Molecular marker of rice major gene bph22 (t) resistant to brown planthoppers and application thereof

A technology for resisting brown planthopper and main gene, which is applied in the field of molecular genetics, can solve problems such as the complexity and instability of insect resistance identification, difficulty in effectively introducing and aggregating insect resistance genes, etc., and achieves convenient identification, convenient and fast detection, and cost saving effect

Active Publication Date: 2012-11-07
GUANGXI UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is an urgent need for insect-resistant varieties carrying new and multiple resistance genes in rice production. Due to the complexity and instability

Method used

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  • Molecular marker of rice major gene bph22 (t) resistant to brown planthoppers and application thereof
  • Molecular marker of rice major gene bph22 (t) resistant to brown planthoppers and application thereof
  • Molecular marker of rice major gene bph22 (t) resistant to brown planthoppers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) White hair / W2183BC 1 f 2 Population construction and phenotyping

[0044] (1) Li et al. (2006) identified resistance to BPH from the transgenic offspring of common wild rice in Guangxi and screened out BPH biotypes 1 and 2, Bangladesh, Mekong River (Vietnam), Jiulongjiang (Vietnam), The six biotypes in Pantnagar (India) all had broad-spectrum high-resistance W2183. BC constructed by crossing, backcrossing and selfing this resistance source with susceptible variety TN1 2 f 2 The population was mapped to the resistance gene, which was initially located between the molecular markers RM6506 and RM273. Reconstruction of BC using the sensual variety Baimao 1 f 2 Fine mapping of resistance genes in populations. Therefore, the present invention screens and develops SSR molecular markers based on PCR technology. In order to find simple and effective molecular markers that are closely linked to bph22(t), the present invention uses the susceptible variety Baimao as the...

Embodiment 2

[0067] Example 2 Verification of Molecular Markers

[0068] 1. Materials and methods

[0069] 1.1 Negative varieties: 20 copies, 14 copies of non-insect-resistant materials from the susceptible varieties Baimao, Bai R54, Teqing (materials preserved by our laboratory), 9311, Nipponbare, TN1, and Baimao×W2183 hybrids.

[0070] Positive varieties: resistant line W2183, Baimao × W2183, Bai R54 × W2183 hybrid offspring 19 insect-resistant materials.

[0071] Molecular marker primers: RM16846, RM16852, RM16853, RM16858, RM16874 and RM16888, the nucleotide sequences of which are respectively shown in SEQ ID No.1-12.

[0072] 1.2 Method

[0073] Genomic DNA of rice samples was extracted by CTAB extraction method (the method is the same as in Example 1). Sample DNA was amplified with primers RM16846, RM16852, RM16853, RM16858, RM16874 and RM16888, respectively. 10 μl system. The 10μl reaction system includes: 10×PCR buffer, 1.0μl; 10mMdNTPs, 0.1μl; 10μM primer, 0.4μl; 5U / μl Taq DN...

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Abstract

The invention provides a molecular marker of rice major gene bph22 (t) resistant to brown planthoppers and an application of the molecular marker of the rice major gene bph22 (t) resistant to the brown planthopper. The inspect-resistant strain W2183 of the rice and the susceptible variety white hair (white R54) (female) of the rice are hybridized and backcrossed to obtain different progenies; thelines of the different progenies are respectively subjected to identification of the character of the resistance to brown planthoppers and genetic linkage analysis to obtain the resistance gene bph22(t) carried by the inspect-resistant strain W2183 and locate the resistance gene between the molecular marker RM16846 and the molecular marker RM16888; and, the molecular marker tightly linked to the resistance gene also comprises one of RM16852, RM16853, RM16858 and RM16874. The molecular maker provided by the invention effectively detects whether the inspect-resistant strain W2183 and the derived varieties (strains) of the inspect-resistant strain W2183 contain the locus of the major gene; the selection efficiency of the rice resistant to the brown planthoppers is greatly enhanced; and, the gene bph22(t)-containing varieties of the rice resistant to the brown planthoppers are obtained.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a molecular marker of rice rice resistant to brown planthopper main gene bph22(t), and also relates to the application of the molecular marker in breeding rice varieties resistant to brown planthopper. Background technique [0002] Rice is one of the main food crops in my country, and its production is directly related to my country's food security, farmers' income increase and rural stability. Rice cultivation is faced with a wide variety of pests and diseases, which pose a serious threat to its yield in severe cases. Among them, the brown planthopper is an explosive and harmful rice pest. Before the 1960s, brown planthoppers only occurred occasionally in some rice fields in my country. Since then, with the changes in climate, environment, planting structure, farming system and cultivation methods, etc., the area of ​​brown planthopper damage has expanded from south to north, t...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 李容柏陈保善黄大辉邱永福黄凤宽张月雄韦燕萍刘芳
Owner GUANGXI UNIV
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