35 results about "Polyphenol oxidase activity" patented technology

The invention discloses a cold baking method for upper tobacco leaves with poor post-autumn baking resistance in a high-altitude tobacco area, which comprises the steps of timely harvesting fresh tobacco leaves, reasonably weaving the tobacco leaves and loading the tobacco leaves into a kang, and baking by adopting a six-stage cold baking process, and comprises a yellowing starting stage, a low-temperature slow yellowing stage, a yellow-dry coordination stage, a safe color fixing stage, a main vein fading stage and a rapid stem drying stage. The cold baking method has the advantages that the appearance quality of the upper tobacco leaves after autumn can be improved; 2, the post-autumn upper tobacco leaf grade structure can be improved; 3, the activity of polyphenol oxidase in post-autumn upper tobacco leaf baking is reduced; 4, the browning proportion and the baking loss proportion of the upper tobacco leaves after autumn can be reduced; and 5, the aroma substance content and sensory quality of the flue-cured tobacco leaves can be improved.

The invention relates to the technical field of vegetable fresh-keeping, in particular to freeze-dried fresh-keeping vegetables and a preparation method thereof. The method comprises a raw material pretreatment stage, blanching enzyme deactivation, fluidized quick-freezing and vacuum freeze-drying, color protection treatment needs to be carried out after cutting in the raw material pretreatment stage, and in the color protection treatment process, a composite color protection solution is adopted to carry out soaking color protection on cut vegetables; the composite color protection liquid comprises ascorbic acid, tea polyphenol, citric acid and deionized water; the activity of polyphenol oxidase (PPO) is directly inhibited through ascorbic acid (reducibility) in the composite color-protecting solution, tea polyphenol is competitively combined with an enzyme activity center and doubly blocks browning reaction, so that the surfaces of freeze-dried vegetables are free of obvious brown spots, the color is close to a fresh-cut state, and the composite color-protecting solution is capable of protecting the surfaces of the freeze-dried vegetables through redox relay in cooperation with vacuum freeze-drying. The retention rate of vitamin C is increased, the nutritional value of vegetables is retained, the safety risk of chemical additives is avoided, and the food safety detection standard is met.

The invention provides a sweet potato leaf powder processing technology with a high nutrition retention rate, which comprises the following steps: S1, putting sweet potato leaves into a dynamic fixation roller, and mixing nitrogen and carbon dioxide by adopting a nitrogen and carbon dioxide mixing generator to form nitrogen and carbon dioxide mixed gas, the nitrogen and carbon dioxide mixed gas is heated to 95 DEG C through a heat exchanger and sprayed out, the oxygen concentration in the dynamic fixation roller is maintained to be smaller than or equal to 0.5%, and the chlorophyll degradation rate of the sweet potato leaves is monitored through a near-infrared sensor by inhibiting the activity of polyphenol oxidase of the sweet potato leaves; according to the method, a weak acid environment is formed through CO2 dissolution to inhibit the activity of polyphenol oxidase and reduce the requirement for fixation temperature, active ingredients are reserved through three-area gradient variable-temperature drying and rapid dehydration and freeze-drying sublimation, energy consumption is reduced by 37%, heat stability is improved through polyphenol glycosylation, the bioavailability of beta-carotene is improved to 80% through nano embedding, and therefore nutrient loss is avoided, and the method is suitable for industrial production. The drying efficiency, color and flavor are improved.

The invention provides a microalgae nutrient solution and application thereof, and belongs to the technical field of agriculture. The invention provides a microalgae nutrient solution. The microalgae nutrient solution comprises nitrogen-fixing blue-green algae and photosynthetic nutrient green algae, the content of algae single cells in the microalgae nutrient solution is greater than or equal to 1.0 * 10 < 6 >. The microalgae nutrient solution provided by the invention, as a microalgae fertilizer, can significantly improve the activities of urease, sucrase and catalase in soil and reduce the activity of polyphenol oxidase in the soil; the content of alkali-hydrolyzable nitrogen in soil is increased, and the nitrogen fertilizer absorption amount of beet is improved; the richness of soil microbial communities can be increased, and the number of actinomycetes and proteobacteria is increased; growth and development of the beet can be remarkably promoted, the plant height, fresh weight, leaf number, chlorophyll content and net photosynthetic rate of the beet can be improved, accumulation of dry matter of beet tuberous roots can be promoted, timely transfer of beet growth centers can be regulated, and then the yield and sugar yield of the beet can be increased.

The invention discloses white tea leaves with a blood fat reducing function and a preparation device thereof, and relates to the technical field of tea processing, the white tea leaves comprise at least three withering cabins, the withering cabins are sequentially communicated from top to bottom, a sealing mechanism is arranged in the communicated area of the withering cabins, a first conveying belt is arranged in each withering cabin, and a second conveying belt is arranged in each withering cabin. The conveying device is used for conveying and placing tea; the temperature and humidity adjusting mechanisms correspond to the withering cabins in number, are arranged in the withering cabins and are used for adjusting the temperature and humidity required by the tea leaves; the number of the illumination parts corresponds to that of the withering cabins, the illumination parts are arranged in the withering cabins and are used for adjusting illumination of tea leaves, and collaborative optimization of physical space and biochemical parameters is formed through vertical series connection design and independent temperature and humidity illumination control of the three stages of withering cabins: in the first stage, blue light induces polyphenol oxidase activity to be improved and EGCG retention rate to be increased; in the third stage, red light and far infrared are combined, so that the synthesis amount of GABA is increased, the content of tea polysaccharide is increased, and directional enrichment of functional components of the white tea is realized.

This invention discloses a plant-derived preservative for Akebia quinata, its preparation method, and its application, belonging to the field of fruit and vegetable preservation technology. The preservative uses young Akebia quinata fruit as the main raw material, combined with Sophora flavescens, Phellodendron chinense, and Zanthoxylum bungeanum, and is prepared through primary yeast fermentation and secondary fermentation with Lactobacillus plantarum and Bacillus amyloliquefaciens. The preservative significantly reduces polyphenol oxidase (PPO) activity, effectively inhibiting enzymatic browning in Akebia quinata; after 14 days of storage at room temperature, the rot rate decreases to 64%-66%, with effects comparable to imazalil; it delays fruit cracking, reduces the cracking rate, and decreases the length and width of the ventral suture; it also has a significant inhibitory effect on Escherichia coli and Staphylococcus aureus. The product of this invention is purely plant-derived, with no chemical residues, and possesses four functions: inhibiting browning, preventing rot, preventing fruit cracking, and broad-spectrum antibacterial activity. The process is stable, and the application is simple, making it suitable for post-harvest green preservation of Akebia quinata and showing good industrialization prospects.

The application discloses a kind of continuous production line for preparing yeast black tea by biological fermentation, including quantitative impurity removal feeder conveyor, withering fermentation machine, kneading and twisting block fermentation machine and hot air drying aroma and vacuum freeze drying all-in-one machine.By this production line, the continuous processing of yeast black tea can be realized, and the temperature of each step in tea processing can be accurately controlled, thereby reducing the loss of polyphenol oxidase activity in tea, and completing the final drying of tea by freeze-drying, and retaining a higher activity of polyphenol oxidase.Therefore, the yeast black tea processed by this production line not only tastes good, but also can be used for fermentation with other plants, and the fermentation of other plants is promoted by polyphenol oxidase in tea, thereby used for the production and processing of composite black tea.It can be widely applied in the field of black tea processing technology.

The invention relates to improvements in reduction of tuber browning in potato (Solanum tuberosum) varieties utilising endonuclease-directed gene editing technologies In particular, the invention provides a Solanum tuberosum plant cell comprising a mutation which generates a phenotype of reduced or eliminated polyphenol oxidase activity when compared to a Solanum tuberosum plant cell without the mutation. The invention further provides ribonucleoprotein complexes comprising an endonuclease and a guide RNA that hybridises with a target sequence comprised within a polyphenol oxidase 2 (StPPO2) gene of a Solanum tuberosum variety, and methods of using the same.

The application discloses a NtPPO gene homozygous mutant and application thereof, and the application is: (1) reducing polyphenol oxidase activity of tobacco leaves; (2) reducing browning degree of tobacco in curing; the NtPPO gene mutant comprises mutant types of NtPPO1, NtPPO2, NtPPO3 and NtPPO4; the NtPPO gene mutant comprises mutant types of NtPPO1, NtPPO2, NtPPO3 and NtPPO4; the nucleotide sequence of the NtPPO1 gene mutant type is shown in SEQ ID NO. 1; the nucleotide sequence of the NtPPO2 gene mutant type is shown in SEQ ID NO. 2; the nucleotide sequence of the NtPPO3 gene mutant type is shown in SEQ ID NO. 3; and the nucleotide sequence of the NtPPO4 gene mutant type is shown in SEQ ID NO. 4.

The invention relates to the technical field of plant effective component extraction, in particular to an extraction method of a freeze-dried fresh rose stock solution, which comprises the following steps: selecting fresh fresh rose without plant diseases and insect pests, removing calyx, pedicel and other impurities, carrying out ultrasonic cleaning for 2-3 times by using a deionized water mixed solution containing citric acid, vitamin C and EDTA-2Na, and carrying out freeze-drying to obtain the freeze-dried fresh rose stock solution. After draining, the water content is controlled to be 86-88% through near infrared spectrum monitoring; wherein the content of citric acid in the deionized water mixed solution is 0.1%-0.3%, the content of vitamin C is 0.05%-0.08%, the content of EDTA-2Na is 0.02%-0.04%, the citric acid inhibits the activity of polyphenol oxidase, the vitamin C blocks an oxidation chain reaction, the EDTA-2Na chelates metal ions and avoids catalytic oxidation of the metal ions, and the oxidation rate of the active ingredients is reduced to 5% or below through synergism of the citric acid, the vitamin C and the EDTA-2Na.

The invention relates to the technical field of rhizoma polygonati treatment, in particular to a low-temperature tumbling system and process for directional softening and browning inhibition of rhizoma polygonati fibers. The system comprises a tumbling barrel, a collecting box, a cooling circulation module and an ultraviolet-ultrasonic co-processing unit. In the rotating process of the tumbling barrel, rhizoma polygonati cells are crushed to release juice, the juice is filtered by the collecting box, is cooled to 0-10 DEG C by the cooling device, and is circularly sprayed to the surface of rhizoma polygonati, so that dynamic temperature control is realized; an ultraviolet lamp inhibits microbial reproduction, ultrasonic waves destroy the activity of polyphenol oxidase, and a browning reaction is blocked in combination with a low-temperature environment; according to the technology, through alternate operation of rolling and standing, the fiber softening rate and the enzyme activity inhibition efficiency are cooperatively regulated and controlled, directional softening of the rhizoma polygonati fibers is achieved under the low-temperature condition, and meanwhile the browning risk is remarkably reduced. The system gives consideration to the quality and processing efficiency of rhizoma polygonati, and is suitable for industrial processing of different varieties such as rhizoma polygonati with rolled leaves and polygonatum kingianum. The method can be widely applied to the technical field of rhizoma polygonati treatment.

The invention discloses an NtPPO gene heterozygous mutant and application thereof. The application comprises the following steps: (1) reducing polyphenol oxidase activity of tobacco leaves; (2) the browning degree of the flue-cured tobacco during baking is reduced; the NtPPO gene mutant comprises mutant types of NtPPO1, NtPPO2, NtPPO3, NtPPO4, NtPPO9, NtPPO10, NtPPO11, NtPPO12 and NtPPO13, and the mutant types of the NtPPO4 are limited as follows: a mutant allele NtPPO4-1 positioned on a first chromosome and a second chromosome and a mutant allele NtPPO4-2 positioned on a third chromosome and a fourth chromosome; the mutant type of the NtPPO9 is limited as follows: a mutant allele NtPPO9-1 positioned on a first chromosome and a second chromosome, and a mutant allele NtPPO9-2 positioned on a third chromosome and a fourth chromosome; the mutant type of the NtPPO10 is limited as follows: a mutant allele NtPPO10-1 positioned on a first chromosome and a second chromosome, and a mutant allele NtPPO10-2 positioned on a third chromosome and a fourth chromosome.

The present application relates to the processing technology field of Rhizoma Polygonati, in particular to a low-temperature tumbling system and process for directional softening and browning inhibition of Rhizoma Polygonati fibers; the system comprises a tumbling barrel, a collection box, a cooling circulation module and an ultraviolet-ultrasonic synergistic treatment unit. The tumbling barrel breaks Rhizoma Polygonati cells and releases juice during rotation, the juice is filtered by the collection box, cooled to 0-10 DEG C by a cooling device, and then recycled and sprayed onto the surface of Rhizoma Polygonati to realize dynamic temperature control; the ultraviolet lamp inhibits the reproduction of microorganisms, the ultrasonic wave destroys the activity of polyphenol oxidase, and the low-temperature environment blocks the browning reaction; the process realizes directional softening of Rhizoma Polygonati fibers under low-temperature conditions by alternating tumbling and standing, synergistically controls the softening rate of fibers and the inhibition efficiency of enzyme activity, and significantly reduces the browning risk. The system takes into account the quality and processing efficiency of Rhizoma Polygonati, is suitable for the industrialized processing of different varieties such as Rhizoma Polygonati and Rhizoma Polygonati, and can be widely applied to the processing technology field of Rhizoma Polygonati.

The present application provides a wheat grain polyphenol oxidase activity major site and SNP marker and application, the SNP molecular marker Marker149631 is located on the 3A chromosome of wheat, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1 or SEQ ID NO.2; the SNP molecular marker Marker149773 is located on the 3A chromosome of wheat, the nucleotide sequence of the molecular marker is as shown in SEQ ID NO.3 or SEQ ID NO.4; the SNP site provided by the present application is of great significance for detecting wheat grain low PPO activity variety, strain, breeding material, and molecular markers can be developed according to it, and the efficiency of wheat quality breeding is accelerated.

The invention discloses a nano antibody combined with polyphenol oxidase and application of the nano antibody. The nucleotide sequence of the nano antibody is as shown in SEQ ID NO.1, and the amino acid sequence corresponding to the nano antibody is as shown in SEQ ID NO.2. The nano antibody prepared by the invention has good stability, high specificity and high affinity, and can accurately inhibit the activity of polyphenol oxidase, thereby fundamentally preventing enzymatic browning; the prepared nano antibody is mainly used as a natural biological preservative and a processing aid, can be widely applied to quality maintenance of aquatic products and various easily browning foods, effectively blocks the enzymatic browning process by specifically inhibiting the activity of polyphenol oxidase, remarkably prolongs the shelf life and maintains the sensory quality of the product.

The present invention discloses the application of a cationic surfactant and mesoporous silica in synergistically inhibiting the activity of polyphenol oxidase. In the present invention, the cationic surfactant DTAB or [C 12 mim]Br is incubated with mesoporous silica nanoparticles together with polyphenol oxidase, which can cause more than 90% loss of the activity of polyphenol oxidase, and the usage amounts of the cationic surfactant DTAB and [C 12 mim]Br are significantly lower than the usage amounts when DTAB or [C 12 mim]Br is used alone.

The invention discloses a processing method of whole-wheat half-dry noodles, and belongs to the field of grain and oil food processing. According to the method, the subacid electrolyzed water with the hypochlorous acid content of 0.2 g / L-0. 5g / L is adopted for dough kneading, and lipase and polyphenol oxidase in the wholewheat noodles are rapidly passivated in combination with catalytic infrared rays, so that the total number of bacterial colonies and the activity of the polyphenol oxidase of the wholewheat half-dry noodles can be remarkably reduced, rancidity and deterioration of the wholewheat half-dry noodles can be inhibited, meanwhile, relatively good eating quality can be ensured, and the quality of the wholewheat half-dry noodles is improved. The shelf life of the prepared whole-wheat half-dry noodles can reach 33 days under the condition of 25 DEG C.

The invention discloses a method for reducing browning degree of flue-cured tobacco leaves, which comprises the following steps: editing target cultivated tobacco by using a gene editing system to obtain tobacco strains with simultaneous mutation of NtPPO1, NtPPO2, NtPPO3, NtPPO4, NtPPO12 and NtPPO13; the activity of polyphenol oxidase in the tobacco leaves is reduced by mutating NtPPOs, so that the browning degree of the cured tobacco leaves is weakened.

The invention discloses a potato variety screening method for processing potato rice noodles by using fresh potatoes, and relates to the technical field of potato variety screening. The specific screening method is as follows: according to screening indexes, varieties suitable for processing the potato rice noodles by using the fresh potatoes are screened from known potato varieties, and the screening indexes are as follows: the ratio of amylose to amylopectin is 0.3-0.4 and the activity of polyphenol oxidase is less than 0.8 U. The screened potatoes are used for preparing the rice flour, and the addition amount of the fresh potatoes is set, so that the defects that the potato dietary fiber content is high, and the ratio of amylose to amylopectin in the potatoes and rice is not matched, so that the adhesiveness of the potatoes and the rice is poor and the rice quality is poor are overcome. The technical bottlenecks of high breaking rate, high cooking loss rate, high viscosity and easy paste of the potato rice noodles during cooking are overcome, and the problem that the comprehensive quality of the potato rice noodles is difficult to improve is solved.

The invention discloses a sterilizing and fresh-keeping method of freshly squeezed fruit juice, which comprises the following steps: adding butanedione not exceeding 0.5 mM into the freshly squeezed fruit juice, irradiating with ultraviolet light for 1-5 minutes, and packaging the fruit juice into a clean and sterile container; according to the method, by inhibiting microbial growth and reducing the activity of polyphenol oxidase, the dual goals of fruit juice sterilization and fresh keeping are achieved, the problems of browning, nutrition loss, flavor change and the like caused by too high processing temperature in fruit juice production are solved, the obtained fruit juice is bright in color and fresh and refreshing in taste, and the original flavor of the fruit juice is reserved to the maximum extent.

The invention provides a preparation method of soybean protein isolate with low beany flavor and high water solution transparency, which comprises the following steps: carrying out hot steam heat treatment on defatted soybean meal to obtain defatted soybean meal subjected to heat treatment; desalted water is added into the degreased soybean meal subjected to heat treatment for protein extraction; after extraction, centrifuging to remove bean dregs, adding sodium pyrosulfite into the extract, adjusting the pH value, centrifuging, and precipitating to obtain protein curd; redissolving the protein curd with water to obtain protein liquid; and mixing the protein liquid with compound protease, performing sterilization, flash evaporation and homogenization treatment, and performing spray drying, thereby obtaining the product. The defatted soybean meal is subjected to superheated steam pretreatment and high-temperature instantaneous treatment to inactivate lipoxygenase and the like, so that beany flavor substances generated by oxidation in the subsequent process are greatly reduced; the added sodium pyrosulfite inhibits the activity of polyphenol oxidase to realize soybean protein bleaching, and the whiteness of the product is improved; the protein solubility and emulsification stability are improved through a compound proteolysis process, and the transparency of the product is improved.

The invention belongs to the technical field of biology, and particularly relates to a black bean exosome and application thereof. The black bean exosome is extracted according to the following steps: soaking black beans in water, performing juicing treatment by taking PBS as a solvent, centrifuging the obtained juice, and collecting supernate; mixing and incubating the supernate and polyethylene glycol, centrifuging, and collecting the precipitate, thereby obtaining the product. Tests prove that the extracted exosome has anti-oxidation and anti-tyrosinase activity, and can be used for preparing functional cosmetics or anti-oxidation drugs.

The invention relates to improvements in reduction of tuber browning in potato (Solanum tuberosum) varieties utilising endonuclease-directed gene editing technologies In particular, the invention provides a Solanum tuberosum plant cell comprising a mutation which generates a phenotype of reduced or eliminated polyphenol oxidase activity when compared to a Solanum tuberosum plant cell without the mutation. The invention further provides ribonucleoprotein complexes comprising an endonuclease and a guide RNA that hybridises with a target sequence comprised within a polyphenol oxidase 2 (StPPO2) gene of a Solanum tuberosum variety, and methods of using the same.

This invention provides the major active site of wheat grain polyphenol oxidase activity and the SNP marker Marker 149773, as well as their applications. The SNP molecular marker Marker 149631 is located on wheat chromosome 3A, and its nucleotide sequence is shown in SEQ ID NO. 1 or SEQ ID NO. 2. The SNP molecular marker Marker 149773 is located on wheat chromosome 3A, and its nucleotide sequence is shown in SEQ ID NO. 3 or SEQ ID NO. 4. The SNP sites provided by this invention are of great significance for detecting wheat varieties, lines, and breeding materials with low PPO activity. Molecular markers can be developed based on these sites to accelerate the efficiency of wheat quality breeding.

The invention belongs to the technical field of wheat molecular breeding, and particularly relates to an SNP site associated with a wheat polyphenol oxidase active major gene. The SNP site has a polymorphic site of A / G allele mutation at the 36th basic group. In the application, the inventor tries to excavate and obtain key genetic loci and genes which can stably exist under multiple environment conditions and control the PPO activity of wheat on the basis of analyzing PPO activity characters of 207 representative wheat materials planted in different years by utilizing a whole genome association analysis method and combining a wheat 660K SNP chip; and the Whaas77577 molecular marker which is closely linked with the major gene qPPO3A.1 related to the PPO activity of the wheat is obtained. Based on the molecular marker, excellent allele variation type identification can be performed on breeding early generation materials, so that a scientific basis can be provided for wheat breeding progeny material selection.

The invention relates to the technical field of fruit preservation, in particular to color protection liquid and a low-temperature freshness locking technology thereof. The invention relates to a composite color protection solution and a low-temperature fresh-keeping technology based on the composite color protection solution, and the specific technical content is as follows: 1, the composite color protection solution comprises two functional mixed solutions which are respectively used for blanching color protection and soaking color protection, and the specific formula is as follows: a first color protection blanching solution is prepared by mixing 0.2-0.5% of sodium alginate and 0.1% of citric acid, and a second color protection blanching solution is prepared by mixing 0.2-0.5% of sodium alginate and 0.1% of citric acid; sodium alginate can form a primary protective film on the surface of litchi, and citric acid can adjust the pH value of the system and inhibit the activity of polyphenol oxidase. The method has the beneficial effects that the preservation cost is low: the composite color protection liquid adopts low-cost raw materials such as sodium alginate and citric acid, the process steps are simple and easy to operate, complex equipment is not needed, and the method is suitable for large-scale industrial production; the fresh-keeping effect is good: through a dual color protection mechanism of blanching color protection and soaking color protection, and in combination with a nitrogen-filling oxygen-isolating and low-temperature quick-freezing technology, browning and rotting of the litchis can be effectively inhibited.

The present invention discloses a continuous tea-fixing machine and an operation method thereof, wherein the continuous tea-fixing machine comprises a feeding impurity removal conveyor belt, an electromagnetic tea-fixing drum, a hot air tea-fixing tank, a wet hot steam recovery heating purification device, and an intelligent control device. The present invention first removes the impurities entrained in the tea leaves through the impurity removal conveyor belt, and then uses the electromagnetic radiation heat energy of the electromagnetic tea-fixing drum to penetrate into the inner layer of the tea leaves for a stuffy tea-fixing, quickly killing the polyphenol oxidase activity in the inner layer of the tea leaves, preventing the red change reaction, and then uses the hot air tea-fixing tank for a secondary penetration tea-fixing, efficiently volatilizing the wet hot gas, promoting the volatilization of aromatic substances and improving the aroma, and can overcome the technical limitation that the existing drum-type tea-fixing machine is difficult to quickly emit and eliminate the wet hot gas that affects the tea-fixing quality. The wet hot gas recovery heating purification device recovers, heats, purifies and reuses the wet hot gas in the hot air tea-fixing tank, thereby effectively improving the utilization rate of heat and achieving energy saving and emission reduction.

To provide hexaploid wheat with low polyphenol oxidase activity and preserving quantitative trait locus Q.Ymym for wheat yellow mosaic disease resistance, and to provide a primer set that enables determination of whether or not a Triticum plant possesses a loss-of-function Ppo-D1 gene.SOLUTION: Provided is a hexaploid wheat having a homozygous type gene which is a Ppo-D1 gene with defective or reduced function of polyphenol oxidase activity and a quantitative trait locus Q.Ymym for resistance to wheat yellow mosaic disease, on chromosome 2D.SELECTED DRAWING: None

The invention discloses an NtPPO gene homozygous mutant and application thereof. The application of the NtPPO gene homozygous mutant comprises the following steps: (1) reducing polyphenol oxidase activity of tobacco leaves; (2) the browning degree of the flue-cured tobacco during baking is reduced; the NtPPO gene mutant comprises mutation types of NtPPO1, NtPPO2, NtPPO3 and NtPPO4, the NtPPO gene mutant comprises mutation types of NtPPO1, NtPPO2, NtPPO3 and NtPPO4, the nucleotide sequence of the NtPPO1 gene mutation type is shown as SEQ ID NO.1, the nucleotide sequence of the NtPPO2 gene mutation type is shown as SEQ ID NO.2, the nucleotide sequence of the NtPPO 3 gene mutation type is shown as SEQ ID NO.3, and the nucleotide sequence of the NtPPO4 gene mutation type is shown as SEQ ID NO.4. The NtPPO gene mutant comprises mutation types of NtPPO1, NtPPO2, NtPPO3 and NtPPO4.

The invention discloses an intelligent tea storage tank and a control method, and belongs to the field of tea fresh-keeping equipment. The storage tank comprises a modularized multi-cavity structure, a multi-mode environment regulation and control system and an intelligent interaction module. The method is characterized by comprising the following steps: multi-tea self-adaptive storage: presetting six special tea storage modes, and realizing accurate environment regulation and control through a temperature and humidity sensor, an electromagnetic adjustable ventilation valve and a semiconductor chilling plate; dynamic intelligent control: dynamically adjusting micro-aerobic circulation based on an AI algorithm and polyphenol oxidase activity detection data, and managing humidity in combination with a centrifugal fan and a molecular sieve adsorption box; according to the modular and bacteriostatic design, the magnetic type detachable cavity is provided with a bacteriostatic inner container; and man-machine interaction: an electronic ink screen displays aging indexes and drinking suggestions, and a mobile phone APP supports remote monitoring and abnormal early warning. The problems that a traditional device is single in function and cannot meet the requirements of various types of tea are solved, the fresh-keeping period of the tea is remarkably prolonged, the quality conversion efficiency of the tea is remarkably improved, and the device is suitable for family and commercial scenes.