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Molecular marker tightly linked with rice large-grain gene GS12 and application

A molecular marker, GS12 technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of limited space for successful application, and achieve the effect of facilitating timely hybrid selection and speeding up the breeding process

Inactive Publication Date: 2017-05-10
广西壮族自治区农业科学院水稻研究所
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the above cloned genes affecting seed size (such as GS3 and qSW5 / GW5) are ubiquitous genetic variations, most of which come from the core materials of current breeding, and there is very limited room for successful application in breeding practice

Method used

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  • Molecular marker tightly linked with rice large-grain gene GS12 and application
  • Molecular marker tightly linked with rice large-grain gene GS12 and application
  • Molecular marker tightly linked with rice large-grain gene GS12 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Acquisition and application of a new gene GS12 tightly linked marker

[0019] (1) Test materials and phenotypic identification

[0020] The large-grain variety K1561 derived from the introgression line of O. sativa was crossed with the high-quality small-grain restorer line G1025 in Guangxi ( figure 1 ), harvested the F 2 generational segregation groups. Then G1025 was further used as the recurrent parent, F 2 The line heterozygous for the target segment in the population was used as the donor parent, and with the help of molecular marker-assisted selection, three consecutive backcrosses and one self-crossing were carried out in Nanning from the spring of 2013 to the autumn of 2014, and the BC isolated in this segment was obtained. 3 f 1 Near isogenic line, and self-crossed to get BC 3 f 2 And two near-isogenic homozygous genotypes: NIL (K1561) (large grain) and NIL (G1025) (small grain).

[0021] The measurement mainly uses the common vernier caliper w...

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Abstract

The invention discloses a molecular mark tightly linked with a rice large-grain gene GS12 and application, and belongs to the field of rice high-yield and high-quality breeding and crop molecular genetics. An F2 separation population and a near-isogenic line thereof are obtained by utilizing hybridization of a large-grain variety K1562 and a small-grain variety G1025 through genetic linkage analysis, a large-grain new gene GS12 on a chromosome 12 of rice is finely located, the large-grain new gene GS12 is located between molecular marks G103 and G108, and the selection efficiency of three marks of G96, G87 and G111 in the region are all about 98 percent; the molecular mark tightly linked with the GS12 is utilized for detecting whether the large-grain variety K1561 and derived varieties (lines) thereof contain the GS12 or not. The molecular mark disclosed by the invention is applied to assisted-selection breeding and pyramiding breeding of rice yield and appearance quality, genotype selection can be carried out on low-generation breeding materials in a seedling stage, the breeding efficiency is increased, and the breeding progress is quickened.

Description

technical field [0001] The invention belongs to the fields of rice high-yield and high-quality breeding and crop molecular genetics, and specifically relates to a closely linked molecular marker of rice large-grain gene GS12 and its application. Background technique [0002] Rice seed size is not only an important yield trait, but also an important quality trait. Studying the molecular regulation mechanism of its natural variation can provide a deeper understanding of the molecular basis of yield and quality, and provide new ideas and genetic resources for the cultivation of new high-yield and high-quality rice varieties. Molecular genetic studies have shown that each sub-trait of rice seed size is a complex quantitative trait controlled by multiple quantitative trait loci. So far, a large number of genes or quantitative trait loci (quantitative trait loci, QTL) that control seed size have been located on the whole genome of rice, but only 16 genes that have been isolated a...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/68
CPCC07K14/415C12Q1/6895C12Q2600/156
Inventor 郭嗣斌李孝琼韦宇刘开强陈颖高国庆
Owner 广西壮族自治区农业科学院水稻研究所
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