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Molecular marker separated from low-polyphenol-oxidase-activity gene QPpo-5D of wheat

A technology of phenol oxidase activity and polyphenol oxidase, which is applied in the field of agricultural biology, can solve the problem that there is no low PPO active site, and achieve the effects of improving breeding efficiency, speeding up the breeding process, and simplifying the selection method.

Active Publication Date: 2020-12-08
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of a low PPO active site on the long arm of wheat 5D chromosome, and the development of a new molecular marker closely linked to this site will lay the foundation for the isolation and effective utilization of this gene

Method used

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  • Molecular marker separated from low-polyphenol-oxidase-activity gene QPpo-5D of wheat
  • Molecular marker separated from low-polyphenol-oxidase-activity gene QPpo-5D of wheat
  • Molecular marker separated from low-polyphenol-oxidase-activity gene QPpo-5D of wheat

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1. Acquisition of Molecular Markers Linked to IDO580-21 Wheat Low PPO Activity Gene

[0035] 1. Plant material

[0036] Yangmai 158 (Suzhongshenzi No. 179) was used as a control with high PPO activity. IDO580-21 ('Cadoux'(PI591905) / / 'Maya74' / M2) was preserved and provided by the Germplasm Resource Evaluation and Innovation Laboratory of the Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, and its grain PPO activity is extremely low . The hybrid combination of IDO580-21 and Yangmai 158 was prepared to construct a recombinant inbred line population.

[0037] 2. Grain PPO activity evaluation method

[0038] In this study, dopa (L-DOPA / MOPS) was used as the substrate, and PPO catalyzed L-DOPA to form quinones, which in turn caused browning. For each material, we selected plump, undamaged, and disease-free grains, weighed them, and put them into 96-well microtiter plates, with 1 grain per well. Eight replicates and a b...

Embodiment 2

[0054] Example 2, the application of molecular marker AX-110939270 in varieties (lines) in the middle and lower reaches of the Yangtze River

[0055] In order to verify the allelic distribution of the AX-110939270 marker in the varieties (lines) in the middle and lower reaches of the Yangtze River, and whether it can be applied to the breeding improvement of the varieties (lines) in the middle and lower reaches of the Yangtze River, this study used the AX-110939270 marker to analyze 53 accessions from the middle and lower reaches of the Yangtze River. Varieties (lines) were screened, and fluorescence scanning results were obtained by reading the fluorescence signals of KASP-labeled amplified products, and the scanning data were analyzed by Kluster Caller software, showing that these varieties contained heterozygous and homozygous high and low PPO activity genotypes. The variety in the X-axis area of ​​the scatter plot is the "FAM" genotype, the variety in the Y-axis area is the...

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Abstract

The invention discloses a molecular marker separated from a low-polyphenol-oxidase-activity gene QPpo-5D of wheat and belongs to the technical field of agrobiology. A recombinant inbred line RIL colony is constructed through hybridizing wheat germ plasm with extremely low polyphenol oxidase activity and wheat germ plasm Yangmai with high PPO activity, and a resistant gene is located to a stained 5DL5-0.76-1.00 section through a wheat molecular marker technology. In order to explore novel molecular markers of the low-PPO-activity gene QPpo-5D, a KASP marker AX-110939270 is developed by using anSNP marker conversion method. The marker, as a novel molecular marker of the low-PPO-activity gene QPpo-5D, can be better applied to assisted selecting of low-PPO-activity varieties of the wheat, sothat shortages of conventional breeding are overcome, a selecting method is simplified, the efficiency of breeding is increased, and thus, a breeding progress of high-whiteness and high-quality varieties is accelerated.

Description

technical field [0001] The invention relates to a molecular marker closely linked with wheat low polyphenol oxidase activity gene QPpo-5D, which can be applied to molecular marker assisted breeding and belongs to the field of agricultural biotechnology. Background technique [0002] my country is one of the largest wheat producing and consuming countries in the world, and flour-based food is an important food for Chinese residents. In the process of flour storage and pasta food processing, the phenomenon of color browning is very common. Browning not only reduces the whiteness of pasta, but also affects its nutritional value. Grain polyphenol oxidase (polyphenol oxidase, PPO) activity is the main cause of flour browning. Under the action of PPO, phenolic substrates react with oxygen to produce quinones, which can undergo self-polymerization in plants or react with amino acids and proteins in cells to produce black or brown deposits, resulting in brown dough Change. PPO a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/13C12Q2600/156
Inventor 蔡瑾吴纪中付必胜刘彩云张巧凤郭炜吴小有
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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