Rice brown planthopper resistant major gene Bph30 molecular marker and application thereof

A technology of resisting brown planthopper and main gene, applied in the field of molecular genetics, can solve environmental problems such as rice pollution

Active Publication Date: 2017-09-26
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the damage of brown planthoppers mostly occurs during the grain filling stage of rice, if a large amount of insecticides are used for control at this time, it will cause serious pollution to the environment and rice.

Method used

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  • Rice brown planthopper resistant major gene Bph30 molecular marker and application thereof
  • Rice brown planthopper resistant major gene Bph30 molecular marker and application thereof
  • Rice brown planthopper resistant major gene Bph30 molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] [Example 1] Acquisition of a molecular marker closely linked to the main gene Bph30 for resistance to brown planthopper in rice

[0061] (1) Segregation population construction and phenotypic identification

[0062] (1) In 1979, the International Rice Research Institute (RRI) found that the rice variety AC1613 was resistant to three brown planthopper populations (biotypes 1, 2, and 3) through insect resistance screening. In 2013, it was identified by the seedling stage group method. The resistance of the variety to brown planthopper was found to be highly resistant to the brown planthopper population in Wuhan, and the resistance level was level 1. In order to locate the resistance gene and find simple and effective molecular markers closely linked to the gene, the present invention uses the susceptible variety 9311 as the female parent and the brown planthopper resistant variety AC1613 as the male parent to cross, and the obtained F 1 ;F 1 F 2 Segregation Groups, Par...

Embodiment 2

[0088] [Example 2] Verification of molecular markers

[0089] 1. Materials and methods

[0090] 1.1 Materials

[0091] Resistant parent AC1613, susceptible parent 9311, two heterozygous 9311 / AC1613BC in the positioning interval 2 f 2 Groups (B4-25, B4-28) have 84 and 75 trees respectively.

[0092]Molecular marker primers: RM16290, SSR-28, RM16294, SSR-68, SSR-69, RM16299, Indel-15, Indel-16, SSR-35, RM16303.

[0093] 1.2 Method

[0094] The resistant parent AC1613, the susceptible parent 9311 and two BC 2 f 2 The phenotype of each individual plant of the population (the method is the same as in Example 1).

[0095] Genomic DNA of rice sample was extracted by CTAB extraction method (method is the same as in Example 1). Sample DNA was amplified with primers RM16290, SSR-28, RM16294, SSR-68, SSR-69, RM16299, Indel-15, Indel-16, SSR-35, RM16303, respectively. The reaction system included 0.10 μM primers, 250 μM dNTPs, 1× PCR reaction buffer (50 mM KCl, 10 mM Tris-HCl pH8...

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Abstract

The invention provides a rice brown planthopper resistant major gene Bph30 molecular marker and application thereof. A genotype of each F2 single plant obtained by hybridization of a rice insect-resistant variety AC1613 (male parent ) and a susceptible variety 9311 (female parent ) and a brown planthopper resistant phenotype of each F2 single plant is combined for genetic linkage analysis to obtain an insect-resistant variety AC1613 resistance gene Bph30 positioned between molecular markers RM16290 and RM16303, and molecular markers in linkage with the gene also include SSR-28, RM16294, SSR-68, SSR-69, RM16299, Indel-15, Indel-16 and SSR-35. By the molecular marker, whether a locus of the major gene exists in rice or not can be effectively detected, and accordingly brown planthopper resistant rice selection efficiency is greatly improved, and brown planthopper resistant rice varieties with the gene Bph30 can be obtained.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a molecular marker of the rice rice main gene Bph30 resistant to brown planthopper and the application of the molecular marker in breeding rice varieties resistant to brown planthopper. Background technique [0002] Rice is one of the most important food crops in the world. Rice production is seriously threatened due to the attack of different pathogens and pests. Among them, the brown planthopper is one of the most serious insect pests in the rice planting process. Its adults and nymphs suck the juice in the phloem of the rice with needles, which causes the leaves of the plant to turn yellow. No harvest. According to the China Agricultural Yearbook, in the six years of 1966, 1969, 1973, 1977, 1983 and 2003, the national brown planthopper had a large outbreak; , the occurrence area of ​​brown planthopper has reached more than 50% of the total rice planting area, which has caused...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 何光存石少阶杜波陈荣智祝莉莉
Owner WUHAN UNIV
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