Application of RING1 protein for improving RDV (rice dwarf virus) resistance of plants

A dwarf disease and protein technology, applied in the application field of RING1 protein in improving plant resistance to rice dwarf virus, can solve problems such as unmentioned interaction
CN103194431AInactive Publication Date: 2013-07-10PEKING UNIV

Patent Information

Authority / Receiving Office
CN Ā· China
Current Assignee / Owner
PEKING UNIV
Publication Date
2013-07-10
Estimated Expiration
Not applicable Ā· inactive patent

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Abstract

The invention discloses application of RING1 protein for improving RDV (rice dwarf virus resistance) of plants, and particularly provides application of protein RING1 or a coding gene of the protein RING1 or a recombinant vector containing the coding gene for regulating RDV resistance of plants. The amino acid sequence of the protein RING1 is the sequence one in the sequence table. The experiment disclosed by the invention proves that the RING1 is E3 protein-ubiquitin ligase and capable of interacting with RDV coat protein P2 to promote the P2 to be degraded by 26S proteosome, and the cumulant of the P2 can be reduced by excessively expressing the RING1 protein in rice, thereby inhibiting the amplification of RDV and improving the anti-virus capability of rice.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to the application of RING1 protein in improving plant resistance to rice dwarf virus. Background technique

[0002] Rice dwarf virus (RDV) is a member of the genus Phytoreovirus in the Reoviridae family. RDV is the pathogen that causes rice dwarf disease, which can infect rice and cause severe rice yield reduction. The spread of RDV requires the aid of the insect vector leafhopper. RDV is a double-stranded RNA virus whose genome consists of twelve double-stranded RNAs. According to the mobility of these twelve double-stranded RNAs in polyacrylamide gel electrophoresis from slow to fast, they are named S1 to S12 respectively. The RDV genome encodes at least seven structural proteins, including P1, P2, P3, P5, P7, P8, and P9; five nonstructural proteins, including Pns4, Pns6, Pns10, Pns11, and Pns12.

[0003] P2 is the coat protein of RDV, encoded by S2, which can interact with rice ...

Claims

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