30results about How to "Increase resistance level" patented technology

The invention discloses a method for breeding high-yield japonica rice species capable of efficiently preventing rice blast. The method comprises the following steps: (1) hybridizing a rice blast preventing parent with a high-yield parent to obtain a hybrid F1 generation, planting the hybrid F1 generation, and harvesting the F2 generation seeds; (2) sowing the F2 generation seeds in the planting area in a proper time, and selecting the single plants to obtain F3 generation plants; (3) mixing the selected F3 generation plants, threshing, sowing in a proper time, planting single plants, in the booting stage, using the rice blast mixed bacterium liquid in the area which is suitable for promotion of the species to carry out inoculation, and choosing the single plants to obtain the F4 generation plants; (4) choosing segregation generation more than F4 generation, choosing the plants, and obtaining the seeds of target species. According to the method, a rice blast inoculation technology and a high-yield species small area selecting technology are organically combined; the rice blast resisting gene and high-yield gene are effectively combined; the yield level and the resistance level are both enhanced, the vitality of the novel species is improved, and the rice blast resistant level of the novel species is effectively enhanced at the same time.

The invention discloses a method for cultivating a rice blast-resisting rice variety. The method comprises the following steps: selecting parents: verifying a rice blast resistance gene of a rice material, and combining and preparing superiority combinations according to the following rules: at least one of the parents contains the relationship of a main variety of which the accumulative promotion area is 5 million mu or more, a rice blast-resisting multi-gene pyramiding mode Pita+Pb1+Piz-t is met, and resistance genes are complementary; moderately broadening the amphilepsis distance according to a parent family tree and form difference, enabling the yield super-parent advantage of F1 to be 8 percent to 15 percent, and enabling the super/mid-parent advantages of the plant height and the sowing period of the F1 to be both smaller than 3 percent; carrying out pyramiding breeding according to a breeding goal, thus obtaining the rice blast-resisting rice variety, wherein the breeding goal at least comprises a goal of pyramiding Pita+Pb1+Piz-t and a goal of resisting neck blast. The yield and the resistance of the rice variety bred by utilizing the method are well coordinated, and a resistant spectrum and the durable resistance level of the neck blast are particularly and obviously increased.

The invention belongs to the technical field of citrus quality improvement, and particularly relates to a plant expression vector for antimicrobial peptide induced expression and application thereof. The invention aims to provide a new option for improving citrus canker resistance. The technical scheme is an expression cassette for antimicrobial peptide induced expression. The invention is characterized in that the citrus canker pathogen is adopted to efficiently induce the expression of the expression promoter control cecropin antimicrobial peptide. The invention also relates to a plant expression vector containing the expression cassette and a host cell containing the plant expression vector. The invention also relates to a method for enhancing citrus canker resistance. The antimicrobial peptide gene mAATCB under the control of the canker high-efficiency promoter is integrated into the citrus genome, thereby implementing quick high-level expression of the antimicrobial peptide gene when the citrus is infected by the canker pathogen, and further enhancing the citrus canker resistance.

The invention discloses a pile foundation construction technology of a bored cast-in-place pile. The pile foundation construction technology of the bored cast-in-place pile includes the following technological processes of measurement and positioning, drilling machine platform erection, protective cylinder burying, drilling machine positioning, slurry preparation, drilling, first hole cleaning, manufacture and installation of reinforcing cages, placement of conduits, second hole cleaning, underwater concrete pouring and size and strain detection. According to the pile foundation construction technology of the bored cast-in-place pile, the construction steps of the bridge pile foundation are completed in detail and comprehensively, the bridge construction is a large-scale project and requires high construction accuracy and strict quality, therefore, the pile foundation construction technology can improve the resistance grade of the bridge pile foundation to natural disasters such as earthquakes and hurricanes, and it is ensured that the bridge engineering achieves a setting service life period.

The invention discloses a rice WD40-rich repeat protein OsWD40-141 and a coding gene and application thereof. The invention firstly discloses application of any one of proteins in regulation of plantbacterial leaf blight resistance; the proteins include A1) a protein with an amino acid sequence of SEQ ID No. 1, A2) a fusion protein obtained by connecting a tag to the N end or/and C end of the amino acid sequence shown in SEQ ID No. 1, and A3) a protein which has 90% or more identity with the protein shown in A1, has the same functionality, and is obtained by subjecting the amino acid sequenceshown in SEQ ID No. 1 to substitution and/or deletion and/or addition of one or several amino acid residues. Further disclosed is a method for growing a mutant plant having enhanced resistance to bacterial leaf blight. The invention provides an efficient breeding means to create a bacterial leaf blight resistant material based on protein OsWD40-141; the efficient breeding means has important application value.

The invention belongs to the technical field of engineering protection and discloses a staggered distribution plate type steel-based composite missile shielding structure. The staggered distribution plate type steel-based composite missile shielding structure is of a plate type structure constituted in the mode that high-hardness core bodies are embedded into thin-wall box grid metal base bodies;a plurality of metal box grids on each layer are continuously arranged and distributed; the metal box grids on the upper layer and the lower layer are arranged and distributed in a staggered mode; thethin-wall metal box grids are overall of a vertical staggered distribution plate type structure formed by integral casting; the upper end faces of the thin-wall metal box grids on the upper layer areopen, and the lower end faces of the thin-wall metal box grids on the lower layer are open; each thin-wall metal box grid is internally provided with the corresponding high-hardness material servingas a missile-shielding structure filling core body; and the high-hardness materials and the staggered distribution metal box grids are bonded into a whole through adhesion agents to form the plate type composite structure. The staggered distribution plate type composite missile-shielding structure has the characteristics of being high in anti-impact force, good in impact toughness, excellent in performance, reliable, practical, convenient and rapid to construct, capable of resisting repeated strike, replaceable, easy to repair and the like.

The invention discloses a prestressed concrete pipe pile joint. The prestressed concrete pipe pile joint comprises an end plate connected with a pipe pile; the end plate comprises a sub end plate anda main end plate; a boss is arranged at the middle part of the sub end plate; a hole groove is formed in the side surface of the boss; a groove matched with the boss in shape is formed in the middle part of the main end plate; a hole groove corresponding to the hole groove in the boss is formed in the side wall of the groove; the sub end plate is connected with the main end plate through a fastening piece; in addition, the main end plate can be further arranged as a through hole matched with the boss; a through hole can also be formed in the boss; and the through hole communicates with the hole groove. The prestressed concrete pipe pile joint has the advantages of simple operation, low cost, firm connection and high safety; and the prestressed concrete pipe pile joint is suitable for connection of prestressed concrete pipe piles.

The invention discloses a method for improving germplasm creation efficiency of radish, which comprises the following steps: S1, preparation of rootstocks and scions: selecting a hybrid variety easy to generate embryos as a rootstock material, selecting a hybrid variety with brittle green skin color and flesh color as a scion material, and sowing and vernalizing rootstock and scion seeds; S2, field planting; S3, grafting environment management: controlling the environment temperature to enable plants to grow, and irrigating roots with a chlormequat chloride diluent; S4, grafting time selection: taking the second and later primary branches of the rootstocks as grafting parts, and selecting primary branches with the length of 6-10 cm as the scions; S5, grafting: grafting the scion buds on rootstock plants; and S6, management after grafting: after grafting is completed, managing grafted plants by controlling the growth environment temperature. The method can effectively improve the embryogenesis rate of free microspores of the material difficult to embryogenesis, accelerate the purification speed of the radish material and improve the germplasm creation efficiency.

The present invention discloses a primer for auxiliary identification of a soybean anti-mosaic virus SC7 and an identification method thereof. A BARCSOYSSR_02_0631 molecular marked special primer is used to conduct early progeny selection identification on related sites of a soybean strain anti-mosaic virus SC7. The method not only avoids spread of mosaic virus in the traditional inoculation identification method, but also reduces the subsequent workload of breeding and breeding cycles, so as to quickly screen a resistant high-yield soybean breeding material. The method has high economic efficiency, can be applied to the resistance breeding of soybean progeny, can improve breeding efficiency and resistance level of soybean mosaic virus, and improve the pertinency of breeding purpose.

The invention relates to a release agent for flue-cured tobacco quinclorac phytotoxicity. The release agent comprises the following components in percentage by weight: 1.5%-37.5% of diethyl aminoethyl hexanoate and 62.5%-98.5% of L-cysteine. According to the release agent for flue-cured tobacco quinclorac phytotoxicity disclosed by the invention, the combined action of the function of diethyl aminoethyl hexanoate for adjusting plant endogenous hormone balance and the detoxifying effect of the L-cysteine are comprehensively utilized for effectively relieving quinclorac phytotoxicity symptoms, so that quinclorac teratogenetic tobaccos can remarkably recover normal botanical characters and inherent chemical quality; moreover, the material price is low, the compatibility is strong, the preparation is simple, the effect is obvious, the safety is high, the pertinence is strong and the practical value is high.