Acquiring method for special small brown rice planthopper for inoculating rice black-streaked dwarf virus

Abstract

The invention discloses a method for obtaining Laodelphax striatellus specially for inoculating black streaked dwarf virus of rice and application thereof in agriculture. The principle of the invention is to obtain Laodelphax striatellus without carrying stripe virus of rice by manual screening, feed the Laodelphax striatellus on rice carrying the black streaked dwarf virus without the stripe virus to obtain virus, transfer the Laodelphax striatellus to healthy rice sprouts without carrying viruses to feed for living through cycle period of the viruses, and further obtain the Laodelphax striatellus carrying the black streaked dwarf virus of the rice without the stripe virus of the rice. The established method for identifying the resistance of a rice variety with the black streaked dwarf virus can suppress interference due to that the Laodelphax striatellus simultaneously transmits the stripe virus of the rice, so that the identifying result is more objective and accurate, and the evaluation of resistance is more scientific and reasonable. The identifying method can be applied in discovery, identification, the evaluation of variety resistance, the breeding of disease-resistant variety, the research on resistance genetic development for rice variety resources and other fields.

Description

technical field [0001] The invention relates to a method for obtaining SBPH specially used for inoculating rice black-streaked dwarf virus and its application in agriculture, belonging to the field of agricultural science and technology. Background technique [0002] Rice black-streaked dwarf virus (Rice black-streaked dwarf virus, RBSDV) is a viral disease transmitted by the striatellus striatellus. It infects rice and causes rice black-streaked dwarf disease. Once the rice is infected, it will not be harvested. Since the disease was discovered in Yuyao County, Zhejiang Province, my country in 1963, it has mainly occurred in indica rice-producing areas in southern Zhejiang in the last century, but it has rarely been found in major japonica rice-producing areas such as Jiangsu. In early 2004, rice stripe blight broke out in Jiangsu and other provinces, and a batch of disease-resistant varieties were rapidly popularized and applied on a large scale, which played a key role in...

AI Technical Summary

Problems solved by technology

[0003] In the face of sudden and explosive viral diseases, it is the most direct and effective method to screen disease-resistant varieties and apply them in production. However, rice black-streaked dwarf and rice stripe blight are both viruses that rely on SBPH for transmission. The characteristics of the disease have added many difficulties to the identification method of resistance to rice black-streaked dwarf disease

First of all, due to the fact that rice stripe leaf blight occurs in most of the current main rice producing areas, SBPH can transmit its pathogen RSV through eggs, which makes SBPH often carry RSV and RBSDV at the same time. The results of identification of resistance to black-streaked dwarf disease are often disturbed by RSV

Secondly, when SBPH is collected from the field for indoor inoculation, the identification results will also be affected in the same way

In addition, there are similar problems in the identification of species resistance by indoor feeding poison inoculation method. Both the SBPH collected in the field and the poison source used for feeding poison are susceptible to RSV interference.

At the same time, SBPH cannot transmit RBSDV through eggs, which makes it impossible for us to obtain SBPH exclusively for inoculation with RBSDV by screening the offspring of infected adults.

The above-mentioned reasons have greatly restricted the breeding of rice black-streaked dwarf resistant varieties, and it is urgent to invent a method for inoculating the rice black-streaked dwarf virus as soon as possible to assist in the establishment of rice black-streaked dwarf virus. The identification method of resistance to black-streaked dwarf disease provides a basic research method for resource excavation and screening, variety selection and genetic law research of rice resistance to black-streaked dwarf disease

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