Hybridoma cell strain excreting monoclonal antibody (MAb) resisting rice blackstreaked dwarf virus (RBSDV) and application of MAb

A technology for dwarfing black stripes and hybridoma cell lines in rice, which can be applied in the fields of antiviral immunoglobulins, analytical materials, biochemical equipment and methods, etc., and can solve problems such as low efficiency

Active Publication Date: 2012-07-04
ZHEJIANG UNIV
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Problems solved by technology

In order to grasp the natural population of SBPH and the disease status of rice, strengthen the early monitoring and early warning of rice black-streaked dwarf disease in my country, and scientifically guide the prevention and control, it is urgent to establish...

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  • Hybridoma cell strain excreting monoclonal antibody (MAb) resisting rice blackstreaked dwarf virus (RBSDV) and application of MAb
  • Hybridoma cell strain excreting monoclonal antibody (MAb) resisting rice blackstreaked dwarf virus (RBSDV) and application of MAb
  • Hybridoma cell strain excreting monoclonal antibody (MAb) resisting rice blackstreaked dwarf virus (RBSDV) and application of MAb

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[0015] Hybridoma cells secreting monoclonal antibody resistant to rice black-streaked dwarf virus were deposited in the Institute of Microbiology, Chinese Academy of Sciences on November 28, 2011, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China Microorganism Culture Collection The General Microbiology Center of the Management Committee, the deposit number is CGMCC No.5537, which can secrete monoclonal antibodies against rice black-streaked dwarf virus.

[0016] Anti-Rice black-streaked dwarf virus monoclonal antibody ascitic fluid indirect ELISA titer up to 10 -6 Above, the antibody type and subclass are IgG1 and kappa chain, and the monoclonal antibody can have a specific immune binding reaction with the 56kD coat protein subunit of rice black-streaked dwarf virus.

[0017] The application of anti-rice black-streaked dwarf virus monoclonal antibody in the virus detection is various immunological detection methods and immunological kits established...

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Abstract

The invention discloses a hybridoma cell strain excreting a monoclonal antibody (MAb) resisting a rice blackstreaked dwarf virus (RBSDV) and the application of the MAb. A coat protein (CP) of the RBSDV is expressed into antigen immune BALB/c mouse by a prokaryotic expression method, and the hybridoma cell strain 5G1 which can stably passage and excrete the MAb resisting the RBSDV is obtained through cell fusion, selection and cloning, wherein the preserving number is CGMCC No. 5537. Ascitic indirect enzyme-linked immuno sorbent assay (ELISA) valence of the 5G1 MAb is over 10<-6>, and the antibody type and the subtype are IgG1 and kappa chain. The antibody excreted by the cell strain and the coat protein of the RBSDV have specific immunobinding reaction. By the dot-ELISA detection method for detecting rice planthopper and the RBSDV in rice and established by using the 5G1 MAb as a core, the virus can be still detected when a single-head small brown rice planthopper is diluted to 1,600 microlitres and sick leaves are diluted (w/v, g/mL) in the proportion of 1:160. The preparation of the MAb resisting the RBSDV and the establishment of the detection method thereof provide technical and material support for the diagnosis, forecast and scientific prevention and control of rice viruses.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting a monoclonal antibody against rice black-streaked dwarf virus and the application of the monoclonal antibody. Background technique [0002] In the mid-1960s, rice black-streaked dwarf disease caused severe damage to cereal crops such as rice, wheat, and corn in many provinces and cities in Zhejiang and East China. The corn planting area was forced to change the cultivation system, and the diseased area decreased rapidly in the next 20 years until it disappeared in the 1970s. In recent years, black-streaked dwarf disease has occurred and prevailed in Zhejiang, Shanghai, Jiangsu, Anhui, Jiangxi and other places due to various factors such as changes in cultivation system, climate warming, and outbreaks of SBPH. [0003] Rice black streaked dwarf disease is caused by rice black streaked dwarf virus (RBSDV), which belongs to the genus Fijivirus of the fam...

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Application Information

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IPC IPC(8): C12N5/20C07K16/10G01N33/577C12R1/91
Inventor 吴建祥周雪平倪跃群刘欢饶黎霞
Owner ZHEJIANG UNIV
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