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Cucumber powdery mildew resistance main effect QTL compact linkage molecule labeling method and applying method

A technology of cucumber powdery mildew and molecular markers, which is applied in the directions of biochemical equipment and methods, and microbial determination/inspection, can solve the problem of low disease-resistance coincidence rate in the field, improve breeding and selection efficiency, improve breeding efficiency, and speed up Select the effect of progress

Inactive Publication Date: 2008-08-13
SHANGHAI JIAO TONG UNIV
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  • Abstract
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Problems solved by technology

However, this patented technology represents the action form of the powdery mildew resistance gene controlled by a single gene, and the coincidence rate between the identification result and the field disease resistance is not high

Method used

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  • Cucumber powdery mildew resistance main effect QTL compact linkage molecule labeling method and applying method
  • Cucumber powdery mildew resistance main effect QTL compact linkage molecule labeling method and applying method
  • Cucumber powdery mildew resistance main effect QTL compact linkage molecule labeling method and applying method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Molecular marker method of closely linked major QTL for powdery mildew resistance in cucumber

[0027] (1) Using S06 to cross with cucumber powdery mildew susceptible variety S94 to produce F 1 mongrel.

[0028] (2)F 1 F 2 , F 2 The 252 single plants of the 252 plants were randomly selected for planting a seed, and propagated to F 7 generation, resulting in 224 families.

[0029](3) Select SRAP, SSR, SCAR and STS primer combinations with amplified polymorphisms between the two parents, and use existing methods for 224 families (Wang Gang, Chinese Science C Life Science 2004, 34 (6): 510 ~516; Sakata Y, Theor Appl Genet (Applied Genetic Theory), 2006, 112: 243-250) for analysis, isolate the DNA of cucumber leaves of each recombinant inbred line, and use related sequence amplified polymorphism (SRAP), Microsatellite (SSR), sequence-specific amplification region (SCAR) and sequence tag site (STS) molecular marker primers carry out PCR amplification, and the amplified...

Embodiment 2

[0041] Validation of molecular markers linked to the main QTL for powdery mildew resistance of S06 in a high-generation genetic population:

[0042] Molecular markers closely linked to the major QTL for powdery mildew resistance in cucumber were obtained. The powdery mildew resistance was predicted for some plants of the F8 offspring crossed between S06 and S94. DNA was isolated from their leaves first, and then the marker F , CSEPGN11, e23m18f, and ME11EM9c primers were used to perform polymerase chain reaction (PCR) on these DNAs, and determine whether there were corresponding markers by judging the maps. If there were more than 3 molecular markers, it indicated that the variety or strain belonged to powdery mildew resistant Sexual material, its absence is susceptible material. The powdery mildew resistance of each cucumber plant was then predicted based on these criteria. Then, the actual powdery mildew resistance of the tested samples was measured by inoculating the powde...

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Abstract

The present invention relates to a molecular marker method closely linked with major QTL for powdery mildew of cucumber resistance, belonging to the field of biological technology. The invention comprises the following steps: hybridizing susceptible variety S94 of powdery mildew of cucumber with susceptible variety S06 of powdery mildew of cucumber, getting hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; separating DNA of individual cucumber leaves and performing PCR amplification; analyzing data and constructing cucumber genetic linkage map for molecular marker; applying to statistical analysis and QTL location; and determining molecular markers F, CSEPGN11, e23m18f and ME11EM9c linked with major QTL for resistance source S06 powdery mildew. The coincidence rate between the identification result of the invention and the field resistance has improved from 94 percent to 100 percent. By the molecular markers linked with major QTL for resistance source S06 powdery mildew, progeny and derived varieties thereof for the specific resistant source can be selected in early generation seedling without the influence of environment condition, saving cost and improving breeding and selection efficiency.

Description

technical field [0001] The present invention relates to a molecular marker method and application method in the field of biotechnology, in particular to a molecular marker method and application method of the main effect QTL for resistance to cucumber powdery mildew closely linked. Background technique [0002] Cucumber powdery mildew (PM, powdery mildew) is caused by obligate living parasitic powdery mildew, and is a common leaf disease in greenhouses and fields; pathogenic powdery mildew mainly includes Podosphaeraxanthii and Golovinomyces cichoracearum) two; in China, the harm of P. xanthii is more common. Powdery mildew has a wide range of pathogenic hosts, and mature spores can spread with the air flow; it is easy to occur in dry and cool seasons, powdery mildew can infect cucumber plant leaves, stems and flowers, and powdery mildew infection during the flowering period can reduce yield by 20-40%. Spraying fungicides and planting resistant varieties are the main defens...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 刘龙洲蔡润潘俊松何欢乐姚丹青
Owner SHANGHAI JIAO TONG UNIV
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