Molecular marker method and application of rice chalkiness main effect qtl loci

A molecular marker, chalkiness technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of speeding up the selection progress

Inactive Publication Date: 2019-11-08
HUNAN AGRICULTURAL UNIV
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  • Description
  • Claims
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Problems solved by technology

Although the production of hybrid rice in China has reached a relatively high level, there are very few varieties with particularly good rice quality. Therefore, how to quickly and accurately select high-grade and high-quality rice varieties with low chalkiness is the main goal pursued by breeders in the new era

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  • Molecular marker method and application of rice chalkiness main effect qtl loci
  • Molecular marker method and application of rice chalkiness main effect qtl loci
  • Molecular marker method and application of rice chalkiness main effect qtl loci

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Embodiment 1

[0018] Test materials: The high-quality low-chalkiness Java rice variety Lemont was used as the female parent, and the high-chalkiness indica rice variety Miryang 46 was used as the male parent, and the Lemont×4628 Miryang 46 RILs containing 162 lines were constructed by the one-grain method f 6 group.

[0019] Phenotyping

[0020] In 2015, Lemont, Miryang 46 and Lemont / Miryang 46 RILs F were planted in the experimental base of Hunan Agricultural University 6 A group of 162 strains was sown in two phases on May 5 and May 15, with an interval of 10 days between the two phases. They were transplanted on May 30 and June 4 respectively. Single planting, each material was planted with 30 Each plant was planted in 3 rows, with 10 plants in each row, and the row spacing between plants and rows was 20cm×20cm. The date of first earing was recorded respectively, and 148 recombinant inbred lines and their parents Lemont and Miryang 46 with the same earing date were selected for rice ch...

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Abstract

The invention discloses a molecular marking method of a chalkiness-ratio major-effect QTL (Quantitative Trait Locus) in rice of paddy rice. The method comprises the following steps of carrying out PCR (Polymerase Chain Reaction) amplification on the DNA (Deoxyribonucleic Acid) of a to-be-authenticated paddy rice material by utilizing one pair or two pairs of a primer pair of a molecular marker RM20660 or a primer pair of a molecular marker RM7412, carrying out electrophoretic detection on an amplified product, and if a DNA fragment in a corresponding size is amplified, indicating that a low-chalkiness-ratio major-effect QTL qCD6 exists. According to the method, through applying two sieved SSR (Simple Sequence Repeat) markers which are closely interlocked with the chalkiness-ratio major-effect QTL qCD6, which is located on a sixth chromosome of a low-chalkiness-ratio paddy rice variety Lemnot, of the rice, the chalkiness ratio of the paddy rice material is predicted; the selective breeding of a high-quality low-chalkiness-ratio paddy rice new variety is facilitated; the selection progress of a high-quality low-chalkiness-ratio paddy rice variety is quickened.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker method for a main QTL locus of chalkiness, an important quality trait of rice, and also relates to the application of the molecular marker method in breeding low-chalky high-quality rice varieties. Background technique [0002] Under the pressure of huge population and arable land, my country has been focusing on the improvement of rice yield traits, but lags behind in the research and application of high-quality rice. With the improvement of people's living standards, higher requirements are put forward for the quality of rice; in addition, my country's agricultural production methods are undergoing major changes, and large-scale planting is an inevitable trend in the future development of rice production. It is a commodity grain, emphasizing high benefits. Since the price of high-quality rice is about 20-30% higher than that of ordinary rice, without s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 雷东阳旷浩源
Owner HUNAN AGRICULTURAL UNIV
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