi even grow into the inside of the tissue, making it difficult for surface disinfectants to kill the fungi inside the tissue (disinfectant Too strong toxicity also affects its structural organization) and the material is prone to contamination after inoculation, thus affecting the effectiveness of tissue culture
There are also two ob

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culturing method for Chinese yam

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] A method for tissue culture of Chinese yam seedlings, comprising the following steps:

[0032] (1) explant -- the acquisition of block bud: obtaining block bud comprises the following steps:

[0033] (A) Select robust tubers with no surface damage by diseases and insect pests, disinfect with 200 times of bromogeramine or 0.1% potassium permanganate, spray once every 10 minutes, spray 5 times, then rinse with tap water, dry, and then cut into each Put a 10-12cm piece in a ventilated place to let the wound dry;

[0034] (B) After 6 hours, put it in sterilized wet sand for germination acceleration, keep the temperature between 20-30°C, and get tuber buds within 20 days. Hundreds of buds can be obtained;

[0035] (2) Disinfection of bulk buds: After the bulk buds grow out, when the diameter is about 0.6cm, cut them off with a sterilized blade, and cut off the root hairs. In the inoculation room, put the cleaned block buds on the ultra-clean workbench, disinfect them with...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Heightaaaaaaaaaa
Login to view more

Abstract

The tissue culture method of Chinese yam includes obtaining bud block as explant, sterilizing bud block, inoculating, provisional planting in greenhouse and other steps. The present invention has the innovations of using bud block as explant to result in less virus, high inoculation efficiency, stable heritability, etc.; improved tissue culture program including inducing rooting, cutting the base part of the bud seedling, soaking in NAA solution of 50 ml/L concentration, inserting in disinfected fine sand for provisional planting and applying diluted MS solution and nutrients and resulting in decreased comprehensive production cost of tissue culture. The present invention makes it possible to realize large scaled production of excellent tissue culture seedling of Chinese yam.

Description

Technical field: [0001] The invention relates to a method for tissue culture of yam (also called yam, yam, the same below) seedlings. Background technique: [0002] Yam is a kind of Dioscorea plant that has both food and medicine, and a wide range of industrial uses. It is highly valued in traditional Chinese medicine and is also favored in modern nutritional concepts. The growth cycle of yam is short, generally 150-180 days; the planting area is wide, and it is cultivated in all provinces except Tibet and the Northwest Loess Plateau, especially in Henan, Shanxi, Jiangxi, Guangxi, Jiangsu, Shandong, and Hunan. The global annual demand exceeds 30 million tons, and the price has been on the rise in recent years. [0003] Chinese yam (yam) is traditionally propagated by means of terminal buds, potato cubes, and zero seeds. These methods are costly and are easily attacked by viruses, resulting in degradation of species, reduced yield, and reduced quality. [0004] Propagation ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 李锐忠
Owner 李锐忠
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap