The invention discloses the application of rice E3
ubiquitin ligase BISG1 and its encoding
gene in rice
disease resistance breeding, belongs to the field of
gene engineering technology, and discloses the application of rice E3
ubiquitin ligase BISG1 in regulating rice blast resistance, improving rice blast resistance or cultivating transgenic rice resistant to rice blast. The invention constructs overexpression and
gene knockout vectors of rice BISG1 gene, transfers the vectors into Taipei 309
receptor material, obtains BISG1 overexpression plants and knockout plants, and analyzes the
disease resistance of the obtained transgenic plants. The results show that knocking out BISG1 gene significantly improves the resistance level of rice to rice blast, indicating that BISG1 negatively regulates the
disease resistance of plants, and its encoding gene BISG1 can be used as a
target gene to improve
plant disease resistance, has good application prospects in cultivating disease-resistant rice varieties, and lays an important foundation for rice resistance breeding.