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Method for laodelphax striatellus to obtain rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves

A technology of rice black bar dwarfing and freezing method, which is applied in the field of agricultural science, can solve the problems of poor growth, limited breeding of resistant varieties, and difficulty in long-term storage, so as to overcome technical bottlenecks and extend the time for identification of variety resistance Effect

Inactive Publication Date: 2011-04-13
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

However, diseased rice or wheat plants are often severely dwarfed, poor in growth, and difficult to preserve for a long time.
This makes the resistant variety breeding of rice black-streaked dwarf disease very limited, and it is urgent to invent a method for preserving the virus source of rice black-streaked dwarf virus, which can not only preserve the virus source for a long time, but also conveniently Obtain virus from SBPH for inoculation, to assist in the establishment of a more convenient and easy identification method for rice black-streaked dwarf resistance, and to explore and screen resources for resistance to black-streaked dwarf, breed selection and genetic rules Research provides basic research tools

Method used

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  • Method for laodelphax striatellus to obtain rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves
  • Method for laodelphax striatellus to obtain rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves
  • Method for laodelphax striatellus to obtain rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves

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Embodiment 1

[0017] Embodiment 1, the method that SBPH obtains rice black-streaked dwarf virus from frozen wheat diseased leaves

[0018] ① Obtaining leaves of plants carrying rice black-streaked dwarf virus.

[0019] In May 2009, wheat with typical "green dwarf" symptoms was collected in the severe wheat disease area of ​​Pizhou, Jiangsu. Total wheat RNA was extracted according to the instructions of Trizol (Reagent). Primers were designed according to the published S9 sequence of RBSDV (primers were synthesized by Shanghai Handsome Biotechnology Co.):

[0020] R: 5′-GGATTACAACAHACACAMCGAAA-3′

[0021] F1: 5'-GRTAGACAGGCAAAYMTAAGCGT-3'.

[0022] RT-PCR was used to determine whether the diseased plants carried rice black-streaked dwarf virus. Reverse transcription was performed according to the instruction of M-MLV reverse transcriptase (Promega). The PCR amplification system is: 3.0 μL of cDNA template, 0.5 μL of Taq plus enzyme, 0.5 μL of dNTP (10 mmol. -1) 1.0 μL, 1.0 μL of 3’ end...

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Abstract

The invention provides a method for laodelphax striatellus to obtain a rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves. The principle of the invention is as follows: storing plant leaves carrying RBSDV by using a refrigerated method, obtaining RBSDV for laodelphax striatellus from refrigerated diseased leaves by a feeding method, and obtaining laodelphax striatellus withRBSDV applied in inoculating and identification after laodelphax striatellus passes a circulative period of virus. The varietalresistance identification method established by the invention can overcome the technical bottleneck of difficult long-term storage of diseased plants by adopting the method of obtaining the virus which feeds laodelphax striatellus with diseased plant better, broadens the varietalresistance identification time, and is hopeful for quickening the screening speed of disease-resistant resources and breeding process of disease-resistant varieties.

Description

technical field [0001] The invention relates to a method for obtaining rice black-streaked dwarf virus from frozen diseased leaves by brown planthopper and its application in agriculture, belonging to the field of agricultural science and technology. Background technique [0002] Rice black-streaked dwarf virus (Rice black-streaked dwarf virus, RBSDV) is a viral disease transmitted by the striatellus striatellus. It infects rice and causes rice black-streaked dwarf disease. Once the rice is infected, it will not be harvested. Since the disease was discovered in Yuyao County, Zhejiang Province, my country in 1963, it has mainly occurred in indica rice-producing areas in southern Zhejiang in the last century, but it has rarely been found in major japonica rice-producing areas such as Jiangsu. In early 2004, rice stripe blight broke out in Jiangsu and other provinces, and a batch of disease-resistant varieties were rapidly popularized and applied on a large scale, which played ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12R1/94
Inventor 周彤吴丽娟周益军王英程兆榜熊如意季英华
Owner JIANGSU ACAD OF AGRI SCI
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