Docosahexaenoic acid-producing strain and mutation and screening method and application thereof

A docosahexaenoic acid and production strain technology, which is applied in the biological field, can solve problems such as difficulty in resisting competitive pressure, and achieve the effects of saving screening costs, clear purpose, and improving work efficiency

Active Publication Date: 2009-12-02
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN1986822 is a patent just published in 2007, disclosing a cultivation method of Cryptidinosa fermented to produce DHA, the biomass is 20-40g / L, and the oil content of seaweed cells is 20-50%, but there is still a large gap compared with foreign fermentation levels , the main reason is related to the selected strains and fermentation process, it is difficult to resist the competitive pressure brought by foreign advanced technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: The cultivation of seed.

[0049] Inoculate Cryptidinium dinoflagellate (ATCC 30772) with docosahexaenoic acid production ability into the seed medium at an inoculation amount of 1% (v / v), and cultivate it for three generations at 25°C, and take the third generation after culturing for 40 hours. Substitute liquid.

Embodiment 2

[0050] Embodiment 2: the pretreatment of seed solution.

[0051] Dilute 1 mL of the bacterial solution obtained in Example 1 with 9 mL of sterile artificial seawater with a concentration of 30 g / L, place it in a sterilized Erlenmeyer flask covered with glass beads, shake it at room temperature for 5 min, and make the algae Somatic cells were dispersed, filtered through sterilized 6-layer gauze, serially diluted, and counted until the cell concentration was 1.5×10 4 individual / mL.

Embodiment 3

[0052] Embodiment 3: UV mutagenesis

[0053] Spread the bacterial solution obtained in Example 2 on a sterile plate to ensure that the thickness of the bacterial solution is about 2 to 3 mm, and carry out mutagenesis at a distance of 30 cm from a 30W ultraviolet lamp for 1, 3, 5, 9, and 15 minutes, and obtain 5 strains to be screened strain.

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PUM

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Abstract

The invention discloses a docosahexaenoic acid-producing bacterium and a mutation and screening method and application thereof. The strain is classified and named as Crypthecodinium cohnii HX-308, is preserved in China Center for Type Culture Collection, and has the preservation number M208246. The strain is a DHA-producing bacterium with high yield, has the DHA content 1.53 times of a starting strain, stable physiological-biochemical characteristic and stable heritability, and simultaneously can produce polyunsaturated fatty acid through liquid culture and fermentation; the content of unsaturated fatty acid reaches 20.4g / L, wherein the DHA content reaches 8.16g / L; and the strain is a good strain for producing natural DHA, and has excellent industrial production prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-yield unsaturated fatty acid docosahexaenoic acid strain, a mutagenesis screening method for obtaining the strain, and an application of the strain. Background technique [0002] DHA (Docosahexaenoic acid, 22:6Δ4.7.10.13.16.19, full name docosahexaenoic acid) is an important long-chain polyunsaturated fatty acid (PUFA for short), belonging to the omega-3 series . As an essential component of cell membrane synthesis, DHA has the functions of enhancing eyesight and promoting the development of brain cells. It is an essential component for the growth and development of infant brains and the maintenance of normal brain functions for adults. It is known as "brain gold". DHA can also lower blood fat, prevent and treat arteriosclerosis, anti-inflammatory, anti-cancer and other effects. Therefore, DHA has received extensive attention from the food and medical circles. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N15/01C12Q1/04C12P7/64C12R1/89
Inventor 黄和练敏肖爱华任路静金立晶朱婧瑶
Owner NANJING TECH UNIV
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