Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

RT-PCR detection method of south rice black-streaked dwarf virus (SRBSDV)

A black-streaked dwarf virus and RT-PCR technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of time-consuming and consumables, and achieve the effect of saving reagent consumption and time.

Inactive Publication Date: 2010-05-26
HUNAN AGRICULTURAL UNIV
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Regarding the detection technology of SRBSDV, Zhou Guohui from South China Agricultural University and others designed two pairs of primers based on the nucleotide sequence of the tenth RNA segment of the virus for nested RT-PCR detection. This method requires 2 PCRs, which is time-consuming and consumable.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • RT-PCR detection method of south rice black-streaked dwarf virus (SRBSDV)
  • RT-PCR detection method of south rice black-streaked dwarf virus (SRBSDV)
  • RT-PCR detection method of south rice black-streaked dwarf virus (SRBSDV)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Use this pair of specific primers to do a RT-PCR on the RBSDV disease strains from Zhejiang and the SRBSDV disease strains from 5 places in Hunan, see figure 2 As a result, only the SRBSDV diseased strains from five places in Hunan were positive, and the expected band of 477 bases was amplified, and the RBSDV diseased strains from Zhejiang and the healthy rice seedling control were negative. It has been illustrated that the pair of specific primers of the present invention are specific to the southern rice black-streaked dwarf virus, and it is enough to accurately detect SRBSDV by doing an RT-PCR to RBSDV and SRBSDV viruses, and can distinguish RBSDV and SRBSDV two kinds of viruses Accurately distinguish without doing nested RT-PCR.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an RT-PCR detection method of a south rice black-streaked dwarf virus (SRBSDV). In the detection method, SRBSDV specific primers are used for carrying out RT-PCR amplification on a sample to be detected and then carrying out electrophoretic identification. The specific primers are obtained by the method that by utilizing the conservatism of the evolution of viral nucleotide sequences, the tenth section of RNA of SRBSDV and nucleotide sequences of the tenth section of RNA of similar viruses are searched and downloaded from NCBI / GenBank, the sequences are compared by utilizing MEGA4.0 software, and then a pair of primers which are shared by all the SRBSDV nucleotide sequences reported by different researchers and are different from other viral sequences are found out from a file with an extension name of mas. Proved by a BLAST result, sequences which 100 percent cover the primers and 100 percent same as the primers are all the SRBSDV nucleotide sequences. The primers can be used for accurately separating SRBSDV diseased plants from ordinary RBSDV diseased plants without a nested RT-PCR detection.

Description

Technical field: [0001] The invention relates to a detection method of southern rice virus, in particular to a RT-PCR (reverse transcription polymerase chain reaction) detection method of southern rice black streaked dwarfvirus (SRBSDV). Background technique: [0002] Ordinary rice black-streaked dwarf virus disease is a serious viral disease in Jiangsu and Zhejiang, causing serious losses every year. In 2008, Zhejiang Academy of Agricultural Sciences and South China Agricultural University reported that a new viral disease occurred in Guangdong and Hainan provinces respectively. South China Agricultural University tentatively named the virus as southern rice blackstreaked dwarf virus SRBSDV), and Zhejiang Academy of Agricultural Sciences called it rice black streaked dwarf virus 2 (rice black streaked dwarf virus 2, RBSDV2). In 2009, a dwarf disease similar to these two viral diseases occurred in a large area in Hunan Province. According to preliminary statistics, the affe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 高必达
Owner HUNAN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products