Molecular marker of rice long and slender grain gene, and application thereof

A molecular marker, rice technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of inability to carry out early selection, stability, deviation in phenotype observation, etc., to improve breeding efficiency, The effect of saving time and cost and speeding up the breeding process

Pending Publication Date: 2020-01-14
RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are the following disadvantages: early selection cannot be carried out; the observation of the phenotype by the naked eye is biased; it is impossible

Method used

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  • Molecular marker of rice long and slender grain gene, and application thereof
  • Molecular marker of rice long and slender grain gene, and application thereof
  • Molecular marker of rice long and slender grain gene, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present invention provides a technical scheme: a molecular marker for rice slender-grain gene, named SG7-18, including the following steps of obtaining:

[0027] (1) Crossing the long-grain variety Taifeng B as the female parent and the short-grain variety Tesan'ai 2 as the male parent to obtain F 1 , F 1 F 2 seed. Plant F 2 Population, and use the single seed transmission method to obtain a recombined inbred population composed of 170 families;

[0028] (2) Using CTAB method to extract genomic DNA from two parents and 170 families of recombinant inbred lines. Screen the rice SSR markers with polymorphism between the two parents, use these polymorphic SSR markers to perform genotype analysis on the recombinant inbred line population, obtain the population genotype data, and use MapMaker 3.0 to construct a molecular genetic map;

[0029] (3) The grain length, grain width and aspect ratio phenotypes of the isolated population were investigated and counted. WinQTL Cartograph...

Embodiment 2

[0037] The present invention provides a technical scheme: an application of molecular markers for rice slender-grain type genes, specifically the application of SG7-18 molecular markers in detecting the SG7 genotype of rice varieties, the steps are as follows:

[0038] (1) Select representative rice varieties widely used in production and research, including three-line restorer lines (Huazhan, Guanghui 122, Guanghui 1002), three-line maintainer lines (Taifeng B, Bo B, Wufeng B, Yuefeng B, Anfeng B, Guang 8B, Qiu B), conventional rice varieties (Tesanai 2, Yuzhenxiang, Huanghuazhan) and rice model varieties Nipponbare and 93-11. Among them, Taifeng B is a long-grain control variety, and Tesan'ai is a short-grain control variety;

[0039] (2) Two weeks after planting seedlings, half of the leaves of the seedlings of the above rice varieties were taken. Use simple CTAB method to extract rice genomic DNA and dissolve it in 200μL ddH 2 O;

[0040] (3) Using primer pairs SG7-18F and SG7-...

Embodiment 3

[0046] The present invention provides a technical scheme: an application of molecular markers for rice slender-grain genes, specifically the application of SG7-18 molecular markers in cultivating slender-grain rice varieties, the steps are as follows:

[0047] (1) Select the short-grain variety Zao S as the female parent, and the long-grain variety Taifeng B as the male parent. 1 Generation seed

[0048] (2) Plant F 1 Plant, with F 1 The plant is the male parent, and the early S is the female parent. Backcross to get BC 1 F 1 ;

[0049] (3) Planting BC 1 F 1 Plants, 2 weeks after transplanting, pick out half of rice seedling leaves, extract genomic DNA using simple CTAB method, and dissolve it in 200μL ddH 2 O;

[0050] (4) Use primer pair SG7-18F and SG7-18R to BC 1 F 1 Plant DNA is amplified by PCR. The amplification conditions are as follows: PCR amplification reaction system is 20μL, of which: 10×buffer (containing Mg 2+ ) 2μL, 2.5mM dNTPs 2μL (10mmolL -1 ), 2μM forward primer 1μL...

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Abstract

The invention discloses a molecular marker of rice long and slender grain gene as well as application thereof. The molecular marker of the rice long and slender grain gene is prepared by the followingsteps: carrying out hybridization by taking a long and slender grain variety, namely Tai-feng B, as a female parent and a short grain variety, namely Te-san-ai No. 2, as a male parent so as to obtainF1; allowing bagged self-pollination of the F1 so as to obtain F2 seeds; planting F2 population, and adopting a single seed descend method so as to obtain recombinant inbred populations composed of 170 families; performing investigation and statistics on grain lengths, grain widths and length-width ratio phenotypes of segregated populations so as to select characteristic plants containing SG7 from the recombinant inbred populations; and then, developing a molecular marker SG7-18 on basis of SG7 candidate genes according to sequence differences of 18 bp insertion/deletion of LOC_Os07g41200 gene between the long and slender grain variety Tai-feng B and the short grain variety Te-san-ai No. 2, thereby obtaining the molecular marker SG7-18 of the rice long and slender grain gene. The molecular marker of the rice long and slender grain gene is used for detecting SG7 genotype in rice varieties; and the rice long and slender grain gene is further used for marking and cultivating long-and-slender-grain-type rice varieties.

Description

Technical field [0001] The invention relates to the technical field of crop molecular marker assisted selection, in particular to a molecular marker of a rice slender grain type gene and its application. Background technique [0002] More than 50% of the global population feed on rice as the staple food. In many countries and regions, people have a preference for slender rice varieties. Rice grain type not only affects the thousand-grain weight and indirectly the yield, but also is an important factor affecting the appearance quality of rice. According to the "National Standard of the People's Republic of China: High-quality Rice GB / T17891-1999", the aspect ratio of high-quality rice shall not be less than 2.8. In production practice, slender-grained rice tends to have advantages in terms of chalkiness, which is characterized by its crystal clear grains and less whiteness, which is widely loved by consumers. Therefore, cultivating long and slender rice varieties is of great si...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘迪林柳武革王丰李金华付崇允廖亦龙朱满山马晓智曾学勤
Owner RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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