Method for quickly selecting paddy rice with sheath blight resistance and qSBRLEYD2A genes

A rapid selection, sheath blight technology, applied in plant genetic improvement, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of low accuracy of inoculation identification results, large result errors, poor accuracy and so on

Inactive Publication Date: 2018-05-25
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because most of the traditional inoculation identification is carried out under field planting conditions, the results of inoculation identification have large e

Method used

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  • Method for quickly selecting paddy rice with sheath blight resistance and qSBRLEYD2A genes
  • Method for quickly selecting paddy rice with sheath blight resistance and qSBRLEYD2A genes
  • Method for quickly selecting paddy rice with sheath blight resistance and qSBRLEYD2A genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Example 1: Application of D223 marker in F6 generation segregation population of rice variety Lemont and rice variety Yangdao No. 4 hybrid combination.

[0008] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F6 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F6 segregation population including 219 lines was sown in May 2013 at the China Rice Research Institute Experimental Field in Fuyang District, Hangzhou.

[0009] 2. Use the marker D223 to perform PCR detection on each strain of the segregated population of 219 strains, and use the characteristics of the linkage between the marker D223 and the gene qSBRLEYD2A to detect which strain carries the disease res...

Embodiment 2

[0013] Example 2: Application of D223 marker in F9 generation segregation population of rice variety Lemont and rice variety Yangdao No. 4 hybrid combination.

[0014] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F9 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F9 segregation population including 219 lines was sown in May 2015 at the Experimental Farm of China Rice Research Institute in Fuyang District, Hangzhou.

[0015] 2. Use the marker D223 to perform PCR detection on each strain of the segregated population of 219 strains, and use the characteristics of the linkage between the marker D223 and the gene qSBRLEYD2A to detect which strain carries the disease r...

Embodiment 3

[0019] Example 3: Application of D223 marker in F11 generation segregation population of rice variety Lemont and rice variety Yangdao No. 4 hybrid combination.

[0020] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F11 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F11 segregation population including 219 lines was sown in May 2016 at the China Rice Research Institute Experimental Station in Fuyang District, Hangzhou.

[0021] 2. Use the marker D223 to perform PCR detection on each strain of the segregated population of 219 strains, and use the characteristics of the linkage between the marker D223 and the gene qSBRLEYD2A to detect which strain carries the diseas...

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Abstract

The invention discloses a method for quickly selecting paddy rice with sheath blight resistance and qSBRLEYD2A genes. The method includes carrying out PCR (polymerase chain reaction) amplification ondifferent strains in segregation population of recombinant inbred lines by the aid of D223 labels; selecting strains with banding patterns consistent with No.4 Yangdao which is a parent. Lemont and the No.4 Yangdao are assembled to obtain the segregation population of the recombinant inbred lines. The selected strains are strains with qSBRLEYD2A alleles with sheath blight resistance. Compared withthe traditional field inoculation identification methods, the method has the advantages that the method is simple, convenient and speedy, and stable paddy rice materials with sheath blight resistancecan be selected without inoculation identification; the method is high in selection accuracy as compared with field inoculation methods, and the breeding efficiency can be greatly improved.

Description

technical field [0001] The invention relates to a method for quickly selecting sheath blight-resistant rice carrying qSBRLEYD2A gene by using D223 markers. The method can quickly select new strains resistant to rice sheath blight by using D223 markers. Background technique [0002] Rice production is an important food crop related to my country's national food security. The traditional methods of breeding sheath blight-resistant rice in my country's rice breeding community are all through phenotypic selection, that is, inoculating and identifying rice plants with the pathogenic bacteria of sheath blight at different rice developmental stages. Because most of the traditional inoculation identification is carried out under field planting conditions, the results of inoculation identification have large errors and poor accuracy, and the inoculation results of different years are different, resulting in low accuracy of inoculation identification results. The selection of rice sh...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895A01H1/02A01H1/04
CPCC12Q1/6895A01H1/02A01H1/04C12Q2600/13
Inventor 季芝娟杨长登曾宇翔梁燕
Owner CHINA NAT RICE RES INST
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