Molecular mark method for rice variety anti-brownspot gene site

A technology for resisting brown planthoppers and genetic loci, applied in the field of molecular genetics, can solve problems such as difficult aggregation of insect-resistant genes, and achieve the effects of convenient identification, clear location, and cost saving

Inactive Publication Date: 2007-01-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] Due to the complexity of insect resistance identification, it is often difficult to

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  • Molecular mark method for rice variety anti-brownspot gene site

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[0043] The detailed description of the present invention is as follows:

[0044] Studies have shown that BPH-resistant gene resources mainly exist in indica and wild rice species in Sri Lanka and India (Ikeda and Vaughau, 1991). Athwal et al. (1971) reported that Mudgo, CO22 and MTU15 carried the same brown planthopper resistance gene Bph-1, and ASD7 carried a recessive insect resistance gene bph-2. Athwal and Pathak (1972) reported that MGL2 contains the insect resistance gene Bph-1, and Ptb18 contains bph-2. Martinez and Khush (1974) reported that IR747B2-6 contained Bph-1, and R1154-243 and IR4-93 contained bph-2. Lakshiminarayana and Khush (1977) reported that the Sri Lankan insect-resistant variety Rathu Heenati is controlled by a dominant gene Bph-3 that segregates independently from Bph-1; while the variety Babawee is controlled by a recessive gene bph-4 that segregates independently from bph-2 . Sidhu and Khush (1978) reported that rice varieties carrying Bph-3 or b...

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Abstract

The present invention belongs to the molecular genetics field and relates to the molecular marker method of the major gene for brown planthopper resistance in the rice variety Rathu Heenati. Via genetic linkage analysis of the individual F2 plant genotype and the brown planthopper resistance level of each F2:3 family produced by hybridizing the insect-resistant variety Rathu Heenati with insect-susceptible variety 02428, the molecular markers RM8213 and RM5953 of the major gene loci Qbph4(Bph3) in the insect-resistant variety Rathu Heenati are obtained, the loci Qbph4(Bph3) is 3.6cM and 3.2cM distant from the markers RM8213 and RM5953 respectively. Detection of this major gene loci in Rathu Heenati and its derivatives via these molecular markers will increase the selection efficiency of brown plant hopper resistant rices.

Description

1. Technical field [0001] The invention provides a molecular marker method for the rice variety Rathu Heenati resistant to brown planthopper gene loci, belongs to the field of molecular genetics, and is specially used for the breeding of rice varieties resistant to brown planthopper and the utilization of germplasm resources. 2. Technical Background [0002] Rice is an important food crop in my country. The brown planthopper is a monophagous pest of rice, belonging to the family Plantagoidae of the order Homoptera. It is a typical piercing-sucking pest that sucks the phloem sap of rice stems through the stylet. In severe cases, the lower part of the rice plant will turn black, rotten and smelly, and paralyzed, which is called "lice burning", resulting in reduced or lost rice production. At present, the control of brown planthopper mainly depends on chemical pesticides, but this measure not only increases the production cost, but also pollutes the environment. Therefore, th...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 万建民江玲孙立宏刘裕强陈亮明刘世家刘喜王春明程遐年翟虎渠
Owner NANJING AGRICULTURAL UNIVERSITY
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