Cucumber powdery mildew resistance main effect QTL compact linkage molecule labeling method and applying method

A technology of cucumber powdery mildew and molecular markers, which is applied in the directions of biochemical equipment and methods, and microbial determination/inspection, can solve the problem of low disease-resistance coincidence rate in the field, improve breeding and selection efficiency, improve breeding efficiency, and speed up Select the effect of progress
CN101240342AInactive Publication Date: 2008-08-13SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2008-08-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a molecular marker method closely linked with major QTL for powdery mildew of cucumber resistance, belonging to the field of biological technology. The invention comprises the following steps: hybridizing susceptible variety S94 of powdery mildew of cucumber with susceptible variety S06 of powdery mildew of cucumber, getting hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; separating DNA of individual cucumber leaves and performing PCR amplification; analyzing data and constructing cucumber genetic linkage map for molecular marker; applying to statistical analysis and QTL location; and determining molecular markers F, CSEPGN11, e23m18f and ME11EM9c linked with major QTL for resistance source S06 powdery mildew. The coincidence rate between the identification result of the invention and the field resistance has improved from 94 percent to 100 percent. By the molecular markers linked with major QTL for resistance source S06 powdery mildew, progeny and derived varieties thereof for the specific resistant source can be selected in early generation seedling without the influence of environment condition, saving cost and improving breeding and selection efficiency.
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Description

technical field

[0001] The present invention relates to a molecular marker method and application method in the field of biotechnology, in particular to a molecular marker method and application method of the main effect QTL for resistance to cucumber powdery mildew closely linked. Background technique

[0002] Cucumber powdery mildew (PM, powdery mildew) is caused by obligate living parasitic powdery mildew, and is a common leaf disease in greenhouses and fields; pathogenic powdery mildew mainly includes Podosphaeraxanthii and Golovinomyces cichoracearum) two; in China, the harm of P. xanthii is more common. Powdery mildew has a wide range of pathogenic hosts, and mature spores can spread with the air flow; it is easy to occur in dry and cool seasons, powdery mildew can infect cucumber plant leaves, stems and flowers, and powdery mildew infection during the flowering period can reduce yield by 20-40%. Spraying fungicides and planting resistant varieties are the main defens...

Claims

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