3 results about "Rice dwarf virus" patented technology

The invention provides an odorant binding protein sourced from cicada and application of the odorant binding protein in prevention and treatment of rice dwarf disease. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP8 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; the RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP8 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs after 2 hours. Therefore, the silent cicada NcinOBP8 can influence the perception of the cicada on the rice volatile matters.

According to the odorant binding protein for the black-tailed leafhopper and the application of the odorant binding protein, by interfering the expression quantity of the binding protein in the black-tailed leafhopper, the perceptual ability of the black-tailed leafhopper to volatile matters induced by rice dwarf viruses can be effectively inhibited, so that the effect of preventing and treating the rice dwarf viruses is achieved. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP7 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; an RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP7 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs any more. Therefore, the silence of the black-tail leafhopper NcinOBP7 can influence the perception of the black-tail leafhopper on the rice volatile matters, and subsequently speculates to inhibit the feeding of the black-tail leafhopper on the virus-infected rice and influence the transmission of viruses.