Method for keeping cordyceps sinensis fresh by using irradiation method

The technology for fresh Cordyceps sinensis and Cordyceps sinensis is applied in the field of preservation of Cordyceps sinensis, can solve the problems of complicated operation and high equipment dependence, and achieves the effects of reducing the growth rate of polyphenol oxidase activity and the decreasing rate of soluble protein content.

Active Publication Date: 2019-05-07
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In order to overcome the defects of high dependence on equipment and cumbersome operation in the existing preservation methods of fresh Cordyceps sinensis, the present invention provid

Method used

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  • Method for keeping cordyceps sinensis fresh by using irradiation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] At 0°C, use linear running water to clean the head, spores and worm bodies of the Cordyceps sinensis with soil collected from the place of production; place the fresh Cordyceps sinensis after cleaning in a 0.1% calcium lactate solution by mass , ultrasonic cleaning 5 times (power 250W, frequency 120Hz, temperature between 0°C, time 5 times), drain the surface moisture at 0°C.

[0064] Preparation of preservation solution: 3mmol / L sorbitol, 3mmol / LVc, 6μmol / L phytic acid, L-cysteine ​​concentration of 0.4mg / kg, 30mg / mL ethanol extract of Cordyceps sinensis fermentation broth, 50mg / L shell oligo Sugar, 100mg / L nisin antimicrobial peptide (Nisin, manufacturer: Shandong Freda Biotechnology Co., Ltd.).

[0065] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes, soybeans, sucrose, glucose, peptone, yeast extract, mineral salts and vitamins) after activ...

Embodiment 2

[0069] At 10°C, use linear running water to clean the head, spores and worm bodies of the Cordyceps sinensis with soil collected from the place of production; place the fresh Cordyceps sinensis after cleaning in a 0.5% calcium lactate solution by mass , Ultrasonic cleaning once (power 1200W, frequency 40kHz, temperature between 10°C, time 5min / time), drain the surface moisture at 10°C.

[0070] Preparation of fresh-keeping solution: 1mmol / L sorbitol, 1mmol / L iso-Vc, 9μmol / L phytic acid, L-cysteine ​​concentration of 0.8mg / kg, 100mg / mL ethanol extract of Cordyceps sinensis fermentation broth, 200mg / L Oligochitosan, 200mg / L Streptomyces antimicrobial peptide (ε-polylysine, ε-Polylysine, manufacturer: Zhengzhou Bainafu Biological Engineering Co., Ltd.);

[0071] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes, soybeans, sucrose, glucose, peptone, yeast e...

Embodiment 3

[0075] At 5°C, use linear running water to clean the head, spores and worm bodies of the Cordyceps sinensis with soil collected from the place of production in sequence; place the fresh Cordyceps sinensis after cleaning in a 0.5% calcium lactate solution by mass , ultrasonic cleaning 3 times (power 600W, frequency 80Hz, temperature between 5°C, time 7min / time), drain the surface moisture at 5°C.

[0076] Preparation of fresh-keeping solution: 2mmol / L sorbitol, 2mmol / L iso-Vc, 8μmol / L phytic acid, L-cysteine ​​concentration of 0.6mg / kg, 60mg / mL ethanol extract of Cordyceps sinensis fermentation broth, 150mg / L Chitooligosaccharides, 150mg / L Pediocin PA-1 (PediocinPA-1, derived from the fermentation broth of Pediococcus P. acidilactici, P. cerevisicae, P. pentosaceus and other Pediococcus species).

[0077] Preparation of the ethanol extract of the Cordyceps sinensis fermentation liquid: inoculate the Cordyceps sinensis strain into a liquid fermentation medium (including potatoes...

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Abstract

The invention provides a method for keeping cordyceps sinensis fresh by using an irradiation method, and relates to the technical field of cordyceps sinensis fresh-keeping. The method includes soakingfresh cordyceps sinensis in a fresh-keeping solution; performing modified atmosphere packaging; and performing irradiation on the modified atmosphere packaged cordyceps sinensis with <60>Co[gamma] rays or 5-10 MeV electron beams, irradiation dose being 0.1-1.5 kGy, and ununiformity being less than or equal to 1.25 so that the fresh-keeping of the cordyceps sinensis can be realized. According to the embodiments, compared with the prior art, the weight loss rate, soluble protein content decreasing rate and polyphenol oxidase activity growth rate of the cordyceps sinensis can be significantly reduced by adopting the method to perform fresh-keeping processing on the fresh cordyceps sinensis, and the fresh-keeping time at least can reach 75 d; and long-time and effective fresh-keeping on animal-fungal tissue complexes like the cordyceps sinensis can be realized under limited irradiation dose, and obvious damage to the cordyceps sinensis can be avoided.

Description

technical field [0001] The invention relates to the technical field of preservation of Cordyceps sinensis, in particular to a method for preservation of Cordyceps sinensis by irradiation. Background technique [0002] Cordyceps sinensis is a high-grade nourishing and precious Chinese medicinal material, which contains a variety of nutrients and active ingredients, including nucleosides, polysaccharides, sterols, fatty acids, proteins, peptides and volatile components, etc. It has anti-tumor, immune system regulation, and promotes body cell damage Repair, anti-free radicals, anti-oxidation, anti-aging, protect cardiac muscle and vascular cells, improve cardiovascular function, protect the liver, protect the kidneys, regulate the respiratory system, regulate the blood system and regulate blood lipids and other functions. [0003] Most of the Cordyceps currently on the market are dried Cordyceps. During the drying process of Cordyceps, a large number of volatile components will...

Claims

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Application Information

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IPC IPC(8): A23L3/26A23L3/3562A23L3/3544A23L3/3508A23L3/3535A23L3/3463A23L3/3526A23L3/3418
Inventor 毕宏涛魏立新肖远灿杜玉枝杨红霞
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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