RT-PCR detection method of south rice black-streaked dwarf virus (SRBSDV)
A black-streaked dwarf virus and RT-PCR technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of time-consuming and consumables, and achieve the effect of saving reagent consumption and time.
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[0045] Use this pair of specific primers to do a RT-PCR on the RBSDV disease strains from Zhejiang and the SRBSDV disease strains from 5 places in Hunan, see figure 2 As a result, only the SRBSDV diseased strains from five places in Hunan were positive, and the expected band of 477 bases was amplified, and the RBSDV diseased strains from Zhejiang and the healthy rice seedling control were negative. It has been illustrated that the pair of specific primers of the present invention are specific to the southern rice black-streaked dwarf virus, and it is enough to accurately detect SRBSDV by doing an RT-PCR to RBSDV and SRBSDV viruses, and can distinguish RBSDV and SRBSDV two kinds of viruses Accurately distinguish without doing nested RT-PCR.
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