Method for keeping cordyceps sinensis fresh by using irradiation method
The technology for fresh Cordyceps sinensis and Cordyceps sinensis is applied in the field of preservation of Cordyceps sinensis, can solve the problems of complicated operation and high equipment dependence, and achieves the effects of reducing the growth rate of polyphenol oxidase activity and the decreasing rate of soluble protein content.
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Embodiment 1
[0063] At 0°C, use linear running water to clean the head, spores and worm bodies of the Cordyceps sinensis with soil collected from the place of production; place the fresh Cordyceps sinensis after cleaning in a 0.1% calcium lactate solution by mass , ultrasonic cleaning 5 times (power 250W, frequency 120Hz, temperature between 0°C, time 5 times), drain the surface moisture at 0°C.
[0064] Preparation of preservation solution: 3mmol / L sorbitol, 3mmol / LVc, 6μmol / L phytic acid, L-cysteine concentration of 0.4mg / kg, 30mg / mL ethanol extract of Cordyceps sinensis fermentation broth, 50mg / L shell oligo Sugar, 100mg / L nisin antimicrobial peptide (Nisin, manufacturer: Shandong Freda Biotechnology Co., Ltd.).
[0065] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes, soybeans, sucrose, glucose, peptone, yeast extract, mineral salts and vitamins) after activ...
Embodiment 2
[0069] At 10°C, use linear running water to clean the head, spores and worm bodies of the Cordyceps sinensis with soil collected from the place of production; place the fresh Cordyceps sinensis after cleaning in a 0.5% calcium lactate solution by mass , Ultrasonic cleaning once (power 1200W, frequency 40kHz, temperature between 10°C, time 5min / time), drain the surface moisture at 10°C.
[0070] Preparation of fresh-keeping solution: 1mmol / L sorbitol, 1mmol / L iso-Vc, 9μmol / L phytic acid, L-cysteine concentration of 0.8mg / kg, 100mg / mL ethanol extract of Cordyceps sinensis fermentation broth, 200mg / L Oligochitosan, 200mg / L Streptomyces antimicrobial peptide (ε-polylysine, ε-Polylysine, manufacturer: Zhengzhou Bainafu Biological Engineering Co., Ltd.);
[0071] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes, soybeans, sucrose, glucose, peptone, yeast e...
Embodiment 3
[0075] At 5°C, use linear running water to clean the head, spores and worm bodies of the Cordyceps sinensis with soil collected from the place of production in sequence; place the fresh Cordyceps sinensis after cleaning in a 0.5% calcium lactate solution by mass , ultrasonic cleaning 3 times (power 600W, frequency 80Hz, temperature between 5°C, time 7min / time), drain the surface moisture at 5°C.
[0076] Preparation of fresh-keeping solution: 2mmol / L sorbitol, 2mmol / L iso-Vc, 8μmol / L phytic acid, L-cysteine concentration of 0.6mg / kg, 60mg / mL ethanol extract of Cordyceps sinensis fermentation broth, 150mg / L Chitooligosaccharides, 150mg / L Pediocin PA-1 (PediocinPA-1, derived from the fermentation broth of Pediococcus P. acidilactici, P. cerevisicae, P. pentosaceus and other Pediococcus species).
[0077] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes...
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