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Molecular markers of pear peel all-red bud mutation trait locus, primers and application thereof

A technology of molecular markers and fruit peels, applied in the field of molecular biology, can solve the problems of low breeding efficiency, long breeding cycle, time-consuming and labor-consuming, etc., and achieve the effects of saving maintenance and management, good social and economic benefits, and improving selection efficiency

Inactive Publication Date: 2017-07-07
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle of this method is long, occupies a large area, consumes time and energy, and the breeding efficiency is low

Method used

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  • Molecular markers of pear peel all-red bud mutation trait locus, primers and application thereof
  • Molecular markers of pear peel all-red bud mutation trait locus, primers and application thereof
  • Molecular markers of pear peel all-red bud mutation trait locus, primers and application thereof

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Experimental program
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Effect test

Embodiment 1

[0048] Source of materials: The test materials are the ‘T109’×’Hongzao Su’ hybrid combination colony planted in the hybrid nursery of Zhengzhou Institute of Fruit Science, Chinese Academy of Agricultural Sciences, with 144 individual plants and 2 parental materials.

[0049] The screening method of the InDel molecular marker In1579-1 and InDel molecular marker In1400-1 at the red budding trait site Red of pear peel includes the following steps:

[0050] (1) Use conventional CTAB method to extract 144 Fs of hybrid combination population 1 DNA of young leaves of a single plant and two parental materials;

[0051] (2) Use InDel labeled primer pair to extract the hybrid combination population F described in step (1) 1 The DNA of 8 red-skin single plants and 8 green-skin single young leaves of a single plant are subjected to PCR amplification reaction to obtain amplified products; this PCR amplification reaction uses a 20μL reaction system, the composition and reaction of the reaction syst...

Embodiment 2

[0060] Material source: The test material is the hybrid combination ‘T109’ × ‘Hongzao Su’ planted in the hybrid nursery of Zhengzhou Institute of Fruit Science, Chinese Academy of Agricultural Sciences to obtain F 1 There are 144 individual plants in the individual plant population.

[0061] The application of InDel molecular marker In1579-1 of the red-type bud transformation trait site Red in pear peel in assisted selection breeding of red pear includes the following steps:

[0062] (1) Use conventional CTAB method to extract hybrid combination population F 1 DNA of individual young leaves;

[0063] (2) Use the primer pair labeled with In1579-1 molecule as the primer to amplify the DNA of the hybrid single strain obtained in step (1) to obtain the amplified product. The PCR reaction system and reaction procedure are as follows:

[0064] The PCR amplification system is a 20μL reaction system: Ex Taq 0.1μL; 10×Buffer 2μL; dNTPs (2.5mM each) 1.6μL; Forward Primer (10μM) 0.6μL; Reward Pri...

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Abstract

The invention discloses InDel molecular markers of a pear peel all-red bud mutation trait locus Red and application thereof. Genetic linkage analysis is carried out on a hybrid F1 population genotype of a non-all red bud mutation material 'T109' and a 'red Zaosu' pear all-red bud mutation variety 'red Zaosu' and the trait phenotype of each single plant to acquire molecular markers linked to the bud mutation trait locus. The pear peel all-red bud mutation trait locus Red is located on a chromosome 4 of pear. The InDel molecular markers linked to the pear peel all-red bud mutation trait locus Red are In1579-1 and In1400-1 respectively, and the genetic distances to the pear peel all-red bud mutation trait locus Red are 1.4cM and 2.1cM respectively. The markers are utilized to detect the filial generations of pear peel all-red bud mutation varieties, the peel color can be predicted in advance, the selection efficiency of red-peel pear breeding is greatly improved, and the cost is saved, therefore the molecular markers have good social and economic benefits.

Description

Technical field [0001] The invention belongs to the field of molecular biology, relates to the technical field of pear genetic breeding, and in particular relates to a molecular marker for the full red bud transformation trait site of pear pericarp and its primers and applications. [0002] technical background [0003] Pear is an international bulk fruit and one of the main fruits in our country. Its fruit is rich in nutrients, delicious and juicy, sweet and delicious, and is deeply loved by the people. Peel color is one of the fruit traits that attract people's attention most, and it is an important part of fruit quality and commerciality. In recent years, the concept of healthy consumption has become more and more popular. Red pears are considered to have higher health value and are favored by the market. The selection and breeding of red pear varieties has also received increasing attention from pear breeding research institutions in various countries. In China, the planting a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 薛华柏李秀根杨健王龙王苏珂苏艳丽张慧蓉曹玉芬
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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