Method for rapidly identifying rice black-streaked dwarf virus and southern rice black-streaked dwarf virus

Abstract

The invention provides a method for rapidly identifying the rice black-streaked dwarf virus and southern rice black-streaked dwarf virus, belonging to the field of plant protection. Three primers are designed according to the nucleotide sequence of the rice black-streaked dwarf virus and southern rice black-streaked dwarf virus, which are respectively [A] RB1_S9_F, [B] RB2_S9_F and [C] RB_S9_R, wherein the combination of A and C detects the rice black-streaked dwarf virus and the combination of B and C detects the southern rice black-streaked dwarf virus. After the total RNA is extracted fromthe samples, the two viruses of the rice black-streaked dwarf virus and the southern rice black-streaked dwarf virus can be detected by only one RT-PCR, thus realizing the identification of two viruses. Compared with the traditional method in which one RT-PCR can only detect one virus, the invention greatly reduces cost, reduces time, and is a detection method which is special, sensitive, economic and convenient.

Description

Technical field [0001] The invention relates to a method for quickly identifying rice black-streaked dwarf virus and southern rice black-streaked dwarf virus, belonging to the field of plant protection. Background technique [0002] Rice black-streaked dwarf virus (Rice black-streaked dwarf virus, RBSDV) is a serious virus disease on rice. It belongs to the Fijivirus of the plant reovirus family (Fijivirus). Laodelphax striatellus spreads. In the early stage, the symptoms of the field are mainly dwarfing and dark green leaves. In the later stage, wax drop-like protrusions appear on the leaves, sheaths and stems, and then black bars are formed, which have a great impact on the yield of rice. Can lead to no yield. In addition to infecting rice, the virus can also infect corn (maize rough dwarf disease), wheat (wheat green dwarf) and a variety of gramineous weeds. It was popular in Jiangsu, Shanghai, and Zhejiang of China in 1963 and 1966, and it broke out in many corn and rice pr...

AI Technical Summary

Problems solved by technology

Since these two viruses have very similar characteristics in many aspects, traditional plant virus detection methods, such as electron microscope observation (similar in size and shape of virus particles), traditional biological inoculation identification (symptoms, mediators, host range similar), serological detection (sequences still have high homology) and other methods are difficult to distinguish

At present, molecular biology detection methods are mostly used in the detection of RBSDV, and RT-PCR is the most commonly used method. It has also been reported that RT-PCR was used to detect RBSDV, but it has not been seen that RBSDV and RBSDV can be detected simultaneously. SRBSDV, and the method of distinguishing, this method only needs one RT-PCR to realize the identification of the two viruses, and on the premise of ensuring the sensitivity and specificity, it simplifies the operation and saves the cost

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