Molecular marker of rice brown planthopper major gene qBph30(t) and application thereof

A major gene and molecular marker technology, applied in the field of plant molecular genetics, can solve the problems of increasing rice production cost, loss of brown planthopper resistance, brown planthopper re-emergence, etc., and achieves the effects of shortening the breeding cycle, convenient identification and clear location.

Active Publication Date: 2015-02-04
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the use of chemical insecticides to control the hazards of brown planthoppers is the most important control method in agricultural production. This method not only increases the cost of rice production, but also kills the natural enemies of brown planthoppers, which is likely to cause the outbreak of brown planthoppers again. Ecological factors such as pollution, as well as the accumulation of toxicity in the biological chain and corresponding human health problems; and the use of resistance genes of host plants to breed and promote the planting of brown planthopper-resistant rice varieties is the most economical

Method used

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  • Molecular marker of rice brown planthopper major gene qBph30(t) and application thereof
  • Molecular marker of rice brown planthopper major gene qBph30(t) and application thereof
  • Molecular marker of rice brown planthopper major gene qBph30(t) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Acquisition of Molecular Markers

[0026] (1) Anti-Mosquito Blue Zhan / 05BPH16 F 2 Population construction and phenotyping

[0027] (1) The source of resistance 05BPH16 is derived from the resistant offspring of Guangxi common wild rice and cultivated rice hybrids and backcrosses with high resistance to brown planthopper. Populations have high resistance properties. In order to find simple and effective molecular markers that are closely linked to qBph30(t), the present invention crosses the susceptible variety Kangjingqingzhan as the female parent and the brown planthopper resistant strain 05BPH16 as the male parent, and the obtained F 1 and then self-intersection, thereby constructing the F 2 Separate groups; each F 2 A single plant obtains the corresponding F by selfing 2:3 family lineage.

[0028] (2) Adopt seedling stage inoculation to parents, F 2:3 Families were tested for insect resistance. To ensure that the parent and F 2:3 Each family in th...

Embodiment 2

[0044] Example 2: Verification of Molecular Markers

[0045] 1. Materials and methods

[0046] 1.1 Materials

[0047] Negative varieties: insect-susceptible varieties Kangqianqingzhan, 9311, Nipponbare, Baimao, and Bai R54 are all rice materials preserved in our laboratory, and the insect-susceptible materials in the 9311×05BPH16 breeding combination, a total of 30 copies

[0048] Positive varieties: 30 insect-resistant families in the offspring of the hybrid combination of high-resistant rice material 05BPH16 and Kangmoqingzhan×05BPH16.

[0049] The upstream primer of the primer pair for amplifying the molecular marker is the sequence shown in Seq ID NO.1, and the downstream primer of the primer pair is the sequence shown in Seq ID NO.2;

[0050] Seq ID NO.1: 5'-AGCCCAAATGGAACAAACAA-3'

[0051] Seq ID NO.2: 5'-GCTCACGGTTAAGCAATGGT-3'.

[0052] 1.2 Method

[0053] Genomic DNA of rice leaves was extracted by CTAB extraction method, and the extracted sample DNA was amplifie...

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Abstract

The invention discloses a molecular marker of rice brown planthopper major gene qBph30(t) and an application thereof. In the invention, a close-linkage molecular marker of the resistant gene qBph30(t) in an insect-resistant strain 05BPH16 is obtained through a genotype of each single plant of the second filial generation obtained through hybridization between the rice insect-resistant strain 05BPH16 (male) and mosquito-resistant Qingzhan (female) with genetic linkage analysis with combination of anti-brown planthopper resistance levels in each family in F2:3. The molecular marker can effectively detect whether the resistant major gene exists in the insect-resistant strain 05BPH16 and derived varieties (or strains) or not and can quickly improve selective efficiency of an anti-brown planthopper rice material, thereby obtaining an anti-brown planthopper rice variety containing the qBph30(t) gene.

Description

technical field [0001] The invention belongs to the field of plant molecular genetics, and in particular relates to a molecular marker of rice rice main gene qBph30(t) resistant to brown planthopper and its application in breeding rice varieties resistant to brown planthopper. Background technique [0002] Rice brown planthopper (Nilaparvata lugens BPH for short) belongs to the Homoptera: Delphacidae insects, and is one of the most important insect pests in the rice planting process. It has piercing-sucking mouthparts, which directly pierce the phloem of the vascular bundles of the plant to absorb nutrients such as soluble sugars, inorganic salt ions, and amino acids in the juice, hindering the normal growth of rice plants. In severe cases, the whole plant will die and the grains will not be harvested. It is called "planthopper fire"; at the same time, the insect body acts as a medium for pathogens and spreads a variety of rice diseases, such as viral diseases, leaf dwarf ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李容柏邱永福覃宝祥焦晓真杨萌薛艳霞刘芳
Owner GUANGXI UNIV
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