2results about How to "Increase chlorogenic acid content" patented technology

The invention belongs to the technical field of plant cultivation, provides application of bacillus H8 in cultivation of emilia sonchifolia, and particularly relates to bacillus H8, a bacillus H8 suspension with the concentration of 102-104 CFU / mL is prepared and applied to the position 2-3 cm away from the root of emilia sonchifolia as a bio-fertilizer once every 7 days, plant growth can be remarkably promoted, and the yield of emilia sonchifolia is increased. The content of medicinal components of chlorogenic acid and quercitrin in the leaves is increased; the method solves the problems of soil degradation, unstable medicinal material quality and the like caused by dependence on chemical fertilizers and pesticides in traditional cultivation, is suitable for green and efficient cultivation of emilia sonchifolia, and has the advantages of simplicity and convenience in operation, environment friendliness, low cost and the like.

The application belongs to the technical field of tobacco germplasm improvement, and mainly relates to application of a tobacco Nta07g03160 gene in improving chlorogenic acid content in tobacco. The application takes the tobacco Nta07g03160 gene as a target, designs a gRNA sequence of CRISPR / Cas9, connects a DNA fragment containing the above gRNA into a CRISPR / Cas9 vector, then transforms tobacco, obtains edited materials of the tobacco Nta07g03160 gene, and finds that the edited materials can improve the chlorogenic acid content in tobacco.