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Molecular label closely linked with wheat gibberellin resistance main effect QTL and its application

A wheat scab and molecular marker technology, applied in the fields of wheat breeding and molecular biology, to improve breeding efficiency and reduce waste

Inactive Publication Date: 2003-09-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

And Jiangsu Liyang Wangshuibai (Wheat Scab Research, edited by Lu Weizhong, etc., Science Press, Beijing, 2001) is a unique local variety in my country. It has high and stable scab resistance, and it has also been discovered so far. The wheat variety with the strongest resistance to wheat head blight has no molecular markers linked to its main QTL.

Method used

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  • Molecular label closely linked with wheat gibberellin resistance main effect QTL and its application
  • Molecular label closely linked with wheat gibberellin resistance main effect QTL and its application

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Experimental program
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Embodiment 1

[0020] Acquisition of molecular markers closely linked to main QTL for wheat head blight resistance: (1) Construction of Wangshuibai recombinant inbred line population and identification of red blight resistance:

[0021] F 1 bastard, F 1 F 2 Offspring, F 2 The 104 individual plants of 1 randomly select a seed to plant, and adopt the method of single-seed transmission to propagate to F 7 generation, resulting in 104 families.

[0022] The scab resistance inoculation identification was carried out in the greenhouse or in the field respectively, and the spikelets that had just bloomed in the middle of the wheat ears were selected and inoculated with 10 microliters (50,000 spores / ml) of the conidia suspension of Fusarium graminearum strain F34, mist Moisturize for 3 days, and investigate the rate of diseased spikelets after 21 days.

[0023] Diseased spikelet rate=(number of diseased spikelets / total number of spikelets)×100%. (2) Molecular marker analysis, genetic map const...

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Abstract

The molecular label closely linked with the active QTL of wheat fusarium blight resistance is prepared through hybridizing the disease-resistant wheat with the disease-sensitive wheat to obtain recombinant selfing line, and performing QTL and genetic linkage analysis to obtain the said molecular labels Xgwin 161, BARC 147 and Xgwin 493. They can be used to predict the level of the resistance to fusarium blight by detecting whether the DNA of wheat contains the said molecular labels or not.

Description

technical field [0001] The invention relates to the fields of wheat breeding and molecular biology, in particular, selecting and utilizing molecular markers closely linked to main effect QTLs for resistance to scab resistance of wheat for assisted breeding, so as to improve the efficiency of wheat scab resistance breeding. Background technique [0002] Wheat head blight is an important disease in warm and humid wheat regions, mainly caused by Fusarium graminearum. Wheat head blight not only causes serious yield loss, but also mycotoxins such as deoxynivalenol (DON) remaining in the diseased wheat grains seriously threaten the health of humans and animals. Breeding disease-resistant varieties is the most economical way to control this disease. and effective means. A lot of previous work (Bai Guihua, Shanghai Agricultural Journal, 1989, 5(4): 17-23; Sinijders CHA, Euphytica, 1990, 50: 11-18; SinghRP, Plant Disease, 1995, 79: 238-240; van Ginkel M, Plant Disease, 1996, 80:863...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12P19/34C12Q1/68
Inventor 周淼平陆维忠张旭任丽娟马鸿翔
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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