Molecular marker of rice brown-plant-hopper-resistant gene Bph31 (t) and application of molecular marker
A technology of resistance to brown planthopper and molecular markers, applied in the field of molecular biology, can solve the problems of easy loss of resistance, achieve the effect of improving resistance level, speeding up the breeding process, and facilitating timely hybridization and breeding
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Embodiment 1
[0046] Example 1: Acquisition of Molecular Markers
[0047] (1) Gui 1025 / K41 F 2 Construction and phenotyping of family groups and RILs
[0048] 1. In the previous research, a set of O. sativa introductory lines was constructed by using hybridization and backcrossing of O. sativa and cultivated rice IR24, combined with embryo rescue and molecular marker-assisted selection techniques. Through the identification of resistance to brown planthopper, a highly resistant Rice line K41 with brown planthopper and stable agronomic traits. In order to excavate the resistance gene and its closely linked molecular markers in K41, the present invention uses the insect-susceptible variety Gui 1025 as the female parent and the insect-resistant line K41 as the male parent to cross, and the obtained F 1 and then self-intersection, thereby constructing the F 2 separate groups, each F 2 A single plant obtains the corresponding F by selfing 2:3 Family Lineage, 300 F 2 A single plant adopts t...
Embodiment 2
[0070] Example 2: Verification of Molecular Markers
[0071] (1) Materials and methods
[0072] Negative varieties: 30 copies, the susceptible lines Gui 1025, Lemont, and TN1 are all conventional rice materials preserved in our laboratory, and 27 susceptible lines were among the offspring of the Gui 1025 / K41 hybrid combination.
[0073] Positive varieties: 30 copies, 29 copies of the insect-resistant lines in the offspring of the hybrid combination of insect-resistant lines K41 and Gui 1025 / K41.
[0074] Molecular marker primers: MG81, MG96 and RM471.
[0075] The DNA extraction method and the molecular marker analysis method are the same as in Example 1.
[0076] (2) Results
[0077] Using the above method, PCR amplification was performed on the genomic DNA of 60 different samples of rice lines K41, Gui 1025, Lemont, TN1, etc. respectively. The results showed that the corresponding 140bp fragments, 184bp fragments and 198bp fragments could be amplified in the positive sam...
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