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Method for obtaining sogatella furcifera carrying SRBSDV (southern rice black-streaked dwarf virus) and application of sogatella furcifera

A technology for black-streaked dwarf virus and white-backed planthopper, which is applied in the biological field, can solve the problems of breeding limitation of resistant varieties, difficult long-term preservation of rice poison sources, etc., and achieves the effect of broadening the time for resistance identification.

Inactive Publication Date: 2012-08-15
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the fact that the disease relies on white-backed planthoppers to spread damage, and the source of rice poisons is extremely difficult to preserve for a long time, the breeding of resistant varieties of southern rice black-streaked dwarf is greatly restricted.

Method used

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  • Method for obtaining sogatella furcifera carrying SRBSDV (southern rice black-streaked dwarf virus) and application of sogatella furcifera
  • Method for obtaining sogatella furcifera carrying SRBSDV (southern rice black-streaked dwarf virus) and application of sogatella furcifera
  • Method for obtaining sogatella furcifera carrying SRBSDV (southern rice black-streaked dwarf virus) and application of sogatella furcifera

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Embodiment Construction

[0024] The present invention utilizes white-backed planthoppers carrying southern rice black-streaked dwarf virus to screen monoclonal antibodies against SRBSDV, and identify the resistance of rice varieties to the virus. The breeding of resistant varieties provides means and material basis, and also provides materials for further research on the interaction between SRBSDV and vector insects.

[0025] Acquisition of white-backed planthopper: collect and capture white-backed planthopper nymphs from rice fields in non-Southern rice black-streaked dwarf virus-occurring areas, and then raise them indoors with non-toxic rice seedlings of non-insect-resistant rice varieties, until white-backed planthoppers become adult After mating, single-headed insects lay eggs alone, randomly select 10% of the white-backed planthoppers in the population, and use RT-PCR and dot-ELISA methods to detect the poisoning status, keep the offspring of non-venomous females and establish an indoor populatio...

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Abstract

The invention discloses a method for obtaining sogatella furcifera carrying SRBSDV (southern rice black-streaked dwarf virus) and an application sogatella furcifera. The method for obtaining the sogatella furcifera carrying the SRBSDV, comprises the following steps of: feeding the sogatella furcifera by utilizing rice infecting the SRBSDV or cryopreserved rice diseased plant so that the sogatella furcifera obtains the SRBSDV, transferring the infected sogatella furcifera to a healthy rice to be fed till passing through a circulation period of virus, and detecting the sogatella furcifera by utilizing an RT-PCR (reverse transcription-polymerase chain reaction) technology and a serology method to obtain the sogatella furcifera carrying the SRBSDV, wherein the carrier rate of the sogatella furcifera can reach more than 50% through a manual feeding manner based on the experiment. Through utilizing the method for obtaining the sogatella furcifera carrying the SRBSDV, the obtained sogatella furcifera carrying the SRBSDV can be applied to screening of SRBSDV-resisting monoclonal antibody to carry out a rice inoculation experiment to identify the resistance of the rice, screen disease-resistant variety and provide a service of the breeding for disease resistance. The invention can be further applied to researching mutual relation between the SRBSDV and a vector insect sogatella furcifera and provides powerful theoretical evidence for prevention and treatment on the viruses.

Description

technical field [0001] The field of the invention belongs to the field of biotechnology, and in particular relates to a method for obtaining white-backed planthoppers carrying southern rice black-streaked dwarf virus and its application. Background technique [0002] Southern rice black-streaked dwarf virus (SRBSDV) is a virus transmitted by the white-backed planthopper. Once the disease occurs in rice, the loss will be serious. Since SRBSDV was first discovered in Yangxi County, Guangdong Province in 2001, its damage has become increasingly serious. Especially after the 1990s, the large increase in the planting area of ​​hybrid rice and the large-scale and high-intensity application of imidacloprid made the white-backed planthopper gradually replace the brown planthopper and become the dominant population, resulting in successive outbreaks of white-backed planthoppers in most rice areas in southern my country in recent years. In 2008, the southern rice black-streaked dw...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G16/00A01G7/00A01G22/22
Inventor 周雪平吴建祥刘欢徐毅周文武饶黎霞倪跃群祝增荣
Owner ZHEJIANG UNIV
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