Molecular marker of anti-nilaparvata-lugens major gene Bph3 of rice

A brown planthopper resistance and molecular marker technology, applied in the field of molecular genetics, can solve problems such as difficult aggregation of insect-resistant genes, and achieve the effects of convenient identification, clear main gene location, and convenient and rapid detection.

Inactive Publication Date: 2011-01-26
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] Due to the complexity of insect resistance identification, it is often difficult to

Method used

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  • Molecular marker of anti-nilaparvata-lugens major gene Bph3 of rice
  • Molecular marker of anti-nilaparvata-lugens major gene Bph3 of rice
  • Molecular marker of anti-nilaparvata-lugens major gene Bph3 of rice

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Embodiment Construction

[0033] Studies have shown that BPH-resistant gene resources mainly exist in indica and wild rice species in Sri Lanka and India (Ikeda and Vaughau, 1991). Athwal et al. (1971) reported that Mudgo, CO22 and MTU15 carried the same brown planthopper resistance gene Bph-1, and ASD7 carried a recessive insect resistance gene bph-2. Athwal and Pathak (1972) reported that MGL2 contains the insect resistance gene Bph-1, and Ptb18 contains bph-2. Martinez and Khush (1974) reported that IR747B2-6 contained Bph-1, and R1154-243 and IR4-93 contained bph-2. Lakshiminarayana and Khush (1977) reported that the Sri Lankan insect-resistant variety Rathu Heenati is controlled by a dominant gene Bph-3 that segregates independently from Bph-1; while the variety Babawee is controlled by a recessive gene bph-4 that segregates independently from bph-2 . Sidhu and Khush (1978) reported that rice varieties carrying Bph-3 or bph-4 were resistant to all BPH biotypes. The Thai rice varieties Col.5 Tha...

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Abstract

The invention relates to a molecular marker of an anti-nilaparvata-lugens major gene Bph3 of rice and belongs to the technical field of genetic breeding. A genetic linkage analysis is preformed on a genotype of each single plant of F2 obtained by crossing an rice anti-insect variety RathuHeenati (female) with an insect-susceptible variety 02428 (male) as well as on an anti-nilaparvata-lugens level of each family of F2: 3, thereby obtaining the anti-nilaparvata-lugens major gene Bph3 of the anti-insect variety RathuHeenati. The gene is positioned between the molecular marker A4 and the molecular marker RM16533; the selection efficiencies of three Indel markers RH784, RH786 and RH007 in the interval are around 97%. The molecular marker of the anti-nilaparvata-lugens major gene is used for detecting whether the major gene is contained in the anti-insect variety RathuHeenati as well as derived varieties thereof (family), thereby forecasting the anti-nilaparvata-lugens level; therefore, the selection efficiency of the anti-nilaparvata-lugens rice is improved greatly.

Description

[0001] The present invention is a divisional application. The name of the original application is: Molecular marker method for rice varieties resistant to brown planthopper main gene Bph3. Application date: 2008.12.12 Application number: 200810243722.4. 1. Technical field [0002] The invention provides a molecular marker method for rice varieties resistant to brown planthopper main gene Bph3, belongs to the field of molecular genetics, and is specially used for the selection of rice varieties resistant to brown planthoppers and the utilization of germplasm resources. 2. Background technology [0003] Rice is an important food crop in my country. The brown planthopper is a monophagous pest of rice, belonging to the family Plantagoidae of the order Homoptera. It is a typical piercing-sucking pest that sucks the phloem sap of rice stems through the stylet. In severe cases, the lower part of the rice plant will turn black, rotten and smelly, and paralyzed, which is called "lic...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 万建民刘裕强江玲吴寒何俊刘世家刘喜陈亮明
Owner NANJING AGRICULTURAL UNIVERSITY
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