Molecular marker of rice brown-plant-hopper-resistant gene Bph31 (t) and application of molecular marker

A technology of resistance to brown planthopper and molecular markers, applied in the field of molecular biology, can solve the problems of easy loss of resistance, achieve the effect of improving resistance level, speeding up the breeding process, and facilitating timely hybridization and breeding

Inactive Publication Date: 2017-02-22
南宁维尔凯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, recent studies have found that rice varieties with only one major insect-resistant gene are prone to loss of resistance in production, while some varieties with minor genes play a role in long-lasting resistance through modification of the main gene. important role

Method used

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  • Molecular marker of rice brown-plant-hopper-resistant gene Bph31 (t) and application of molecular marker
  • Molecular marker of rice brown-plant-hopper-resistant gene Bph31 (t) and application of molecular marker
  • Molecular marker of rice brown-plant-hopper-resistant gene Bph31 (t) and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Acquisition of Molecular Markers

[0047] (1) Gui 1025 / K41 F 2 Construction and phenotyping of family groups and RILs

[0048] 1. In the previous research, a set of O. sativa introductory lines was constructed by using hybridization and backcrossing of O. sativa and cultivated rice IR24, combined with embryo rescue and molecular marker-assisted selection techniques. Through the identification of resistance to brown planthopper, a highly resistant Rice line K41 with brown planthopper and stable agronomic traits. In order to excavate the resistance gene and its closely linked molecular markers in K41, the present invention uses the insect-susceptible variety Gui 1025 as the female parent and the insect-resistant line K41 as the male parent to cross, and the obtained F 1 and then self-intersection, thereby constructing the F 2 separate groups, each F 2 A single plant obtains the corresponding F by selfing 2:3 Family Lineage, 300 F 2 A single plant adopts t...

Embodiment 2

[0070] Example 2: Verification of Molecular Markers

[0071] (1) Materials and methods

[0072] Negative varieties: 30 copies, the susceptible lines Gui 1025, Lemont, and TN1 are all conventional rice materials preserved in our laboratory, and 27 susceptible lines were among the offspring of the Gui 1025 / K41 hybrid combination.

[0073] Positive varieties: 30 copies, 29 copies of the insect-resistant lines in the offspring of the hybrid combination of insect-resistant lines K41 and Gui 1025 / K41.

[0074] Molecular marker primers: MG81, MG96 and RM471.

[0075] The DNA extraction method and the molecular marker analysis method are the same as in Example 1.

[0076] (2) Results

[0077] Using the above method, PCR amplification was performed on the genomic DNA of 60 different samples of rice lines K41, Gui 1025, Lemont, TN1, etc. respectively. The results showed that the corresponding 140bp fragments, 184bp fragments and 198bp fragments could be amplified in the positive sam...

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Abstract

The invention discloses a molecular marker of a rice brown-plant-hopper-resistant gene Bph31 (t) and application of the molecular marker. Varieties K41 with high brown-plant-hopper resistance and varieties Gui1025 with high brown-plant-hopper sensitivity are hybridized to obtain F2:3 family population and recombinant selfing line population by the aid of heredity linkage analysis, the rice brown-plant-hopper-resistant gene Bph31 (t) on a fourth chromosome of rice is precisely positioned and is positioned in an interval between molecular markers MG81 and RM471, and the individual resistant plant selection efficiency of a single molecular marker MG96 in the interval is 97% approximately. The molecular marker which is in linkage with the gene further comprises the molecular markers MG81 and RM471. The molecular marker and the application have the advantages that the molecular marker can be used for detecting the insect-resistant varieties K41 and derived varieties (lines) of the insect-resistant varieties K41 to determine whether the insect-resistant varieties K41 and the derived varieties (lines) contain the gene or not, and accordingly brown-plant-hopper-resistant rice varieties with the brown-plant-hopper-resistant gene Bph31 (t) can be screened.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of molecular biology, in particular to the molecular marker of the rice brown planthopper resistance gene Bph31(t) and its application. [0003] 【Background technique】 [0004] Brown planthopper is an important pest in rice production. It not only directly harms rice, but also spreads other diseases and viruses during feeding, which seriously threatens the safe production of rice in my country. Discovering and identifying new insect-resistant genes and breeding insect-resistant varieties are the most economical and effective means of control. [0005] Since the 1960s, scientists at home and abroad began to screen and identify brown planthopper-resistant materials, and a large number of insect-resistant resources have been found in cultivated rice and wild rice materials. Up to now, according to literature statistics, a total of 36 brown planthopper resistance genes have been identified. , 21 of which are domi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 郭嗣斌李孝琼韦宇刘开强陈颖高国庆
Owner 南宁维尔凯生物科技有限公司
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