A method for cultivating rice varieties resistant to rice blast

A technology for resistance to rice blast and rice blast, applied in the field of breeding, can solve problems such as the contradiction between strong resistance and high yield, and achieve the effects of improving the level of resistance spectrum and lasting resistance, broadening the genetic distance, and prolonging the service life.

Active Publication Date: 2019-01-11
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to relevant literature research, there is a certain contradiction between strong resistance and high yield.

Method used

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  • A method for cultivating rice varieties resistant to rice blast
  • A method for cultivating rice varieties resistant to rice blast
  • A method for cultivating rice varieties resistant to rice blast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Development of a functional marker of the panicle blast resistance gene Pb1

[0047] 1. Primer design

[0048] Pb1 gene: genebank accession number AB570371.1; P5 gene: genebank accession number AB570370.1.

[0049] Pb1 is located in a tandem repeat of 60 kb, and Pb1 is located in the second repeat, corresponding to P5 in the first repeat. The protein encoded by P5 has only one more glutamic acid than Pb1. Both Pb1 and P5 have panicle blast resistance, but Pb1 has significantly higher panicle blast resistance than P5. The difference in the panicle resistance of the two genes is not due to this glutamic acid. The difference was caused by the gene expression level of Pb1 was significantly higher than that of P5. By comparing the upstream promoter region of the Pb1 and P5 gene coding regions, it was found that the promoter sequence of the upstream 1016bp of the gene coding region was completely matched ( figure 1 ), and the susceptible varieties (Nip is Nipponb...

Embodiment 2

[0076] Example 2 Pita, Piz-t functional markers and primers

[0077]The Pita and Piz-t functional markers and primers all used molecular markers and primer sequences already published in the prior art (Table 1). A 1042bp fragment was detected by using Pita primers, while Npita primers could not amplify the target fragment. Such a material contains the Pita gene; when using Piz-t functional marker primers to amplify two different types of materials, there is a 160bp target band Only A base type (including Piz-t gene), containing a 221bp target band only G base type (excluding Piz-t gene), 331bp target band is A base type (containing Piz-t gene ) and G base type (excluding Piz-t gene) shared bands.

[0078] The experimental procedure for the detection of the disease resistance gene Pita was based on reference [1], with slight modifications: ①In the amplification program, 72°C extension was used for 1 min; ②Electrophoresis was performed using 4% polyacrylamide gel.

[0079] The...

Embodiment 3

[0086] Example 3 Evaluation of resistance gene polygenic aggregation mode "Pita+Pb1+Piz-t".

[0087] Using the molecular markers in Example 1, 227 pre-test materials in Jiangsu Province in 2015 were genotyped for disease resistance genes Pita, Pb1 and Piz-t. The results showed that: among 15 lines (including Pita+Pb1+Piz-t), 7 materials had a comprehensive disease index lower than 4.0 (above medium resistance), and the combination probability of medium resistance to rice blast was 46.47%; 51 lines (excluding Pita+Piz-t) Pb1+Piz-t), only 7 materials had a comprehensive disease index lower than 4.0 (above moderate resistance), and the combined probability of moderate resistance to rice blast was 13.73%; the success probability of moderate resistance to rice blast increased by 32.74 percentage points.

[0088] Table 2 Comparison of the mean value of rice blast correlation index of different taxa in the aggregation pattern "Pita+Pb1+Piz-t"

[0089]

[0090] Remarks: Above medi...

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Abstract

The invention discloses a method for cultivating a rice blast-resisting rice variety. The method comprises the following steps: selecting parents: verifying a rice blast resistance gene of a rice material, and combining and preparing superiority combinations according to the following rules: at least one of the parents contains the relationship of a main variety of which the accumulative promotion area is 5 million mu or more, a rice blast-resisting multi-gene pyramiding mode Pita+Pb1+Piz-t is met, and resistance genes are complementary; moderately broadening the amphilepsis distance according to a parent family tree and form difference, enabling the yield super-parent advantage of F1 to be 8 percent to 15 percent, and enabling the super / mid-parent advantages of the plant height and the sowing period of the F1 to be both smaller than 3 percent; carrying out pyramiding breeding according to a breeding goal, thus obtaining the rice blast-resisting rice variety, wherein the breeding goal at least comprises a goal of pyramiding Pita+Pb1+Piz-t and a goal of resisting neck blast. The yield and the resistance of the rice variety bred by utilizing the method are well coordinated, and a resistant spectrum and the durable resistance level of the neck blast are particularly and obviously increased.

Description

technical field [0001] The invention relates to breeding, in particular to a method for cultivating rice varieties resistant to rice blast. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the population uses rice as a staple food. Rice blast caused by Magnapothe oryzae is one of the most serious diseases to rice production, causing serious grain loss every year. Breeding and promotion of disease-resistant varieties is the most environmentally friendly, economical and effective way to control rice blast. Traditional rice blast resistance breeding relies on the identification of resistance phenotypes, which is easily affected by environmental conditions and human errors. The resistance gene is not clear, and the probability of successful breeding for rice blast resistance is low. With the application of molecular marker technology, polygenic aggregation of disease resistance genes has become more and more a reali...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02C12Q1/6895
CPCA01H1/02C12Q1/6895C12Q2600/13
Inventor 林添资龚红兵景德道孙立亭余波钱华飞曾生元李闯姚维成盛生兰周义文
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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