q srbsdv6 (southern rice black-streaked dwarf virus 6) and molecular marker method thereof

A black-streaked dwarf disease and southern rice technology, applied in the field of molecular genetics, can solve the problems of resistance gene mining and disease-resistant breeding research that have not yet been reported, and achieve convenient and fast detection, not easy to be affected by the environment, and convenient and fast identification Effect

Active Publication Date: 2015-03-25
广西壮族自治区农业科学院水稻研究所
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Problems solved by technology

However, there are very few studies on the molecular mechanism of the southern rice black-streaked dwarf resistance gene, and the research on the resistance of the host has just started. At present, there are only a few reports on the selection of resistant materials, resistance gene mining and disease-resistant breeding. Research not yet reported

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  • q srbsdv6 (southern rice black-streaked dwarf virus 6) and molecular marker method thereof
  • q srbsdv6 (southern rice black-streaked dwarf virus 6) and molecular marker method thereof
  • q srbsdv6 (southern rice black-streaked dwarf virus 6) and molecular marker method thereof

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Embodiment Construction

[0026] The specific embodiment of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0027] Materials and Methods:

[0028] Step 1: construction of genetic analysis segregation population;

[0029] The wild rice material y11 resistant to southern rice black-streaked dwarf disease was used as the male parent, and the southern rice black-streaked dwarf variety Guanghui 998 was used as the female parent to prepare F 1 Hybrid, and then backcross with Guanghui 998 as the female parent and 998 / y11 hybrid as the male parent until BC 3 f 1 , followed by selfing and multiplication to obtain BC 3 f 7 group. The introduction line Nong 14 with high resistance to southern rice black-streaked dwarf was crossed with the recipient parent and then selfed to obtain the resistance segregation population F2:3 family.

[0030] Step 2: Identification of resistance to black-streaked dwarf of southern rice by indoor inoculation ident...

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Abstract

The invention provides a q SRBSDV6 (Southern rice black-streaked dwarf virus 6) and a molecular marker method thereof. BC3F7 is obtained through hybridization, backcross and selfing of Guangxi common wild rice material y11 and the susceptible variety Guanghui 998 and then is subjected to association analysis and genetic linkage analysis to obtain the q SRBSDV6, and the q SRBSDV6 is positioned between RM20148 and RM 20297; when the molecular marker RM20148 primer has q SRBSDV6, the amplification band is 129 bp band; when the molecular marker RM 20297 primer has q SRBSDV6, the amplification band is 172 bp band. According to the invention, the molecular markers of q SRBSDV6 genes can be adopted to detect whether the resistant material y11 and the derived varieties (series) contain the q SRBSDV6, the resistance level of the SRBSDV6 can be predicted, and the selective efficiency of the q SRBSDV6 rice can be greatly improved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a Guangxi common wild rice material y11 resistant to southern rice black-streaked dwarf locus qSRBSDV6 and a molecular marking method thereof. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as the main food. Southern rice black-streaked dwarfism was first discovered in 2001, and the disease has spread rapidly in just a few years, causing great losses to the vast rice areas in southern my country and northern Vietnam. Different from common rice black-streaked dwarf disease, southern rice black-streaked dwarf disease is a rice virus disease mainly transmitted by white-backed planthopper, and its pathogenic virus is Southern rice black-streaked dwarf virus (Southern rice black- streaked dwarf virus (SRBSDV for short) is a new species of group 2 of the genus Fijivirus of the Reoviridae fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 李丹婷农保选夏秀忠秦碧霞张宗琼杨行海曾宇邓国富李战彪刘开强
Owner 广西壮族自治区农业科学院水稻研究所
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